Detailed information    

insolico Bioinformatically predicted

Overview

Name   rok   Type   Regulator
Locus tag   GKO36_RS07025 Genome accession   NZ_CP046145
Coordinates   1362349..1362945 (+) Length   198 a.a.
NCBI ID   WP_014417618.1    Uniprot ID   I2C4E7
Organism   Bacillus velezensis strain PEBA20     
Function   repression of comK (predicted from homology)   
Competence regulation

Genomic Context

Location: 1357349..1367945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKO36_RS06995 (GKO36_06995) - 1357563..1358018 (+) 456 WP_014417614.1 flavodoxin -
  GKO36_RS07000 (GKO36_07000) dapD 1358100..1358810 (+) 711 WP_007409623.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase -
  GKO36_RS07005 (GKO36_07005) - 1358881..1360005 (+) 1125 WP_014417615.1 N-acetyldiaminopimelate deacetylase -
  GKO36_RS07010 (GKO36_07010) - 1360066..1360311 (+) 246 WP_003154596.1 YkuS family protein -
  GKO36_RS07015 (GKO36_07015) - 1360345..1361151 (-) 807 WP_014417616.1 mechanosensitive ion channel family protein -
  GKO36_RS07020 (GKO36_07020) - 1361439..1361897 (+) 459 WP_014417617.1 TlpA disulfide reductase family protein -
  GKO36_RS07025 (GKO36_07025) rok 1362349..1362945 (+) 597 WP_014417618.1 transcriptional regulator Rok Regulator
  GKO36_RS07030 (GKO36_07030) - 1362966..1363958 (-) 993 WP_014417619.1 hypothetical protein -
  GKO36_RS07035 (GKO36_07035) - 1364104..1364706 (+) 603 WP_014417620.1 molybdenum cofactor guanylyltransferase -
  GKO36_RS07040 (GKO36_07040) - 1364758..1365777 (+) 1020 WP_007611113.1 molybdopterin-synthase adenylyltransferase MoeB -
  GKO36_RS07045 (GKO36_07045) glp 1365795..1367087 (+) 1293 WP_014417621.1 gephyrin-like molybdotransferase Glp -
  GKO36_RS07050 (GKO36_07050) mobB 1367048..1367569 (+) 522 WP_032863763.1 molybdopterin-guanine dinucleotide biosynthesis protein B -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22529.56 Da        Isoelectric Point: 9.6909

>NTDB_id=401621 GKO36_RS07025 WP_014417618.1 1362349..1362945(+) (rok) [Bacillus velezensis strain PEBA20]
MFNEREALRLRLEQLNEAEVKVIREYQIERDKIYAKLRELDNTGSLSDTKKEFRPEKKSDSLPVLAELAAQEMRSYQKPQ
TQTQSSQPQMQSITSLPAGIPDGSTRRRRGTARPGSKAAKLREAAIKTLKRHNAAIKSSELQKEIEKESGLEIPNMTTFM
QSLIKMYPEVKKPYRGQYILEGEISQETAETANDTAAE

Nucleotide


Download         Length: 597 bp        

>NTDB_id=401621 GKO36_RS07025 WP_014417618.1 1362349..1362945(+) (rok) [Bacillus velezensis strain PEBA20]
ATGTTTAATGAAAGAGAAGCGCTGCGCTTACGTTTAGAACAATTGAACGAAGCAGAGGTAAAAGTCATTCGTGAATATCA
AATAGAACGTGATAAAATATATGCAAAACTGAGAGAATTAGACAATACAGGCAGCCTGTCCGATACAAAAAAAGAGTTTC
GTCCGGAGAAAAAAAGTGACTCTCTCCCGGTGCTTGCAGAGCTTGCGGCACAGGAAATGAGAAGCTATCAAAAGCCGCAG
ACACAGACTCAATCCTCCCAGCCGCAGATGCAGTCTATTACGTCTCTTCCCGCCGGCATTCCGGACGGTTCTACAAGAAG
AAGAAGAGGGACGGCGAGACCGGGATCAAAAGCGGCAAAACTGCGCGAAGCAGCGATTAAAACGTTGAAACGCCACAATG
CAGCGATCAAAAGTTCCGAACTGCAAAAGGAAATTGAAAAGGAAAGCGGCCTTGAAATTCCTAATATGACAACATTTATG
CAAAGCTTAATTAAAATGTATCCGGAAGTGAAAAAACCATACCGCGGCCAATATATTCTTGAGGGAGAAATTTCTCAGGA
AACAGCAGAAACGGCAAATGATACGGCTGCTGAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C4E7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rok Bacillus subtilis subsp. subtilis str. 168

86.082

97.98

0.843

Multiple sequence alignment