Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   GKC27_RS10060 Genome accession   NZ_CP046130
Coordinates   2019164..2019775 (+) Length   203 a.a.
NCBI ID   WP_003215988.1    Uniprot ID   -
Organism   Bacillus pumilus strain DSM 27     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2014164..2024775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC27_RS10020 (GKC27_09915) - 2014331..2015449 (+) 1119 WP_223251172.1 NADH-dependent flavin oxidoreductase -
  GKC27_RS10025 (GKC27_09920) - 2015501..2016007 (-) 507 WP_003216086.1 PTS glucose transporter subunit IIA -
  GKC27_RS10030 (GKC27_09925) - 2016175..2016594 (+) 420 WP_003215994.1 Hsp20/alpha crystallin family protein -
  GKC27_RS10035 (GKC27_09930) - 2016615..2017067 (-) 453 WP_003215482.1 YppG family protein -
  GKC27_RS10040 (GKC27_09935) - 2017174..2017554 (-) 381 WP_223251160.1 YppE family protein -
  GKC27_RS10045 (GKC27_09940) - 2017603..2017836 (-) 234 WP_231953199.1 DUF5446 family protein -
  GKC27_RS10050 (GKC27_09945) sspM 2018033..2018137 (+) 105 WP_012010346.1 acid-soluble spore protein SspM -
  GKC27_RS10055 (GKC27_09950) - 2018161..2019117 (-) 957 WP_003215456.1 DUF2515 domain-containing protein -
  GKC27_RS10060 (GKC27_09955) recU 2019164..2019775 (+) 612 WP_003215988.1 Holliday junction resolvase RecU Machinery gene
  GKC27_RS10065 (GKC27_09960) - 2019819..2022533 (+) 2715 WP_003216026.1 PBP1A family penicillin-binding protein -
  GKC27_RS10070 (GKC27_09965) - 2022769..2023278 (-) 510 WP_003215857.1 YpoC family protein -
  GKC27_RS10075 (GKC27_09970) nth 2023275..2023937 (-) 663 WP_003215968.1 endonuclease III -
  GKC27_RS10080 (GKC27_09975) - 2023955..2024659 (-) 705 WP_003215839.1 DnaD domain-containing protein -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23296.58 Da        Isoelectric Point: 9.4107

>NTDB_id=401443 GKC27_RS10060 WP_003215988.1 2019164..2019775(+) (recU) [Bacillus pumilus strain DSM 27]
MIRYPNGKSYQPIQPIGTKKRISGESSYSNRGMTLEADLNETNQYYLVNGIAVIHKKPTPVQIVNVDYPKRSAAVIKEAY
FKQSSTTDYNGVYKGRYIDFEAKETKSTTSFPLKNFHDHQIEHMKQVEKLGGICFVIISAFGSVYYLPAPDLFFFWERQK
LNGRKSISKDELMEAGHLMTLGYSPRIDYIKVVETLHFSDESK

Nucleotide


Download         Length: 612 bp        

>NTDB_id=401443 GKC27_RS10060 WP_003215988.1 2019164..2019775(+) (recU) [Bacillus pumilus strain DSM 27]
ATGATTCGGTATCCAAACGGAAAATCGTATCAGCCCATTCAACCAATTGGGACAAAAAAAAGAATCAGCGGCGAATCCTC
TTATAGTAACCGCGGGATGACGCTTGAGGCTGACTTAAATGAAACCAACCAGTACTATTTGGTCAATGGGATCGCAGTCA
TTCATAAAAAGCCTACCCCCGTTCAGATTGTGAATGTGGATTATCCAAAAAGAAGCGCTGCTGTGATCAAAGAAGCCTAT
TTTAAGCAATCATCCACAACAGACTATAATGGAGTGTACAAAGGGCGCTATATCGATTTTGAAGCAAAGGAGACCAAAAG
CACCACCTCGTTTCCGCTCAAAAATTTCCACGACCATCAAATTGAGCATATGAAACAGGTTGAAAAGCTAGGCGGCATTT
GTTTCGTTATTATATCCGCATTTGGCTCCGTTTATTATTTGCCCGCTCCAGATCTCTTTTTCTTCTGGGAGCGGCAGAAG
CTAAACGGCCGGAAATCCATTAGTAAAGATGAATTAATGGAAGCCGGCCACTTAATGACACTTGGATATTCGCCAAGAAT
TGATTATATTAAAGTAGTGGAGACACTTCATTTTTCGGATGAAAGTAAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

72.277

99.507

0.719

Multiple sequence alignment