Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GKC13_RS01050 Genome accession   NZ_CP046134
Coordinates   181227..182588 (+) Length   453 a.a.
NCBI ID   WP_207560113.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain MAG_rmk202_sterm     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 176227..187588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC13_RS01025 (GKC13_01020) - 177029..177601 (-) 573 WP_179970814.1 MptD family putative ECF transporter S component -
  GKC13_RS01030 (GKC13_01025) - 177610..178400 (-) 791 Protein_197 formate/nitrite transporter family protein -
  GKC13_RS01035 (GKC13_01030) - 178518..179768 (-) 1251 WP_179970815.1 NAD(P)H-dependent oxidoreductase -
  GKC13_RS01040 (GKC13_01035) - 179787..180389 (-) 603 Protein_199 NADPH-dependent FMN reductase -
  GKC13_RS01045 (GKC13_01040) - 180603..181049 (+) 447 WP_002947069.1 dUTP diphosphatase -
  GKC13_RS01050 (GKC13_01045) radA 181227..182588 (+) 1362 WP_207560113.1 DNA repair protein RadA Machinery gene
  GKC13_RS01055 (GKC13_01050) - 182677..183373 (+) 697 Protein_202 TIGR00266 family protein -
  GKC13_RS01060 (GKC13_01055) - 183516..184013 (+) 498 WP_002947059.1 carbonic anhydrase -
  GKC13_RS01065 (GKC13_01060) - 184156..185726 (+) 1571 WP_087009847.1 IS3-like element ISSth1b family transposase -
  GKC13_RS01070 (GKC13_01065) - 185792..186145 (-) 354 WP_002953536.1 DUF3397 domain-containing protein -
  GKC13_RS01075 (GKC13_01070) rplK 186317..186742 (+) 426 WP_002953535.1 50S ribosomal protein L11 -
  GKC13_RS01080 (GKC13_01075) rplA 186842..187531 (+) 690 WP_002946412.1 50S ribosomal protein L1 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49404.78 Da        Isoelectric Point: 6.6528

>NTDB_id=401498 GKC13_RS01050 WP_207560113.1 181227..182588(+) (radA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
MAKKKSTFVCRECGYNSPKYLGRCPNCSSWSSFEEEVEVQEVKNARVSLTGEKSKPTKLKEVSSISYARIKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAVRSQVE
GIKPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELIQIAKTNNIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEVGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGSRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKELPTNPQEAFVGEIGLTGEIR
RVTRIEQRINEAAKLGFTKIYVPKNSLHGMKIPEGIQVIGVTTVGEVLKKVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=401498 GKC13_RS01050 WP_207560113.1 181227..182588(+) (radA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
ATAGCTAAGAAAAAATCAACTTTTGTTTGTAGAGAATGTGGCTACAATTCACCCAAATATCTAGGGCGCTGTCCCAATTG
TTCTTCTTGGTCCTCTTTTGAGGAAGAGGTTGAGGTGCAGGAGGTTAAAAATGCCCGTGTCAGTTTGACGGGTGAAAAGT
CAAAACCTACTAAGTTAAAAGAAGTCTCTTCAATTAGTTATGCCCGCATAAAAACCGATATGGACGAGTTTAACCGTGTT
TTAGGTGGCGGTGTGGTTCCGGGGAGTTTGGTTCTTATTGGAGGAGATCCAGGAATAGGGAAATCGACCCTCCTCTTACA
GGTGTCAACACAGCTGGCTAACAAGGGAACTGTCCTCTATGTTTCTGGGGAAGAATCGGCCGAGCAAATCAAATTGCGGA
GTGAACGTTTAGGTGATATTGATAACGAATTTTATCTGTATGCTGAGACCAATATGCAAGCAGTCCGTAGTCAAGTGGAG
GGTATCAAACCCGATTTCCTCATTATTGATTCTATTCAGACCATCATGAGCCCAGAGATCTCTGGCGTTCAAGGTTCTGT
TTCCCAGGTTCGTGAAGTAACTGCCGAACTCATACAGATTGCCAAAACCAATAACATTGCCACTTTCATTGTTGGGCATG
TGACCAAGGAGGGACAGCTGGCTGGACCACGTATGTTGGAGCACATGGTGGATACTGTGCTTTATTTTGAGGGGGAACGT
CATCATACCTTCCGTATTCTGAGGGCAGTTAAAAACCGTTTTGGTTCAACCAATGAGGTTGGGATTTTTGAAATGCAGTC
AGGTGGTCTGGTCGAGGTACTAAACCCTAGCCAAGTTTTCTTAGAAGAACGCCTAGACGGTGCCACAGGTTCTGCCATTG
TTGTCACAATGGAGGGGAGTCGACCTATCCTAGCTGAGGTTCAAGCTTTGGTGACACCGACCGTCTTTGGTAACGCAAAA
CGGACAACGACGGGACTGGACTTTAACCGTGTCAGCCTTATCATGGCTGTCTTAGAGAAACGTTGCGGCCTTCTTCTCCA
AAATCAAGATGCCTATCTCAAATCAGCTGGTGGTGTCAAGTTGGACGAGCCAGCTATTGACCTTGCAGTTGCCGTTGCTA
TCGCTTCTAGTTACAAGGAGCTTCCAACCAATCCACAAGAAGCCTTCGTAGGAGAAATTGGCTTGACCGGAGAGATTCGT
CGTGTGACTCGTATTGAGCAGCGTATCAATGAAGCCGCTAAACTAGGTTTTACTAAGATTTATGTTCCTAAAAATTCGCT
TCATGGCATGAAGATTCCAGAAGGTATCCAGGTCATTGGGGTCACAACGGTAGGAGAAGTACTCAAAAAAGTCTTTTCTT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.742

100

0.887
  radA Streptococcus pneumoniae D39

88.742

100

0.887
  radA Streptococcus pneumoniae R6

88.742

100

0.887
  radA Streptococcus pneumoniae TIGR4

88.742

100

0.887
  radA Streptococcus mitis NCTC 12261

88.742

100

0.887
  radA Streptococcus mitis SK321

88.742

100

0.887
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611

Multiple sequence alignment