Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GKC27_RS08710 Genome accession   NZ_CP046130
Coordinates   1753737..1754117 (+) Length   126 a.a.
NCBI ID   WP_223251255.1    Uniprot ID   -
Organism   Bacillus pumilus strain DSM 27     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1748737..1759117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC27_RS08700 (GKC27_08605) - 1749227..1752370 (+) 3144 WP_003211586.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  GKC27_RS08705 (GKC27_08610) - 1752567..1753475 (+) 909 WP_003212025.1 trypsin-like serine protease -
  GKC27_RS08710 (GKC27_08615) nucA/comI 1753737..1754117 (+) 381 WP_223251255.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GKC27_RS08715 (GKC27_08620) - 1754266..1755114 (+) 849 WP_003211492.1 STAS domain-containing protein -
  GKC27_RS08720 (GKC27_08625) - 1755148..1755690 (-) 543 WP_003211360.1 IseA DL-endopeptidase inhibitor family protein -
  GKC27_RS08725 (GKC27_08630) - 1755985..1756419 (+) 435 WP_003212431.1 hypothetical protein -
  GKC27_RS08730 (GKC27_08635) lexA 1756464..1757084 (-) 621 WP_003212311.1 transcriptional repressor LexA -
  GKC27_RS08735 (GKC27_08640) yneA 1757244..1757555 (+) 312 WP_003211955.1 cell division suppressor protein YneA -
  GKC27_RS08740 (GKC27_08645) - 1757573..1758220 (+) 648 WP_003211322.1 recombinase family protein -
  GKC27_RS08745 (GKC27_08650) - 1758290..1758520 (+) 231 WP_003211509.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13751.39 Da        Isoelectric Point: 7.3841

>NTDB_id=401442 GKC27_RS08710 WP_223251255.1 1753737..1754117(+) (nucA/comI) [Bacillus pumilus strain DSM 27]
MGTLLGGFGEEHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=401442 GKC27_RS08710 WP_223251255.1 1753737..1754117(+) (nucA/comI) [Bacillus pumilus strain DSM 27]
ATGGGCACGCTATTAGGTGGATTTGGGGAGGAACATGCGGCAAAAGGTGCCGATCGATATGATCATGTCATTCAATTTCC
AAAGGAACGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGCATTCCGACAAAACCTGGCTTTGACCGGGATGAGTGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGGCACCAAATCAGCGGATTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.143

94.444

0.54

Multiple sequence alignment