Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GMB29_RS00585 Genome accession   NZ_CP046266
Coordinates   113951..115324 (+) Length   457 a.a.
NCBI ID   WP_136357973.1    Uniprot ID   A0A4S4BTL4
Organism   Metabacillus sediminilitoris strain DSL-17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 108951..120324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GMB29_RS00565 (GMB29_00565) - 109158..109619 (+) 462 WP_136357982.1 CtsR family transcriptional regulator -
  GMB29_RS00570 (GMB29_00570) - 109635..110186 (+) 552 WP_136357980.1 UvrB/UvrC motif-containing protein -
  GMB29_RS00575 (GMB29_00575) - 110189..111286 (+) 1098 WP_136357978.1 protein arginine kinase -
  GMB29_RS00580 (GMB29_00580) clpC 111283..113721 (+) 2439 WP_136357975.1 ATP-dependent protease ATP-binding subunit ClpC -
  GMB29_RS00585 (GMB29_00585) radA 113951..115324 (+) 1374 WP_136357973.1 DNA repair protein RadA Machinery gene
  GMB29_RS00590 (GMB29_00590) disA 115328..116407 (+) 1080 WP_136357971.1 DNA integrity scanning diadenylate cyclase DisA -
  GMB29_RS00595 (GMB29_00595) - 116598..117695 (+) 1098 WP_136357969.1 PIN/TRAM domain-containing protein -
  GMB29_RS00600 (GMB29_00600) ispD 118110..118781 (+) 672 WP_136357967.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GMB29_RS00605 (GMB29_00605) ispF 118859..119338 (+) 480 WP_136357965.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49652.22 Da        Isoelectric Point: 8.1532

>NTDB_id=402072 GMB29_RS00585 WP_136357973.1 113951..115324(+) (radA) [Metabacillus sediminilitoris strain DSL-17]
MAKTKVKFICQSCGYESPKWMGKCPGCGEWNKMTEEILKPVSPRKATFAHSNQTVQKPSPITNIETTQEPRIFTNLKEFN
RVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSSQLADNGHDVLYISGEESVKQTKLRADRLGVKADKLFVLSETDMSYISQ
AIEETNPSFVVVDSIQTVFHSELSSAPGSVSQVRESTADLMRIAKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLYFE
GERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVKNPSEIFLEERSKGAAGSTVVASMEGTRPVLVEIQALISPTSFG
NPRRMATGIDHNRVPLLMAVLEKRVGLLLQNQDAYLKVAGGIKLDEPAIDLAVAVSIASSFKDAPPKPTDVIIGEVGLTG
EVRRVSRIEQRVIEAAKLGFKRAIIPEANIGGWSPPDNIEIVGIKNVAEALHKTLGG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=402072 GMB29_RS00585 WP_136357973.1 113951..115324(+) (radA) [Metabacillus sediminilitoris strain DSL-17]
ATGGCTAAAACAAAGGTGAAATTTATTTGTCAATCATGTGGATACGAATCACCGAAATGGATGGGTAAATGCCCAGGTTG
TGGAGAATGGAATAAAATGACAGAGGAGATTTTGAAGCCTGTATCGCCAAGAAAGGCTACTTTTGCTCATTCCAACCAAA
CCGTTCAAAAACCTTCCCCGATTACAAATATCGAAACAACCCAAGAACCAAGGATTTTTACAAATTTAAAAGAATTTAAC
CGTGTATTAGGAAGCGGTATTGTTAAAGGGTCTCTCGTGTTAATTGGTGGTGATCCTGGTATTGGTAAGTCTACGCTTCT
GCTGCAAGTATCCTCCCAATTAGCAGATAATGGTCACGATGTCTTATATATATCCGGAGAAGAATCTGTTAAACAAACGA
AATTAAGAGCAGATCGCTTAGGAGTAAAAGCAGATAAACTATTCGTCTTATCAGAAACAGATATGAGTTATATTTCTCAG
GCAATTGAGGAAACAAACCCTTCATTTGTTGTTGTTGATTCCATTCAAACGGTATTCCATAGTGAATTAAGCTCAGCTCC
AGGAAGTGTATCACAAGTAAGAGAATCAACAGCAGATCTAATGAGAATTGCTAAAACAAAAGGTATCGCTATTTTTATTG
TCGGTCATGTTACAAAGGAAGGATCAATTGCTGGTCCGCGTTTATTAGAGCACATGGTAGATACTGTACTCTACTTTGAA
GGAGAAAGACATCATACATACCGCATTTTACGAGCCGTGAAGAATCGTTTTGGATCAACGAATGAAATGGGTATTTTTGA
GATGAAGGAATCAGGTTTAGAAGAAGTAAAAAATCCTTCAGAAATATTTTTAGAAGAAAGATCGAAAGGTGCTGCTGGAT
CAACGGTAGTAGCCTCAATGGAGGGAACTAGACCGGTTCTTGTTGAAATTCAGGCATTAATTTCACCTACAAGCTTTGGT
AATCCAAGGAGAATGGCAACAGGTATCGATCATAATCGTGTCCCATTATTAATGGCAGTGTTAGAGAAACGTGTAGGACT
CCTTCTGCAAAATCAAGATGCCTATTTGAAGGTTGCGGGTGGAATTAAACTTGATGAACCAGCTATCGATTTAGCTGTTG
CTGTAAGTATTGCTTCAAGCTTTAAAGATGCACCACCAAAACCAACAGATGTCATAATTGGTGAAGTTGGTTTAACTGGA
GAGGTTAGAAGAGTTTCGAGGATTGAGCAAAGGGTCATAGAAGCTGCAAAGCTTGGATTTAAGCGGGCGATTATTCCTGA
AGCAAATATTGGTGGGTGGAGTCCTCCGGATAACATTGAGATCGTTGGGATAAAAAATGTAGCTGAGGCCCTCCATAAAA
CGTTAGGAGGATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4S4BTL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

80.786

100

0.81
  radA Streptococcus mitis NCTC 12261

60

99.562

0.597
  radA Streptococcus mitis SK321

60

99.562

0.597
  radA Streptococcus pneumoniae Rx1

59.78

99.562

0.595
  radA Streptococcus pneumoniae D39

59.78

99.562

0.595
  radA Streptococcus pneumoniae R6

59.78

99.562

0.595
  radA Streptococcus pneumoniae TIGR4

59.78

99.562

0.595

Multiple sequence alignment