Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GKC17_RS02510 Genome accession   NZ_CP046131
Coordinates   479858..481234 (+) Length   458 a.a.
NCBI ID   WP_035165148.1    Uniprot ID   -
Organism   Lactobacillus delbrueckii subsp. lactis strain MAG_rmk202_ldel     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 474858..486234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC17_RS02480 (GKC17_02485) fabZ 475616..476077 (+) 462 WP_016396804.1 3-hydroxyacyl-ACP dehydratase FabZ -
  GKC17_RS02485 (GKC17_02490) - 476189..477124 (+) 936 WP_035162448.1 AAA family ATPase -
  GKC17_RS02490 (GKC17_02495) - 477118..477411 (+) 294 WP_002879759.1 hypothetical protein -
  GKC17_RS02495 (GKC17_02500) pepC 477477..478826 (-) 1350 WP_035165147.1 aminopeptidase C -
  GKC17_RS02500 (GKC17_02505) - 478911..479198 (-) 288 WP_003615858.1 hypothetical protein -
  GKC17_RS02505 (GKC17_02510) - 479304..479858 (+) 555 WP_016396797.1 dUTPase -
  GKC17_RS02510 (GKC17_02515) radA 479858..481234 (+) 1377 WP_035165148.1 DNA repair protein RadA Machinery gene
  GKC17_RS02515 (GKC17_02520) - 481344..482291 (+) 948 WP_003612526.1 glycosyltransferase family 8 protein -
  GKC17_RS11130 (GKC17_02525) - 482457..482927 (+) 471 WP_016396795.1 EAL domain-containing protein -
  GKC17_RS11210 - 482935..483202 (+) 268 Protein_457 EAL domain-containing protein -
  GKC17_RS02535 (GKC17_02540) - 483215..483553 (+) 339 WP_016396793.1 hypothetical protein -
  GKC17_RS02540 (GKC17_02545) - 483756..483962 (+) 207 WP_016396792.1 hypothetical protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50113.61 Da        Isoelectric Point: 6.6143

>NTDB_id=401475 GKC17_RS02510 WP_035165148.1 479858..481234(+) (radA) [Lactobacillus delbrueckii subsp. lactis strain MAG_rmk202_ldel]
MARARTQYKCRSCGYISASYLGRCPNCGAWNQFEKESQEVRQTSTKATPSRLMASVGAKEAVKMSTVKAEKEERVLTKSS
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSELAEKYKVLYVSGEESASQIKLRADRLGLGQSPMLLYPESNMEHI
RDQIDDVKPDFVVIDSIQTMNEPSLDSMVGSASQVREVTAELMKIAKLDQVTVFVIGHVTKEGSIAGPKILEHMVDTVLY
FEGDEHHAYRILHSVKNRFGAANEIGMFEMKTEGLAEVTNPSAVFLDERLPDSTGSAVVVSLEGTRPILGEIQALVTPTA
FGYAKRTTSGLDYNRVSLLLAVLEKRGNLMLQNQDAYLSATGGIRLNEPAIDLAIAMAIASSYYDKEIYATDCFVGEIGL
TGEVRRVNQIQARVKEAGKVGFKRIFIPKYNMSPALQDLGIEVIPVSSVRQALHYLFS

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=401475 GKC17_RS02510 WP_035165148.1 479858..481234(+) (radA) [Lactobacillus delbrueckii subsp. lactis strain MAG_rmk202_ldel]
ATGGCCAGAGCTAGAACCCAGTACAAGTGCCGGTCTTGCGGCTACATCTCCGCTTCCTACCTGGGCCGGTGCCCGAACTG
CGGGGCCTGGAACCAGTTTGAAAAAGAAAGCCAGGAGGTCCGCCAGACTTCTACTAAAGCTACTCCCAGCCGCTTGATGG
CCAGCGTGGGTGCTAAAGAAGCCGTTAAAATGTCCACCGTCAAGGCGGAAAAAGAAGAGCGGGTTTTAACCAAGTCCTCT
GAACTTAACCGGGTCTTAGGCGGCGGGATCGTACCAGGCTCTTTGGTCTTGATCGGCGGGGACCCCGGCATCGGCAAGTC
CACTTTGATGCTGCAGATCATGAGCGAGCTGGCCGAAAAATACAAGGTCCTCTACGTCTCCGGGGAAGAATCTGCCAGCC
AGATCAAGCTGCGGGCTGACCGCTTAGGCCTGGGCCAAAGTCCCATGCTTTTATACCCGGAAAGCAACATGGAACACATC
CGCGACCAGATCGATGACGTTAAGCCAGATTTTGTCGTGATCGACTCCATCCAGACCATGAACGAACCCAGCCTGGACTC
CATGGTGGGCTCAGCTTCCCAGGTCCGGGAAGTTACCGCGGAACTGATGAAGATCGCCAAGCTGGACCAAGTCACTGTCT
TTGTCATTGGCCACGTGACCAAGGAAGGGTCCATCGCCGGGCCGAAAATCCTGGAGCACATGGTTGACACCGTCCTTTAC
TTTGAAGGTGACGAGCACCATGCCTACCGGATCCTGCACTCAGTCAAGAACCGTTTTGGGGCGGCCAACGAAATCGGCAT
GTTTGAAATGAAGACCGAAGGCCTGGCAGAAGTGACCAATCCTTCCGCGGTCTTCTTAGATGAACGCCTGCCCGACTCAA
CTGGGTCAGCCGTGGTCGTTTCTTTAGAAGGTACCCGGCCGATCTTGGGCGAAATCCAGGCCCTGGTCACCCCAACCGCC
TTCGGCTATGCCAAGCGGACTACTTCGGGCCTGGACTACAACCGGGTCTCCCTGCTTTTAGCCGTTTTGGAAAAAAGGGG
GAACCTAATGCTGCAGAACCAGGACGCCTATTTATCAGCGACCGGCGGCATCCGCTTAAACGAACCGGCCATTGACCTGG
CCATCGCCATGGCCATTGCCTCCAGTTACTATGACAAGGAAATCTACGCGACTGACTGCTTTGTCGGGGAAATCGGCCTG
ACCGGGGAAGTCCGCCGGGTCAACCAGATCCAGGCCCGAGTCAAGGAAGCCGGCAAGGTTGGCTTCAAGCGGATTTTTAT
CCCGAAATACAATATGAGCCCGGCCTTGCAGGATTTGGGGATTGAGGTAATCCCGGTCTCCAGCGTCCGCCAGGCCCTCC
ACTACCTTTTCTCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

56.332

100

0.563
  radA Streptococcus mitis NCTC 12261

56.332

100

0.563
  radA Streptococcus pneumoniae Rx1

56.332

100

0.563
  radA Streptococcus pneumoniae D39

56.332

100

0.563
  radA Streptococcus pneumoniae R6

56.332

100

0.563
  radA Streptococcus pneumoniae TIGR4

56.332

100

0.563
  radA Bacillus subtilis subsp. subtilis str. 168

55.604

99.345

0.552

Multiple sequence alignment