Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GKP14_RS08175 Genome accession   NZ_CP046161
Coordinates   1700484..1701875 (+) Length   463 a.a.
NCBI ID   WP_086035708.1    Uniprot ID   A0A859DUK5
Organism   Caproicibacterium lactatifermentans strain JNU-WLY1368     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1695484..1706875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKP14_RS08125 (GKP14_08115) - 1695820..1696149 (+) 330 WP_086035703.1 YerC/YecD family TrpR-related protein -
  GKP14_RS08130 (GKP14_08120) hslO 1696226..1697119 (+) 894 WP_274380231.1 Hsp33 family molecular chaperone HslO -
  GKP14_RS08160 (GKP14_08150) - 1697679..1698212 (+) 534 WP_086035705.1 DNA-deoxyinosine glycosylase -
  GKP14_RS08165 (GKP14_08155) - 1698196..1699578 (+) 1383 WP_086035706.1 MATE family efflux transporter -
  GKP14_RS08170 (GKP14_08160) - 1699637..1700419 (+) 783 WP_157658930.1 N-acetylmuramoyl-L-alanine amidase -
  GKP14_RS08175 (GKP14_08165) radA 1700484..1701875 (+) 1392 WP_086035708.1 DNA repair protein RadA Machinery gene
  GKP14_RS08180 (GKP14_08170) - 1702081..1702875 (+) 795 WP_086035709.1 DUF6873 family GME fold protein -
  GKP14_RS08185 (GKP14_08175) - 1702942..1704048 (+) 1107 WP_086035710.1 tyrosine recombinase XerC -
  GKP14_RS08190 (GKP14_08180) - 1704131..1705096 (-) 966 WP_157658931.1 YesL family protein -
  GKP14_RS08195 (GKP14_08185) xerD 1705129..1706022 (-) 894 WP_086035712.1 site-specific tyrosine recombinase XerD -
  GKP14_RS08200 (GKP14_08190) - 1706039..1706680 (-) 642 WP_086035713.1 stage II sporulation protein M -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 50913.41 Da        Isoelectric Point: 6.1857

>NTDB_id=401722 GKP14_RS08175 WP_086035708.1 1700484..1701875(+) (radA) [Caproicibacterium lactatifermentans strain JNU-WLY1368]
MAGKLKSIYRCTECGYESAKWYGKCPECGAWNTMSEALQEPAVSMRRGSIGQGIYHQTAPQATPIREISMEEEPRYKTGV
SELDRVLGGGIVKGSIILISGEPGIGKSTILTQICAYLGNTLKILYVSGEESSRQIKLRANRLGVEPDNLYILTETDIQR
VLEQVRTEKPDLLMIDSIQTMNDTELNSAPGSVTQVRECTNAIMHIAKGMEIPAIMVGHVNKDGAIAGPKVLEHIVDAVL
YFEGDRQMSYRILRAVKNRYGSTNEIGVFDMEETGLHQVENPSASMLTGRPTNVSGTCVTCVMEGSRPILAELQALVTSS
GFGNPRRMATGFDYNRMNLLLAVLEKRAGYYFSNLDAYLNVVGGLRLDEPACDLAVSMALVSSLKDVPIADDAVIFGEVG
LTGEVRSVMHAEQRIREAERMGFHRCILPWYNLKYLSGRQANTTIQLTGVKNVRQAFAALQQE

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=401722 GKP14_RS08175 WP_086035708.1 1700484..1701875(+) (radA) [Caproicibacterium lactatifermentans strain JNU-WLY1368]
ATGGCAGGAAAACTAAAAAGTATTTATAGATGTACAGAATGCGGATATGAATCTGCAAAATGGTATGGAAAATGTCCGGA
ATGCGGTGCATGGAATACCATGTCTGAAGCGCTGCAGGAGCCAGCTGTTTCGATGCGGCGCGGTAGTATAGGGCAGGGAA
TTTATCATCAAACAGCGCCGCAGGCGACACCAATTCGAGAAATTAGCATGGAGGAGGAGCCACGCTATAAAACGGGCGTT
TCGGAACTGGACCGCGTTCTAGGTGGTGGAATTGTAAAAGGTTCCATCATCTTAATTAGTGGTGAACCGGGAATCGGTAA
GTCAACGATATTAACGCAGATTTGCGCTTATTTAGGAAATACTCTGAAAATTCTATATGTTTCTGGTGAGGAGTCCAGCC
GACAGATTAAGCTAAGGGCAAACCGATTGGGAGTGGAGCCAGACAATCTTTATATTTTGACGGAAACAGATATACAAAGA
GTTTTGGAGCAGGTCCGAACGGAAAAACCGGATTTATTGATGATTGACTCTATTCAAACGATGAATGATACAGAATTAAA
CTCCGCTCCCGGCAGCGTTACACAGGTTCGTGAATGCACAAATGCGATTATGCATATCGCAAAAGGAATGGAGATTCCTG
CAATCATGGTAGGTCATGTCAACAAAGACGGAGCCATTGCTGGGCCGAAGGTCTTGGAACATATTGTTGATGCGGTTTTA
TATTTTGAAGGTGACCGTCAGATGAGCTACCGCATCTTGCGCGCGGTAAAAAACCGGTATGGCTCCACCAATGAAATTGG
TGTTTTCGATATGGAAGAAACGGGGCTGCATCAAGTAGAAAACCCGTCAGCATCTATGCTGACGGGTCGGCCAACGAATG
TTTCCGGTACTTGTGTTACCTGTGTGATGGAAGGCAGCCGTCCTATATTGGCGGAGCTGCAGGCACTGGTCACTTCGTCC
GGCTTTGGGAATCCCAGAAGAATGGCAACCGGCTTTGACTATAACCGCATGAATTTACTCCTTGCGGTGCTGGAAAAGCG
GGCGGGCTATTATTTTTCAAATCTTGATGCCTATTTAAATGTAGTAGGCGGTCTGCGTTTGGACGAACCTGCGTGTGATT
TGGCGGTATCCATGGCGCTGGTTTCCAGCCTGAAGGATGTACCGATTGCCGATGATGCGGTCATTTTTGGAGAAGTGGGC
TTAACGGGAGAGGTTCGTTCAGTTATGCATGCAGAACAGCGTATCAGAGAAGCAGAACGAATGGGTTTTCATCGATGCAT
TCTTCCGTGGTACAATTTAAAGTATCTTAGCGGCCGGCAGGCAAATACAACGATACAGCTGACCGGCGTTAAAAATGTGA
GACAGGCTTTTGCGGCGCTTCAGCAGGAGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(352-430)

AAA domain (AAA_25)

(80-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A859DUK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

52.747

98.272

0.518
  radA Streptococcus pneumoniae Rx1

47.807

98.488

0.471
  radA Streptococcus pneumoniae D39

47.807

98.488

0.471
  radA Streptococcus pneumoniae R6

47.807

98.488

0.471
  radA Streptococcus pneumoniae TIGR4

47.807

98.488

0.471
  radA Streptococcus mitis SK321

47.807

98.488

0.471
  radA Streptococcus mitis NCTC 12261

47.807

98.488

0.471

Multiple sequence alignment