Detailed information NTG0401323

Bioinformatically predicted

Overview

• Name: spo0A
• Type: Regulator
• Locus tag: GKC22_RS10650
• Genome accession: NZ_CP046127
• Coordinates: 2130915..2131718 (-)
• Length: 267 a.a.
• NCBI ID: WP_012010520.1
• Uniprot ID: W6IDW7
• Organism: Bacillus pumilus strain P48C1
• Function: activation and repression of comK; repression of rok; repression of abrB (predicted from homology) Competence regulation

Genomic Context

Location: 2125915..2136718

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKC22_RS10635 (GKC22_10565)	-	2128135..2128371 (+)	237	WP_034661240.1	DUF2627 domain-containing protein	-
GKC22_RS10640 (GKC22_10570)	-	2128406..2129143 (-)	738	WP_034661241.1	glycerophosphodiester phosphodiesterase	-
GKC22_RS10645 (GKC22_10575)	-	2129370..2130470 (-)	1101	WP_066030680.1	Rap family tetratricopeptide repeat protein	-
GKC22_RS10650 (GKC22_10580)	spo0A	2130915..2131718 (-)	804	WP_012010520.1	sporulation transcription factor Spo0A	Regulator
GKC22_RS10655 (GKC22_10585)	spoIVB	2132004..2133281 (-)	1278	WP_034661244.1	SpoIVB peptidase	-
GKC22_RS10660 (GKC22_10590)	recN	2133480..2135213 (-)	1734	WP_106037972.1	DNA repair protein RecN	Machinery gene
GKC22_RS10665 (GKC22_10595)	argR	2135247..2135696 (-)	450	WP_034661246.1	transcriptional regulator ArgR	-
GKC22_RS10670 (GKC22_10600)	-	2135859..2136713 (-)	855	WP_034661248.1	TlyA family RNA methyltransferase	-

Sequence

Protein

• Length: 267 a.a.
• Molecular weight: 29918.44 Da
• Isoelectric Point: 6.3913

>NTDB_id=401323 GKC22_RS10650 WP_012010520.1 2130915..2131718(-) (spo0A) [Bacillus pumilus strain P48C1]
MEKIKVCVADDNRELVGLLTEYIEGQEDMEVLGVAYNGQECLTLFKDKEPDVLLLDIIMPHLDGLAVLERLRENNDMTKQ
PSVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNGTQVTHRSSSIQNSVLRNKPEPKRKNLDASITTI
IHEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTV
SMSKAKPTNSEFIAMVADRLRLEHRAS

Nucleotide

• Length: 804 bp

>NTDB_id=401323 GKC22_RS10650 WP_012010520.1 2130915..2131718(-) (spo0A) [Bacillus pumilus strain P48C1]
GTGGAGAAAATTAAAGTGTGTGTAGCTGATGACAATCGAGAGCTTGTTGGCCTTTTGACAGAGTATATTGAGGGACAGGA
AGATATGGAAGTACTTGGCGTGGCGTATAATGGCCAGGAATGTCTGACATTATTTAAAGATAAAGAGCCCGACGTTCTCC
TCTTAGATATTATCATGCCTCATTTAGACGGTCTTGCTGTACTGGAGCGCCTCCGTGAAAACAATGACATGACCAAACAG
CCAAGTGTGATTATGCTAACGGCGTTCGGTCAAGAAGATGTCACGAAAAAAGCAGTTGATTTGGGCGCATCCTATTTCAT
CTTAAAGCCATTTGATATGGAAAACCTAGTCGGTCATATTCGTCAAGTCAGTGGAAACGGCACACAAGTCACCCATCGAT
CTTCATCTATCCAAAACAGTGTCCTTCGGAACAAACCTGAACCGAAACGTAAAAACTTAGATGCGAGCATTACGACGATT
ATTCATGAGATTGGCGTACCCGCTCATATTAAAGGATATTTATACTTAAGAGAAGCGATTTCGATGGTGTACAATGACAT
TGAGCTTCTCGGAAGCATCACAAAAGTGTTGTACCCAGACATCGCGAAGAAATTTAACACGACTGCCAGCAGGGTAGAAC
GAGCGATTCGCCATGCGATTGAAGTTGCTTGGAGCAGAGGAAACATCGATTCCATTTCTTCACTCTTTGGTTATACAGTC
AGCATGTCTAAAGCGAAACCAACCAATTCTGAATTTATCGCGATGGTAGCTGATCGTCTGCGCTTAGAGCATAGAGCTAG
CTAG

Domains

Predicted by InterproScan.

• Sporulation initiation factor Spo0A C terminal (Spo0A_C) (157-260)

• Response regulator receiver domain (Response_reg) (6-120)

3D structure

• AlphaFold DB: W6IDW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
spo0A	Bacillus subtilis subsp. subtilis str. 168	88.433	100	0.888