Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   GKC25_RS00020 Genome accession   NZ_CP046128
Coordinates   3057..4169 (+) Length   370 a.a.
NCBI ID   WP_034660671.1    Uniprot ID   A0AAE3WL83
Organism   Bacillus pumilus strain V17C11     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS00005 (GKC25_00005) dnaA 1..1341 (+) 1341 WP_034660669.1 chromosomal replication initiator protein DnaA -
  GKC25_RS00010 (GKC25_00010) dnaN 1538..2674 (+) 1137 WP_034660670.1 DNA polymerase III subunit beta -
  GKC25_RS00015 (GKC25_00015) yaaA 2825..3040 (+) 216 WP_003215257.1 S4 domain-containing protein YaaA -
  GKC25_RS00020 (GKC25_00020) recF 3057..4169 (+) 1113 WP_034660671.1 DNA replication/repair protein RecF Machinery gene
  GKC25_RS00025 (GKC25_00025) - 4187..4432 (+) 246 WP_008360920.1 extracellular matrix/biofilm biosynthesis regulator RemA family protein -
  GKC25_RS00030 (GKC25_00030) gyrB 4490..6406 (+) 1917 WP_034660746.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  GKC25_RS00035 (GKC25_00035) gyrA 6639..9143 (+) 2505 WP_034660673.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42573.66 Da        Isoelectric Point: 7.3662

>NTDB_id=401352 GKC25_RS00020 WP_034660671.1 3057..4169(+) (recF) [Bacillus pumilus strain V17C11]
MYIQSLALTSYRNYEHTELQFDNKVNVMIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDQDYAKIEGRVIKKNGP
LPMQLVISKKGKKGKVNHIEQQKLSHYVGALNTIMFAPEDLSLVKGSPQIRRRFLDMEIGQVSAVYLHDLSLYQKILSQR
NHYLKQLQTRKQTDQAMLEVLTEQLIDAAVKVVKRRLTFTKQLEKWAQPLHFGISRELETLTLQYQTAIEVSEASDLSKI
KNSYEESFQKLRDREIDRGVTLWGPHRDDLLFFVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVEGIDHATLKEAEIFRVASGKVID

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=401352 GKC25_RS00020 WP_034660671.1 3057..4169(+) (recF) [Bacillus pumilus strain V17C11]
ATGTACATTCAAAGTCTGGCGTTAACTTCATACAGAAACTATGAACACACTGAGCTTCAATTCGACAACAAGGTGAATGT
CATGATCGGTGAGAATGCCCAAGGTAAAACGAACTTGATGGAAGCGATCTATGTATTGTCGATGGCAAAGTCGCATCGTA
CGTCAAATGATAAAGAACTTATCCGATGGGACCAAGACTATGCTAAAATAGAAGGTAGAGTCATCAAAAAAAATGGTCCA
CTCCCAATGCAGCTCGTGATCTCAAAAAAAGGGAAAAAGGGCAAGGTCAATCACATTGAACAACAGAAGCTCAGTCATTA
TGTTGGTGCGTTAAACACCATCATGTTTGCACCAGAGGACTTAAGTCTTGTGAAGGGCAGCCCGCAAATCCGCAGAAGAT
TCCTCGATATGGAGATTGGACAAGTTTCGGCTGTCTACTTGCATGATTTATCGCTCTATCAAAAAATCCTCTCTCAGCGG
AATCATTACTTGAAACAATTGCAGACAAGAAAGCAAACGGATCAAGCGATGCTGGAGGTTTTAACAGAGCAGTTGATTGA
TGCGGCAGTGAAAGTGGTCAAAAGACGACTGACTTTTACGAAACAGCTCGAGAAATGGGCGCAGCCGCTGCATTTTGGCA
TTTCTAGAGAGCTAGAAACACTCACGCTCCAATACCAGACGGCGATAGAGGTATCAGAAGCGTCAGACTTGTCGAAAATA
AAGAATAGCTATGAAGAATCGTTTCAGAAACTAAGAGACAGAGAAATAGACCGAGGAGTGACACTGTGGGGACCTCACAG
AGATGACCTTCTTTTCTTTGTGAATGGTCGGGATGTTCAGACATATGGCTCTCAAGGGCAGCAAAGAACAACAGCTCTTT
CACTAAAGCTGGCAGAAATCGACCTGATACATGAAGAAATCGGAGAATATCCCATTCTTCTACTCGATGATGTTTTATCT
GAACTTGATGATTACAGACAGTCTCATTTGCTCCATACCATTCAGGGACGTGTACAGACCTTCGTCACCACAACAAGTGT
TGAAGGCATCGATCACGCCACCTTGAAAGAAGCGGAAATTTTCAGAGTAGCCAGTGGAAAAGTAATTGACTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

83.469

99.73

0.832

Multiple sequence alignment