Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   GKC27_RS07815 Genome accession   NZ_CP046130
Coordinates   1568614..1569516 (+) Length   300 a.a.
NCBI ID   WP_003211145.1    Uniprot ID   -
Organism   Bacillus pumilus strain DSM 27     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1563614..1574516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC27_RS07795 (GKC27_07710) - 1564280..1566016 (+) 1737 WP_003212009.1 hypothetical protein -
  GKC27_RS07800 (GKC27_07715) - 1566013..1566288 (+) 276 WP_003211418.1 FlhB-like flagellar biosynthesis protein -
  GKC27_RS07805 (GKC27_07720) sucC 1566465..1567625 (+) 1161 WP_003211841.1 ADP-forming succinate--CoA ligase subunit beta -
  GKC27_RS07810 (GKC27_07725) sucD 1567648..1568550 (+) 903 WP_003212080.1 succinate--CoA ligase subunit alpha -
  GKC27_RS07815 (GKC27_07730) dprA 1568614..1569516 (+) 903 WP_003211145.1 DNA-processing protein DprA Machinery gene
  GKC27_RS07820 (GKC27_07735) topA 1569710..1571785 (+) 2076 WP_003212237.1 type I DNA topoisomerase -
  GKC27_RS07825 (GKC27_07740) trmFO 1571856..1573160 (+) 1305 WP_003212444.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  GKC27_RS07830 (GKC27_07745) xerC 1573223..1574140 (+) 918 WP_003211704.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34110.43 Da        Isoelectric Point: 8.5961

>NTDB_id=401438 GKC27_RS07815 WP_003211145.1 1568614..1569516(+) (dprA) [Bacillus pumilus strain DSM 27]
MYNVSERMIFHRLKGLISPSLLTKWWKVDPELYINEETHHFKQDRSLQTIDFTRLKQAEEKEFPIFQHIVQAYLKQNIHM
IPITSPLYPSTLKHIYDPPPVLFLKGNISYLNEEKSLGVVGTRVPSSYGEACVKKIVGDLVKEEWTIVSGLAKGIDGLAH
KECIRNKGKTIGIIAGGFQHLYPKEHVQMAEYMGEHHLLLSEHPPYVKPEKWHFPMRNRLISALTSGTIVIQCKEKSGSL
ITAYQALEQGKEVFAVAGSIFDPNSTGPARLIQQGAKLVHSTKDILEEFSFRSVQYTELS

Nucleotide


Download         Length: 903 bp        

>NTDB_id=401438 GKC27_RS07815 WP_003211145.1 1568614..1569516(+) (dprA) [Bacillus pumilus strain DSM 27]
ATGTACAACGTGTCCGAAAGAATGATTTTTCACCGCTTAAAAGGCCTCATCTCACCCTCTTTGTTAACAAAATGGTGGAA
AGTCGATCCTGAGCTATATATAAATGAAGAAACACATCATTTCAAACAGGATCGATCATTACAAACAATCGACTTTACCC
GCTTAAAACAAGCCGAAGAAAAAGAATTCCCCATTTTTCAACACATCGTTCAAGCCTATTTAAAGCAAAACATTCACATG
ATTCCCATCACATCACCCTTATATCCCAGCACACTAAAACATATTTATGATCCTCCCCCTGTGTTATTCCTAAAAGGAAA
CATATCGTATTTAAATGAAGAAAAAAGTTTAGGTGTAGTAGGCACACGAGTTCCATCGTCTTATGGAGAAGCATGTGTGA
AGAAAATTGTTGGTGATCTTGTAAAGGAAGAGTGGACGATTGTCAGTGGCTTAGCAAAAGGCATTGATGGACTTGCACAT
AAAGAGTGCATTAGGAATAAAGGGAAAACGATCGGTATTATAGCAGGCGGATTTCAGCACTTATACCCAAAAGAACATGT
GCAAATGGCTGAATACATGGGCGAGCATCATTTGCTTTTATCCGAGCATCCGCCTTACGTCAAACCAGAAAAGTGGCATT
TCCCCATGAGGAATCGTTTAATTAGTGCGCTCACAAGTGGAACCATCGTGATTCAGTGCAAAGAAAAGAGCGGTTCACTC
ATTACCGCGTATCAAGCACTTGAGCAAGGCAAAGAGGTATTTGCGGTTGCAGGATCAATCTTTGATCCTAATTCCACAGG
TCCAGCCAGACTTATACAGCAGGGAGCAAAGCTTGTTCATTCAACGAAGGATATTTTAGAGGAATTCTCCTTCCGCAGCG
TTCAATATACTGAACTCTCATAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

54.704

95.667

0.523
  dprA Lactococcus lactis subsp. cremoris KW2

38.889

96

0.373

Multiple sequence alignment