Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   HUS13_RS30805 Genome accession   NZ_CP054845
Coordinates   6489194..6489724 (-) Length   176 a.a.
NCBI ID   WP_202096831.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5429     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6484194..6494724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUS13_RS30770 (HUS13_30760) - 6485371..6485652 (+) 282 Protein_6110 hypothetical protein -
  HUS13_RS34010 - 6485858..6486025 (+) 168 WP_242532313.1 hypothetical protein -
  HUS13_RS30780 (HUS13_30770) - 6486282..6486745 (+) 464 Protein_6112 DUF3164 family protein -
  HUS13_RS30785 (HUS13_30775) - 6486833..6487798 (+) 966 WP_242532314.1 DUF2786 domain-containing protein -
  HUS13_RS30790 (HUS13_30780) - 6487744..6488211 (+) 468 WP_003129239.1 hypothetical protein -
  HUS13_RS30795 (HUS13_30785) - 6488198..6488761 (+) 564 WP_023657063.1 regulatory protein GemA -
  HUS13_RS30800 (HUS13_30790) - 6488764..6489123 (+) 360 WP_003094179.1 Mor transcription activator family protein -
  HUS13_RS30805 (HUS13_30795) pilN 6489194..6489724 (-) 531 WP_202096831.1 PilN domain-containing protein Machinery gene
  HUS13_RS30810 (HUS13_30800) pilM 6489724..6490788 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HUS13_RS30815 (HUS13_30805) - 6490973..6493444 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 176 a.a.        Molecular weight: 20039.24 Da        Isoelectric Point: 10.2416

>NTDB_id=401879 HUS13_RS30805 WP_202096831.1 6489194..6489724(-) (pilN) [Pseudomonas aeruginosa strain SE5429]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKV
REGEKYLGGGARKIEI

Nucleotide


Download         Length: 531 bp        

>NTDB_id=401879 HUS13_RS30805 WP_202096831.1 6489194..6489724(-) (pilN) [Pseudomonas aeruginosa strain SE5429]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCGGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGATTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGTCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATTGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGTG
AGAGAGGGTGAGAAATACCTAGGGGGAGGTGCGCGAAAAATCGAGATATAG

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

44.025

90.341

0.398
  pilN Acinetobacter baumannii D1279779

42.424

93.75

0.398
  comN/pilN Acinetobacter baylyi ADP1

40.719

94.886

0.386