Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUK78_RS28690 Genome accession   NZ_CP054794
Coordinates   6153969..6155753 (+) Length   594 a.a.
NCBI ID   WP_031631428.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain A0002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6148969..6160753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK78_RS28670 (HUK78_28660) argE 6149207..6150361 (-) 1155 WP_003457770.1 acetylornithine deacetylase -
  HUK78_RS28675 (HUK78_28665) - 6150461..6151729 (-) 1269 WP_023107373.1 inorganic phosphate transporter -
  HUK78_RS28680 (HUK78_28670) - 6151776..6152453 (-) 678 WP_003096272.1 TIGR00153 family protein -
  HUK78_RS28685 (HUK78_28675) - 6152567..6153931 (+) 1365 WP_003141930.1 inorganic triphosphatase -
  HUK78_RS28690 (HUK78_28680) pilB 6153969..6155753 (+) 1785 WP_031631428.1 GspE/PulE family protein Machinery gene
  HUK78_RS28695 (HUK78_28685) - 6155975..6156427 (+) 453 WP_003107402.1 NfeD family protein -
  HUK78_RS28700 (HUK78_28690) - 6156559..6156888 (+) 330 WP_003096280.1 DUF2388 domain-containing protein -
  HUK78_RS28705 (HUK78_28695) gcvP 6156923..6159799 (-) 2877 WP_096314363.1 aminomethyl-transferring glycine dehydrogenase -
  HUK78_RS28710 (HUK78_28700) gcvH 6159973..6160362 (-) 390 WP_003107397.1 glycine cleavage system protein GcvH -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 66011.63 Da        Isoelectric Point: 6.5096

>NTDB_id=401583 HUK78_RS28690 WP_031631428.1 6153969..6155753(+) (pilB) [Pseudomonas aeruginosa strain A0002]
MSVFATAAQDRLLDLGDLLRELVTQGRVGQEQAEQCLAIRRSAVRNQQHPLEFLAAQQVEDLKRPGRKLDLETLTQWLAE
YAGQPYLRIDPLKIDVAAVTPLMSYAFAQRHGILAVALSPEEVTIASAQPFVQGWESNLVHVLKRPIKRVVANPADIQRY
TVEFFRLARSVSGATSPDQKISGVGNFEQLLNLGAADQEPDANDAHIVTIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHNVYQFPPQVTMAVVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGAEVELRLSTLPTAFGEKMVMRIFDPEVLLKS
FDQLGFSADDLRRWQSMTSQPNGIILVTGPTGSGKTTTLYTTLKQLATDEVNVCTIEDPIEMIEPSFNQMQVQHNIDLTF
ASGVRALLRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDSPTAITRLLELGVPYYLIRATVLGVMAQRLVRT
LCPHCKAPMQLADSDWHELTRPWSAPPPSGAHQAVGCLECRDTGYRGRAGVYEIMLLSDSLKELITADTDLVALRRQAYK
EGTRSLRLSGAQKVAAGISTLEEVLRVTPQSEQK

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=401583 HUK78_RS28690 WP_031631428.1 6153969..6155753(+) (pilB) [Pseudomonas aeruginosa strain A0002]
ATGTCCGTGTTCGCCACCGCCGCCCAGGACCGCCTGCTGGACCTCGGCGACCTGCTCCGCGAGCTGGTCACCCAGGGCCG
GGTCGGGCAGGAGCAGGCCGAACAGTGCCTGGCGATCCGCCGCAGCGCGGTGCGCAACCAGCAGCATCCGCTGGAGTTCC
TCGCCGCGCAGCAGGTCGAGGATCTCAAGCGCCCCGGACGCAAGCTCGACCTGGAGACCCTCACCCAGTGGCTGGCCGAG
TACGCCGGCCAGCCCTACCTGCGCATCGACCCGCTGAAGATCGACGTCGCCGCGGTGACTCCGCTGATGTCCTACGCCTT
CGCCCAGCGCCATGGCATCCTCGCCGTCGCGCTGAGTCCGGAGGAAGTCACCATCGCCAGCGCCCAGCCCTTCGTACAGG
GCTGGGAAAGCAACCTGGTGCATGTGCTCAAGCGACCGATCAAGCGGGTGGTGGCGAACCCGGCGGACATCCAGCGCTAC
ACCGTCGAGTTCTTCCGCCTGGCCCGCTCGGTCAGCGGCGCCACCTCGCCGGACCAGAAGATCAGCGGCGTCGGCAACTT
CGAGCAGTTGCTCAACCTCGGCGCCGCCGACCAGGAGCCGGACGCCAACGACGCGCACATCGTCACCATCGTCGACTGGC
TGTTCCAGTACGCCTTCCAGCAGCGCGCCAGCGACATCCACATCGAGCCGCGCCGCGAGCAGGGCACCGTGCGCTTCCGT
ATCGACGGCGTGCTGCACAACGTCTACCAGTTCCCGCCGCAGGTGACCATGGCGGTGGTCAGCCGCCTGAAGTCGCTGGG
ACGGATGAACGTGGCGGAGAAACGCAAGCCGCAGGACGGCCGGGTCAAGACCAAGACCCCGGACGGCGCCGAGGTGGAGC
TGCGCCTGTCGACCCTGCCCACCGCCTTCGGCGAGAAGATGGTGATGCGCATCTTCGACCCCGAGGTGCTGCTCAAGAGC
TTCGACCAGCTCGGCTTCTCCGCCGACGACCTGCGCCGCTGGCAGAGCATGACCAGCCAGCCCAACGGCATCATCCTGGT
CACCGGCCCCACCGGCTCGGGCAAGACCACCACGCTCTACACCACGCTCAAGCAACTGGCCACCGATGAGGTGAACGTCT
GCACCATCGAGGACCCGATCGAGATGATCGAGCCCTCGTTCAACCAGATGCAGGTCCAGCACAACATCGACCTGACCTTC
GCCAGCGGCGTACGCGCCCTGCTGCGGCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGA
GATGGCGATCCAGGCGGCGCTCACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGACTCCCCCACGGCGATCACCC
GCCTGCTCGAACTGGGCGTGCCCTACTACCTGATCCGCGCCACGGTGCTAGGGGTCATGGCCCAGCGCCTGGTGCGCACG
CTCTGCCCGCACTGCAAGGCGCCGATGCAACTGGCGGACAGCGACTGGCACGAACTGACCCGCCCCTGGAGCGCCCCGCC
GCCCAGCGGCGCGCACCAGGCCGTGGGTTGCCTGGAGTGCCGCGACACCGGCTATCGCGGGCGCGCCGGCGTCTATGAAA
TCATGCTGCTCAGCGATAGCCTGAAAGAACTGATCACCGCCGACACCGACCTGGTCGCCTTGCGCCGCCAGGCGTACAAG
GAAGGCACCCGCAGCCTGCGCCTGTCCGGCGCGCAGAAGGTCGCCGCAGGTATCAGTACGCTGGAGGAAGTACTGCGGGT
GACCCCGCAGAGCGAACAGAAGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(216-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.682

90.067

0.375

Multiple sequence alignment