Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HUZ63_RS19685 Genome accession   NZ_CP054965
Coordinates   4065101..4065541 (+) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli strain STIN_95     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4060101..4070541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUZ63_RS19665 (HUZ63_19455) aroP 4061101..4062471 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  HUZ63_RS19670 (HUZ63_19460) ampE 4062514..4063368 (-) 855 WP_239634630.1 beta-lactamase regulator AmpE -
  HUZ63_RS19675 (HUZ63_19465) ampD 4063365..4063916 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUZ63_RS19680 (HUZ63_19470) nadC 4064004..4064897 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUZ63_RS19685 (HUZ63_19475) pilA 4065101..4065541 (+) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  HUZ63_RS19690 (HUZ63_19480) pilB 4065551..4066936 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  HUZ63_RS19695 (HUZ63_19485) hofC 4066926..4068128 (+) 1203 WP_000157292.1 protein transport protein HofC -
  HUZ63_RS19700 (HUZ63_19490) guaC 4068163..4069206 (-) 1044 WP_001217343.1 GMP reductase -
  HUZ63_RS19705 (HUZ63_19495) - 4069362..4069406 (-) 45 WP_120795372.1 protein YacM -
  HUZ63_RS19710 (HUZ63_19500) coaE 4069431..4070051 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=402427 HUZ63_RS19685 WP_000360913.1 4065101..4065541(+) (pilA) [Escherichia coli strain STIN_95]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=402427 HUZ63_RS19685 WP_000360913.1 4065101..4065541(+) (pilA) [Escherichia coli strain STIN_95]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363