Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FOB21_RS12395 Genome accession   NZ_CP046296
Coordinates   2311013..2311813 (+) Length   266 a.a.
NCBI ID   WP_071850435.1    Uniprot ID   A0AAJ4P2Y2
Organism   Acinetobacter lwoffii strain FDAARGOS_552     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2306013..2316813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB21_RS12370 (FOB21_12370) - 2306958..2308238 (+) 1281 WP_004647459.1 glycosyltransferase family 4 protein -
  FOB21_RS12375 (FOB21_12375) - 2308259..2308810 (+) 552 WP_004278361.1 phosphatase PAP2 family protein -
  FOB21_RS12380 (FOB21_12380) iscX 2308898..2309098 (+) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  FOB21_RS12385 (FOB21_12385) ndk 2309209..2309640 (+) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  FOB21_RS12390 (FOB21_12390) rlmN 2309763..2310998 (+) 1236 WP_148334717.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FOB21_RS12395 (FOB21_12395) pilF 2311013..2311813 (+) 801 WP_071850435.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FOB21_RS12400 (FOB21_12400) - 2311804..2312595 (+) 792 WP_129716394.1 helix-turn-helix domain-containing protein -
  FOB21_RS12405 (FOB21_12405) ispG 2312619..2313740 (+) 1122 WP_004278355.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FOB21_RS12410 (FOB21_12410) hisS 2313744..2315039 (+) 1296 WP_121980028.1 histidine--tRNA ligase -
  FOB21_RS12415 (FOB21_12415) - 2315070..2315768 (+) 699 WP_004278352.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30133.26 Da        Isoelectric Point: 9.2878

>NTDB_id=402419 FOB21_RS12395 WP_071850435.1 2311013..2311813(+) (pilF) [Acinetobacter lwoffii strain FDAARGOS_552]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWIGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=402419 FOB21_RS12395 WP_071850435.1 2311013..2311813(+) (pilF) [Acinetobacter lwoffii strain FDAARGOS_552]
TTGAGAAATCTAACATCTAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATTTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCGAATATGATGATGGGCGTGCTA
TTGCAGCAGGAAGGTAGCAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCACAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGCGCACTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTACAAGCCAATCGCGATTCCTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCAGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGGCAGAAAAATCAGG
GTGCCCGTGCACTCTGGATTGGCATTCGCACTGCGCGTGCCGGAGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTCTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.846

97.744

0.771
  pilF Acinetobacter baylyi ADP1

69.925

100

0.699

Multiple sequence alignment