Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GKC25_RS01820 Genome accession   NZ_CP046128
Coordinates   339892..340320 (-) Length   142 a.a.
NCBI ID   WP_034664156.1    Uniprot ID   A0AAE3WLD3
Organism   Bacillus pumilus strain V17C11     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 334892..345320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS01790 (GKC25_01775) - 335048..335377 (+) 330 WP_034664467.1 YckD family protein -
  GKC25_RS01795 (GKC25_01780) - 335427..336191 (-) 765 WP_060595506.1 MBL fold metallo-hydrolase -
  GKC25_RS01800 (GKC25_01785) - 336307..336723 (+) 417 WP_034664465.1 Lrp/AsnC family transcriptional regulator -
  GKC25_RS01805 (GKC25_01790) - 336752..337558 (+) 807 WP_307499020.1 AAC(3) family N-acetyltransferase -
  GKC25_RS01810 (GKC25_01795) - 337560..337946 (-) 387 WP_106038505.1 competence protein ComJ -
  GKC25_RS01815 (GKC25_01800) - 338067..339763 (-) 1697 Protein_323 methyl-accepting chemotaxis protein -
  GKC25_RS01820 (GKC25_01805) nucA/comI 339892..340320 (-) 429 WP_034664156.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GKC25_RS01825 (GKC25_01810) - 340688..342757 (+) 2070 WP_034664154.1 hydantoinase/oxoprolinase family protein -
  GKC25_RS01830 (GKC25_01815) - 342747..344732 (+) 1986 WP_342689890.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15886.79 Da        Isoelectric Point: 5.5444

>NTDB_id=401357 GKC25_RS01820 WP_034664156.1 339892..340320(-) (nucA/comI) [Bacillus pumilus strain V17C11]
MKLLKIILLLLLIVVGVATGYIQLEQSKQETTNSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSVCTIDRKHSDEQRDQS
LHGIPTKRGYDRDEWPMAMCKEGGTGASVKYISPSDNRGAGSWVGHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=401357 GKC25_RS01820 WP_034664156.1 339892..340320(-) (nucA/comI) [Bacillus pumilus strain V17C11]
ATGAAACTTCTTAAAATCATTCTCTTGCTTTTACTAATCGTCGTTGGTGTAGCAACAGGCTATATTCAGCTGGAGCAAAG
TAAACAAGAGACGACGAATTCATCTTATGACAAAACCATTCACTTCCCATCAGATCGTTACCCAGAAACCGCGAAGCACA
TAGAAGAGGCAATTGATGAAGGCCATTCATCCGTATGTACGATTGACCGTAAACATAGCGATGAACAGAGAGACCAATCA
CTTCATGGCATCCCAACAAAGCGCGGGTATGATCGGGACGAATGGCCGATGGCGATGTGTAAGGAAGGAGGAACAGGAGC
TTCTGTCAAATATATCAGTCCTTCAGATAATAGGGGAGCAGGCTCATGGGTCGGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATACAATTTATCATCGATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.116

85.211

0.563

Multiple sequence alignment