Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HUK75_RS24430 Genome accession   NZ_CP054792
Coordinates   5191435..5192928 (+) Length   497 a.a.
NCBI ID   WP_023107818.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5431     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5186435..5197928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK75_RS24400 (HUK75_24345) - 5186647..5187456 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  HUK75_RS24405 (HUK75_24350) kdsD 5187870..5188850 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  HUK75_RS24410 (HUK75_24355) - 5188850..5189389 (+) 540 WP_003094350.1 KdsC family phosphatase -
  HUK75_RS24415 (HUK75_24360) lptC 5189398..5189970 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  HUK75_RS24420 (HUK75_24365) lptA 5189957..5190484 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  HUK75_RS24425 (HUK75_24370) lptB 5190484..5191209 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  HUK75_RS24430 (HUK75_24375) rpoN/rpoN1 5191435..5192928 (+) 1494 WP_023107818.1 RNA polymerase factor sigma-54 Machinery gene
  HUK75_RS24435 (HUK75_24380) hpf 5193006..5193314 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  HUK75_RS24440 (HUK75_24385) ptsN 5193328..5193792 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HUK75_RS24445 (HUK75_24390) rapZ 5193794..5194654 (+) 861 WP_003094361.1 RNase adapter RapZ -
  HUK75_RS24450 (HUK75_24395) - 5194684..5194956 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  HUK75_RS24455 (HUK75_24400) - 5195053..5195985 (-) 933 WP_003120918.1 ZIP family metal transporter -
  HUK75_RS24460 (HUK75_24405) - 5196004..5196615 (-) 612 WP_009315925.1 superoxide dismutase -
  HUK75_RS24465 (HUK75_24410) - 5196628..5197077 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56028.18 Da        Isoelectric Point: 4.4327

>NTDB_id=401357 HUK75_RS24430 WP_023107818.1 5191435..5192928(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain SE5431]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTAWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=401357 HUK75_RS24430 WP_023107818.1 5191435..5192928(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain SE5431]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAACTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGGCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCTGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGGATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477

Multiple sequence alignment