Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GKC27_RS01940 Genome accession   NZ_CP046130
Coordinates   355818..356246 (-) Length   142 a.a.
NCBI ID   WP_003214307.1    Uniprot ID   -
Organism   Bacillus pumilus strain DSM 27     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 350818..361246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC27_RS01910 (GKC27_01880) - 350971..351303 (+) 333 WP_003213855.1 YckD family protein -
  GKC27_RS01915 (GKC27_01885) - 351353..352117 (-) 765 WP_003213935.1 MBL fold metallo-hydrolase -
  GKC27_RS01920 (GKC27_01890) - 352233..352649 (+) 417 WP_003214483.1 winged helix-turn-helix transcriptional regulator -
  GKC27_RS01925 (GKC27_01895) - 352679..353485 (+) 807 WP_003214338.1 AAC(3) family N-acetyltransferase -
  GKC27_RS01930 (GKC27_01900) - 353487..353873 (-) 387 WP_003213827.1 competence protein ComJ -
  GKC27_RS01935 (GKC27_01905) - 353994..355691 (-) 1698 WP_003214067.1 methyl-accepting chemotaxis protein -
  GKC27_RS01940 (GKC27_01910) nucA/comI 355818..356246 (-) 429 WP_003214307.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GKC27_RS01945 (GKC27_01915) - 356614..358683 (+) 2070 WP_003214320.1 hydantoinase/oxoprolinase family protein -
  GKC27_RS01950 (GKC27_01920) - 358673..360658 (+) 1986 WP_003213841.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15914.84 Da        Isoelectric Point: 5.5536

>NTDB_id=401418 GKC27_RS01940 WP_003214307.1 355818..356246(-) (nucA/comI) [Bacillus pumilus strain DSM 27]
MKLLKIILLLLLIVVGVATGYIQLEQSKQETTNSSYDKTIHFPSERYPETAKHIEEAIDEGHSSVCTIDRKHSDEQREQS
LHGIPTKRGYDRDEWPMAMCKEGGTGASVKYISPSDNRGAGSWVGHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=401418 GKC27_RS01940 WP_003214307.1 355818..356246(-) (nucA/comI) [Bacillus pumilus strain DSM 27]
ATGAAACTTCTTAAAATCATTCTCTTGCTTTTACTGATCGTCGTTGGTGTAGCAACAGGCTATATTCAGCTGGAGCAAAG
TAAACAAGAGACGACGAATTCATCTTATGACAAGACCATTCACTTCCCATCAGAACGTTACCCTGAAACCGCGAAGCACA
TAGAAGAGGCAATTGATGAAGGGCATTCATCCGTATGCACGATTGACCGTAAACATAGCGATGAACAAAGAGAACAATCA
CTTCATGGCATCCCAACAAAGCGCGGGTATGATCGGGACGAATGGCCGATGGCGATGTGTAAGGAAGGAGGAACAGGAGC
TTCAGTCAAATATATTAGTCCTTCAGATAATAGAGGAGCCGGCTCATGGGTCGGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATACAATTTATCATCGATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.116

85.211

0.563

Multiple sequence alignment