Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GL174_RS02370 Genome accession   NZ_CP046252
Coordinates   472895..474262 (+) Length   455 a.a.
NCBI ID   WP_155178870.1    Uniprot ID   A0A6I5YK05
Organism   Sphingobium sp. CAP-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 467895..479262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GL174_RS02345 (GL174_02345) - 468409..468827 (-) 419 Protein_466 metallopeptidase family protein -
  GL174_RS02350 (GL174_02350) - 468856..469146 (+) 291 WP_155178864.1 4a-hydroxytetrahydrobiopterin dehydratase -
  GL174_RS02355 (GL174_02355) - 469217..469723 (-) 507 WP_230461260.1 hypothetical protein -
  GL174_RS02360 (GL174_02360) - 469720..472065 (-) 2346 WP_155178866.1 patatin-like protein -
  GL174_RS02365 (GL174_02365) - 472108..472806 (-) 699 WP_155178868.1 endonuclease/exonuclease/phosphatase family protein -
  GL174_RS02370 (GL174_02370) radA 472895..474262 (+) 1368 WP_155178870.1 DNA repair protein RadA Machinery gene
  GL174_RS02375 (GL174_02375) - 474274..474795 (+) 522 WP_155178872.1 CvpA family protein -
  GL174_RS02380 (GL174_02380) - 474799..475320 (+) 522 WP_230461261.1 hypothetical protein -
  GL174_RS02385 (GL174_02385) - 475340..476269 (-) 930 WP_196221742.1 NAD(P)H-binding protein -
  GL174_RS02390 (GL174_02390) proB 476272..477402 (-) 1131 WP_155178874.1 glutamate 5-kinase -
  GL174_RS02395 (GL174_02395) obgE 477551..478648 (-) 1098 WP_155178875.1 GTPase ObgE -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 47893.81 Da        Isoelectric Point: 6.3964

>NTDB_id=402003 GL174_RS02370 WP_155178870.1 472895..474262(+) (radA) [Sphingobium sp. CAP-1]
MAKAKRKFVCQQCGTVTSRWQGQCEDCGEWNSIVEEAAETVFSARHDLQNGGRAITLVGLDSDVELPPRTSTGIAEFDRA
LGGGIVHGSATLIGGDPGIGKSTLLLQAAARIASRGLSVAYISGEEAADQVRLRAQRLGLGNAPVQLASATSVRDILTTL
SEGPPPALLVIDSIQTMHSDLIEGAPGTVSQVRASSQELIKFAKQRGTALILVGHVTKDGSIAGPRVLEHMVDTVLAFEG
ERSHQYRILRAVKNRFGGTDEIGVFAMVAEGLEEVANPSALFLTNRDETVTGATVFPALEGTRPVLVEIQALVVRLSSGA
TPRRAVVGWDSGRLAMILAVLEARCGLSFSTCEVYLNVAGGYRLSDPAADLAVAAALISAMSERPVPVDVVLFGEIALSS
EIRPVSHSPLRLREAAKLGFTRAFVPASAADGVKGIAVNGFRTLAQLVDQMLGRG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=402003 GL174_RS02370 WP_155178870.1 472895..474262(+) (radA) [Sphingobium sp. CAP-1]
ATGGCCAAGGCAAAACGCAAATTCGTCTGTCAGCAATGCGGCACCGTGACCAGCCGGTGGCAGGGCCAGTGCGAGGATTG
CGGCGAATGGAACAGCATCGTCGAGGAAGCTGCTGAAACCGTCTTTTCCGCGCGCCATGACCTGCAAAATGGTGGCCGCG
CCATCACTTTGGTCGGCCTCGACAGCGACGTGGAACTGCCCCCGCGCACCAGCACCGGTATCGCCGAGTTCGACCGGGCG
CTGGGCGGCGGCATCGTTCATGGCTCCGCGACGCTGATCGGCGGCGATCCGGGCATCGGCAAATCGACCCTGCTGTTGCA
AGCGGCGGCGCGCATCGCCTCGCGCGGCCTGTCGGTCGCCTATATCAGCGGCGAGGAAGCCGCCGATCAGGTCCGCCTGC
GCGCCCAGCGGCTTGGCCTTGGCAATGCGCCGGTGCAACTCGCCAGCGCCACCTCCGTCCGCGACATCCTGACCACGCTC
AGCGAAGGCCCGCCGCCCGCCCTGCTCGTCATCGATTCGATCCAGACGATGCACAGCGACCTGATCGAAGGGGCGCCCGG
CACCGTCAGCCAGGTCCGCGCCTCCAGCCAGGAACTCATCAAATTCGCCAAGCAGCGCGGCACGGCGCTCATCCTCGTCG
GCCATGTGACCAAGGATGGCAGCATCGCCGGCCCGCGCGTGCTGGAACATATGGTCGACACGGTGCTGGCGTTCGAGGGC
GAGCGCAGCCATCAATATCGCATCCTGCGTGCGGTCAAGAACCGCTTCGGCGGCACTGACGAGATCGGCGTGTTCGCCAT
GGTGGCGGAGGGACTGGAGGAGGTCGCCAACCCCTCCGCCTTGTTTCTCACCAACCGCGACGAAACGGTGACGGGCGCGA
CCGTCTTTCCCGCGCTGGAAGGGACTCGGCCGGTGCTGGTCGAAATTCAGGCGCTGGTGGTGCGCCTCTCCAGCGGCGCG
ACACCCCGGCGCGCGGTGGTCGGCTGGGACAGCGGACGGCTCGCCATGATCCTCGCCGTGCTGGAGGCGCGCTGCGGCCT
CAGCTTCTCGACCTGCGAAGTCTATTTGAACGTTGCGGGCGGCTATCGCCTGTCGGACCCGGCCGCCGACCTTGCCGTCG
CCGCCGCACTCATTTCCGCCATGTCGGAACGGCCGGTGCCGGTCGATGTCGTGCTGTTCGGCGAAATCGCGCTGTCGAGC
GAAATCCGTCCCGTCTCCCATTCGCCCCTGCGTTTGCGCGAAGCCGCGAAGCTGGGCTTCACCCGCGCCTTCGTACCCGC
GTCGGCCGCGGACGGGGTAAAGGGAATCGCGGTTAATGGTTTCCGCACCCTCGCGCAGTTGGTTGACCAGATGCTGGGAC
GCGGATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(82-220)

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I5YK05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

47.939

100

0.486
  radA Streptococcus mitis NCTC 12261

46.272

100

0.464
  radA Streptococcus pneumoniae Rx1

46.053

100

0.462
  radA Streptococcus pneumoniae D39

46.053

100

0.462
  radA Streptococcus pneumoniae R6

46.053

100

0.462
  radA Streptococcus pneumoniae TIGR4

46.053

100

0.462
  radA Streptococcus mitis SK321

45.833

100

0.459

Multiple sequence alignment