Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   GKO36_RS12955 Genome accession   NZ_CP046145
Coordinates   2645519..2646319 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain PEBA20     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2640519..2651319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKO36_RS12925 (GKO36_12840) - 2641066..2641698 (+) 633 WP_014418348.1 helix-turn-helix domain-containing protein -
  GKO36_RS12930 - 2641989..2642126 (+) 138 WP_014470646.1 hypothetical protein -
  GKO36_RS12935 (GKO36_12845) - 2642220..2643338 (+) 1119 WP_032863662.1 NADH-dependent flavin oxidoreductase -
  GKO36_RS12940 (GKO36_12850) - 2643465..2643677 (+) 213 WP_029973891.1 spore germination protein -
  GKO36_RS12945 (GKO36_12855) - 2643802..2644014 (+) 213 WP_014418351.1 spore germination protein -
  GKO36_RS12950 (GKO36_12860) alr 2644191..2645372 (+) 1182 WP_007408361.1 alanine racemase -
  GKO36_RS12955 (GKO36_12865) spo0A 2645519..2646319 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  GKO36_RS12960 (GKO36_12870) spoIVB 2646600..2647880 (-) 1281 WP_014418352.1 SpoIVB peptidase -
  GKO36_RS12965 (GKO36_12875) recN 2648044..2649774 (-) 1731 WP_007408359.1 DNA repair protein RecN Machinery gene
  GKO36_RS12970 (GKO36_12880) argR 2649806..2650255 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  GKO36_RS12975 (GKO36_12885) - 2650352..2651197 (-) 846 WP_014418353.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=401629 GKO36_RS12955 WP_003153177.1 2645519..2646319(-) (spo0A) [Bacillus velezensis strain PEBA20]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=401629 GKO36_RS12955 WP_003153177.1 2645519..2646319(-) (spo0A) [Bacillus velezensis strain PEBA20]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCTGATGTACTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATCTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAATTCAGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment