Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MS6192_RS00525 Genome accession   NZ_CP054940
Coordinates   117399..117839 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain MS6192     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112399..122839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS6192_RS00500 (MS6192_00105) coaE 112889..113509 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  MS6192_RS00505 - 113534..113578 (+) 45 WP_120795372.1 protein YacM -
  MS6192_RS00510 (MS6192_00106) guaC 113734..114777 (+) 1044 WP_001217338.1 GMP reductase -
  MS6192_RS00515 (MS6192_00107) hofC 114812..116014 (-) 1203 WP_000157236.1 protein transport protein HofC -
  MS6192_RS00520 (MS6192_00108) pilB 116004..117389 (-) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  MS6192_RS00525 (MS6192_00109) pilA 117399..117839 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  MS6192_RS00530 (MS6192_00110) nadC 118042..118935 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MS6192_RS00535 (MS6192_00111) ampD 119023..119574 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MS6192_RS00540 (MS6192_00112) ampE 119571..120425 (+) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  MS6192_RS00545 (MS6192_00113) aroP 120468..121841 (-) 1374 WP_001347572.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=402286 MS6192_RS00525 WP_000360895.1 117399..117839(-) (pilA) [Escherichia coli strain MS6192]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=402286 MS6192_RS00525 WP_000360895.1 117399..117839(-) (pilA) [Escherichia coli strain MS6192]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363