Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GKC25_RS08280 Genome accession   NZ_CP046128
Coordinates   1679304..1679684 (+) Length   126 a.a.
NCBI ID   WP_045210079.1    Uniprot ID   -
Organism   Bacillus pumilus strain V17C11     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1674304..1684684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS08270 (GKC25_08205) - 1674792..1677935 (+) 3144 WP_034660764.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  GKC25_RS08275 (GKC25_08210) - 1678128..1679036 (+) 909 WP_095285213.1 trypsin-like serine protease -
  GKC25_RS08280 (GKC25_08215) nucA/comI 1679304..1679684 (+) 381 WP_045210079.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GKC25_RS08285 (GKC25_08220) - 1679832..1680680 (+) 849 WP_095285214.1 STAS domain-containing protein -
  GKC25_RS08290 (GKC25_08225) - 1680714..1681256 (-) 543 WP_034660768.1 IseA DL-endopeptidase inhibitor family protein -
  GKC25_RS08295 (GKC25_08230) - 1681551..1681988 (+) 438 WP_034660769.1 hypothetical protein -
  GKC25_RS08300 (GKC25_08235) lexA 1682036..1682656 (-) 621 WP_003212311.1 transcriptional repressor LexA -
  GKC25_RS08305 (GKC25_08240) yneA 1682816..1683127 (+) 312 WP_034660770.1 cell division suppressor protein YneA -
  GKC25_RS08310 (GKC25_08245) - 1683145..1683792 (+) 648 WP_003211322.1 recombinase family protein -
  GKC25_RS08315 (GKC25_08250) - 1683862..1684092 (+) 231 WP_034660771.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13766.45 Da        Isoelectric Point: 8.4984

>NTDB_id=401383 GKC25_RS08280 WP_045210079.1 1679304..1679684(+) (nucA/comI) [Bacillus pumilus strain V17C11]
MGTLLGGFGEKHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=401383 GKC25_RS08280 WP_045210079.1 1679304..1679684(+) (nucA/comI) [Bacillus pumilus strain V17C11]
ATGGGCACGTTGTTAGGTGGATTTGGGGAGAAACATGCGGCAAAAGGTGCGGATCGATATGATCATGTCATTCAATTTCC
AAAGGAACGGTACCCTGAAACAGGCAGCCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACTAAACCTGGCTTTGACCGGGATGAGTGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGTGCGAGTGTTCAATATGTCAGCCCATCTGATAATAGGGGAGCAGGCTCATG
GGTTGGACACCAGATCAGCGGTTATCCTGATGGGAAAAGAATTTTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment