Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   GKC27_RS11535 Genome accession   NZ_CP046130
Coordinates   2281158..2281928 (-) Length   256 a.a.
NCBI ID   WP_003217286.1    Uniprot ID   -
Organism   Bacillus pumilus strain DSM 27     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2276158..2286928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC27_RS11515 (GKC27_11400) - 2276251..2277072 (-) 822 WP_003217375.1 pyruvate, water dikinase regulatory protein -
  GKC27_RS11520 (GKC27_11405) - 2277098..2277736 (-) 639 WP_034620854.1 helix-turn-helix transcriptional regulator -
  GKC27_RS11525 (GKC27_11410) glyS 2277903..2279948 (-) 2046 WP_003217280.1 glycine--tRNA ligase subunit beta -
  GKC27_RS11530 (GKC27_11415) glyQ 2279941..2280828 (-) 888 WP_003217487.1 glycine--tRNA ligase subunit alpha -
  GKC27_RS11535 (GKC27_11420) recO 2281158..2281928 (-) 771 WP_003217286.1 DNA repair protein RecO Machinery gene
  GKC27_RS11540 (GKC27_11425) - 2281964..2282107 (-) 144 WP_003217288.1 YqzL family protein -
  GKC27_RS11545 (GKC27_11430) era 2282256..2283161 (-) 906 WP_003217394.1 GTPase Era -
  GKC27_RS11550 (GKC27_11435) - 2283154..2283552 (-) 399 WP_003217296.1 cytidine deaminase -
  GKC27_RS11555 (GKC27_11440) - 2283666..2284052 (-) 387 WP_003217363.1 diacylglycerol kinase family protein -
  GKC27_RS11560 (GKC27_11445) ybeY 2284030..2284509 (-) 480 WP_003217410.1 rRNA maturation RNase YbeY -
  GKC27_RS11565 (GKC27_11450) - 2284510..2286597 (-) 2088 WP_050782694.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29230.90 Da        Isoelectric Point: 8.4348

>NTDB_id=401449 GKC27_RS11535 WP_003217286.1 2281158..2281928(-) (recO) [Bacillus pumilus strain DSM 27]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADVILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYEEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=401449 GKC27_RS11535 WP_003217286.1 2281158..2281928(-) (recO) [Bacillus pumilus strain DSM 27]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTGATGGCAAGAGGCGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGAACC
ATTCGGGAAGATTTATTTTTGACCGCATATGCCGCATATATGACAGAGTTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTTTGCGTCATCTGAATGAAGGCACAGATGCTGATGTTATCTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGAATGAAGCCCGAACTGGATCAATGTGTCCACTGTGGTCAAAAGGAAGGG
CAGTTTCATTTCTCGATTAGAGACAACGGCTTTATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
TCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGAAGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTCATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

76.172

100

0.762

Multiple sequence alignment