Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   GKC27_RS00020 Genome accession   NZ_CP046130
Coordinates   3056..4168 (+) Length   370 a.a.
NCBI ID   WP_003215341.1    Uniprot ID   -
Organism   Bacillus pumilus strain DSM 27     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC27_RS00005 (GKC27_00005) dnaA 1..1341 (+) 1341 WP_003214781.1 chromosomal replication initiator protein DnaA -
  GKC27_RS00010 (GKC27_00010) dnaN 1538..2674 (+) 1137 WP_003214619.1 DNA polymerase III subunit beta -
  GKC27_RS00015 (GKC27_00015) yaaA 2824..3039 (+) 216 WP_003215257.1 S4 domain-containing protein YaaA -
  GKC27_RS00020 (GKC27_00020) recF 3056..4168 (+) 1113 WP_003215341.1 DNA replication/repair protein RecF Machinery gene
  GKC27_RS00025 (GKC27_00025) - 4186..4431 (+) 246 WP_003214686.1 extracellular matrix/biofilm biosynthesis regulator RemA family protein -
  GKC27_RS00030 (GKC27_00030) gyrB 4489..6405 (+) 1917 WP_034620352.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  GKC27_RS00035 (GKC27_00035) gyrA 6638..9139 (+) 2502 WP_003214767.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42581.69 Da        Isoelectric Point: 7.7384

>NTDB_id=401413 GKC27_RS00020 WP_003215341.1 3056..4168(+) (recF) [Bacillus pumilus strain DSM 27]
MYIQSLALTSYRNYEHTELQFDNKVNVMIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDQDYAKIEGRVIKKNGP
LPMQLVISKKGKKGKVNHIEQQKLSHYVGALNTIMFAPEDLSLVKGSPQIRRRFLDMEIGQVSAVYLHDLSLYQKILSQR
NHYLKQLQTRKQKDQAMLEVLTEQLIDAAAKVVKRRLTFTKQLEKWAQPLHFGISRELETLTLQYHTAIEVSEASDLSKI
KNSYEESFQKLRDREIDRGVTLWGPHRDDLLFFVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVEGIDHATLKEAEIFRVASGKVID

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=401413 GKC27_RS00020 WP_003215341.1 3056..4168(+) (recF) [Bacillus pumilus strain DSM 27]
ATGTACATTCAAAGTCTGGCGTTAACTTCATACCGAAACTATGAACACACCGAGCTTCAATTCGACAACAAGGTGAATGT
CATGATCGGTGAGAATGCCCAAGGTAAAACGAACTTGATGGAAGCGATCTATGTATTGTCGATGGCAAAGTCGCATCGTA
CGTCAAATGATAAAGAACTTATCCGATGGGACCAAGACTATGCTAAAATAGAAGGTAGAGTCATCAAAAAAAATGGTCCA
CTCCCAATGCAGCTCGTGATCTCAAAAAAAGGGAAAAAGGGCAAGGTCAATCACATTGAACAACAGAAGCTCAGTCATTA
TGTTGGTGCGTTAAACACCATCATGTTTGCACCAGAGGACTTAAGTCTTGTGAAGGGCAGCCCGCAAATCCGCAGAAGAT
TTCTCGATATGGAGATTGGACAAGTTTCTGCTGTCTACTTGCATGATTTATCGCTCTATCAAAAAATTCTCTCTCAGCGG
AATCATTACTTGAAACAATTGCAGACAAGAAAGCAAAAGGATCAAGCGATGCTGGAGGTTTTAACAGAGCAGTTGATTGA
TGCGGCAGCGAAAGTTGTCAAAAGACGACTGACTTTTACGAAACAGCTCGAGAAATGGGCGCAGCCGTTGCATTTTGGCA
TTTCTAGAGAGCTAGAAACACTCACGCTCCAATACCACACGGCGATAGAGGTATCAGAAGCGTCAGACTTGTCGAAAATA
AAGAATAGCTATGAAGAATCGTTTCAGAAACTAAGAGACAGAGAAATAGACCGAGGAGTGACGCTGTGGGGACCTCACAG
AGATGACCTTCTTTTCTTTGTGAATGGTCGGGATGTTCAGACATATGGCTCTCAAGGGCAGCAAAGAACAACAGCTCTTT
CACTAAAACTGGCAGAAATCGACTTGATACACGAAGAGATCGGAGAATATCCCATTCTTCTACTCGATGATGTTTTATCT
GAACTTGATGATTACAGACAGTCTCATTTGCTCCATACCATTCAGGGACGTGTACAGACCTTCGTCACCACAACAAGTGT
TGAGGGCATCGATCACGCCACCTTGAAAGAAGCGGAAATTTTCAGAGTAGCCAGTGGAAAAGTAATTGACTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

83.74

99.73

0.835

Multiple sequence alignment