Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GKO36_RS01750 Genome accession   NZ_CP046145
Coordinates   331249..331686 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain PEBA20     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 326249..336686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKO36_RS01725 (GKO36_01730) - 327269..328405 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  GKO36_RS01730 (GKO36_01735) - 328420..328854 (+) 435 WP_014416871.1 RDD family protein -
  GKO36_RS01735 (GKO36_01740) - 328926..329249 (+) 324 WP_007409357.1 YckD family protein -
  GKO36_RS01740 (GKO36_01745) - 329353..330789 (+) 1437 WP_014416872.1 family 1 glycosylhydrolase -
  GKO36_RS01745 (GKO36_01750) nin/comJ 330830..331228 (-) 399 WP_014416873.1 competence protein ComJ Regulator
  GKO36_RS01750 (GKO36_01755) nucA/comI 331249..331686 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GKO36_RS01755 (GKO36_01760) hxlB 332017..332574 (-) 558 WP_014416874.1 6-phospho-3-hexuloisomerase -
  GKO36_RS01760 (GKO36_01765) hxlA 332571..333206 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  GKO36_RS01765 (GKO36_01770) - 333438..333800 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=401599 GKO36_RS01750 WP_003156588.1 331249..331686(-) (nucA/comI) [Bacillus velezensis strain PEBA20]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=401599 GKO36_RS01750 WP_003156588.1 331249..331686(-) (nucA/comI) [Bacillus velezensis strain PEBA20]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment