Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GKC27_RS11260 Genome accession   NZ_CP046130
Coordinates   2231254..2232324 (-) Length   356 a.a.
NCBI ID   WP_003217401.1    Uniprot ID   -
Organism   Bacillus pumilus strain DSM 27     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2226254..2237324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC27_RS11210 (GKC27_11105) - 2226347..2226919 (-) 573 WP_003217306.1 signal peptidase I -
  GKC27_RS11215 (GKC27_11110) tapA 2226971..2227498 (-) 528 WP_003217325.1 amyloid fiber anchoring/assembly protein TapA -
  GKC27_RS11220 (GKC27_11115) - 2227798..2228076 (+) 279 WP_003217457.1 DUF3889 domain-containing protein -
  GKC27_RS11225 (GKC27_11120) - 2228110..2228304 (-) 195 WP_034620826.1 YqzE family protein -
  GKC27_RS11230 (GKC27_11125) comGG 2228364..2228747 (-) 384 WP_003217479.1 competence type IV pilus minor pilin ComGG -
  GKC27_RS11235 (GKC27_11130) comGF 2228744..2229274 (-) 531 WP_226803663.1 competence type IV pilus minor pilin ComGF -
  GKC27_RS11240 comGE 2229183..2229500 (-) 318 WP_003217390.1 competence type IV pilus minor pilin ComGE -
  GKC27_RS11245 (GKC27_11135) comGD 2229484..2229930 (-) 447 WP_003217424.1 competence type IV pilus minor pilin ComGD -
  GKC27_RS11250 (GKC27_11140) comGC 2229923..2230216 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  GKC27_RS11255 (GKC27_11145) comGB 2230233..2231273 (-) 1041 WP_003217311.1 competence type IV pilus assembly protein ComGB -
  GKC27_RS11260 (GKC27_11150) comGA 2231254..2232324 (-) 1071 WP_003217401.1 competence type IV pilus ATPase ComGA Machinery gene
  GKC27_RS11265 (GKC27_11155) - 2232502..2233374 (-) 873 WP_003217438.1 STAS domain-containing protein -
  GKC27_RS11275 (GKC27_11165) - 2233598..2233978 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  GKC27_RS11280 (GKC27_11170) - 2234191..2234433 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  GKC27_RS11285 (GKC27_11175) - 2234678..2235316 (-) 639 WP_003217463.1 MBL fold metallo-hydrolase -
  GKC27_RS11290 (GKC27_11180) - 2235474..2235647 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  GKC27_RS11295 (GKC27_11185) - 2235871..2236185 (-) 315 WP_003217504.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40450.95 Da        Isoelectric Point: 9.6540

>NTDB_id=401448 GKC27_RS11260 WP_003217401.1 2231254..2232324(-) (comGA) [Bacillus pumilus strain DSM 27]
MYGIEYLGQELLEEACRMRASDVHIVPKEKKASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELRECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=401448 GKC27_RS11260 WP_003217401.1 2231254..2232324(-) (comGA) [Bacillus pumilus strain DSM 27]
TTGTATGGGATTGAATACTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAGGAAAAGAAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCATTAGCT
GTGATGCTAAGAAGGGGTCAGGTGTTTATTCGGATGTCTACCCTACCAACTGTAAATGATGAGAGCTTAGTGATTAGAAT
ATTACCGCAGGATCATGTTCCAAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCACCAGGTTATTATCATTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTTTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACAGAAATATTATTACACTTGAAGATCCTGTGGAAACGAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGTATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTGGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTTCGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment