Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | GKC22_RS08385 | Genome accession | NZ_CP046127 |
| Coordinates | 1705016..1705396 (+) | Length | 126 a.a. |
| NCBI ID | WP_045210079.1 | Uniprot ID | - |
| Organism | Bacillus pumilus strain P48C1 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 1675331..1709504 | 1705016..1705396 | within | 0 |
Gene organization within MGE regions
Location: 1675331..1709504
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GKC22_RS08230 (GKC22_08180) | - | 1675331..1676422 (+) | 1092 | WP_342689654.1 | Rap family tetratricopeptide repeat protein | - |
| GKC22_RS08235 | - | 1676425..1676559 (+) | 135 | WP_262417204.1 | hypothetical protein | - |
| GKC22_RS08240 (GKC22_08185) | - | 1676693..1678615 (+) | 1923 | WP_342689655.1 | T7SS effector LXG polymorphic toxin | - |
| GKC22_RS08245 (GKC22_08190) | - | 1678615..1679088 (+) | 474 | WP_034660749.1 | antitoxin YezG family protein | - |
| GKC22_RS08250 (GKC22_08195) | - | 1679360..1679746 (-) | 387 | Protein_1575 | macro domain-containing protein | - |
| GKC22_RS08255 (GKC22_08200) | - | 1679860..1680402 (+) | 543 | WP_034660750.1 | GNAT family N-acetyltransferase | - |
| GKC22_RS08260 (GKC22_08205) | - | 1680544..1681125 (+) | 582 | WP_034660751.1 | undecaprenyl-diphosphatase | - |
| GKC22_RS08265 (GKC22_08210) | - | 1681178..1681549 (-) | 372 | WP_034660752.1 | iron chaperone | - |
| GKC22_RS08270 (GKC22_08215) | - | 1682045..1682290 (-) | 246 | WP_034660753.1 | hypothetical protein | - |
| GKC22_RS08275 (GKC22_08220) | - | 1682724..1683377 (+) | 654 | WP_034660754.1 | CotZ-related putative spore coat protein | - |
| GKC22_RS08280 (GKC22_08225) | - | 1683452..1683796 (+) | 345 | WP_342689656.1 | S-Ena type endospore appendage | - |
| GKC22_RS08285 | - | 1683841..1684149 (+) | 309 | WP_342689657.1 | hypothetical protein | - |
| GKC22_RS08290 | - | 1684202..1684471 (+) | 270 | WP_342689658.1 | hypothetical protein | - |
| GKC22_RS08295 (GKC22_08235) | - | 1684729..1685841 (+) | 1113 | WP_060596408.1 | tetratricopeptide repeat protein | - |
| GKC22_RS08300 (GKC22_08240) | - | 1686303..1686539 (+) | 237 | WP_095285201.1 | hypothetical protein | - |
| GKC22_RS08305 (GKC22_08245) | - | 1686567..1687262 (-) | 696 | WP_342689659.1 | YoaK family protein | - |
| GKC22_RS08310 (GKC22_08250) | - | 1687297..1689162 (-) | 1866 | WP_342689683.1 | DNA ligase D | - |
| GKC22_RS08315 (GKC22_08255) | - | 1689277..1690125 (+) | 849 | WP_003211891.1 | Ku protein | - |
| GKC22_RS08320 (GKC22_08260) | - | 1690304..1690747 (-) | 444 | WP_106038674.1 | DUF2188 domain-containing protein | - |
| GKC22_RS08325 (GKC22_08265) | - | 1690908..1691381 (+) | 474 | WP_095285205.1 | VOC family protein | - |
| GKC22_RS08330 (GKC22_08270) | - | 1691430..1691843 (+) | 414 | WP_095285206.1 | Rrf2 family transcriptional regulator | - |
| GKC22_RS08335 (GKC22_08275) | - | 1691975..1692826 (+) | 852 | WP_095285207.1 | SDR family oxidoreductase | - |
| GKC22_RS08340 (GKC22_08280) | - | 1693017..1693733 (+) | 717 | WP_034660757.1 | (Fe-S)-binding protein | - |
| GKC22_RS08345 (GKC22_08285) | - | 1693755..1695176 (+) | 1422 | WP_034660758.1 | LutB/LldF family L-lactate oxidation iron-sulfur protein | - |
| GKC22_RS08350 (GKC22_08290) | - | 1695176..1695898 (+) | 723 | WP_106037735.1 | lactate utilization protein C | - |
| GKC22_RS08355 (GKC22_08295) | - | 1696042..1696950 (+) | 909 | WP_034660760.1 | DMT family transporter | - |
| GKC22_RS08360 (GKC22_08300) | - | 1696963..1697154 (-) | 192 | WP_034660761.1 | hypothetical protein | - |
| GKC22_RS08365 (GKC22_08305) | - | 1697265..1699481 (+) | 2217 | WP_034660762.1 | RNA ligase | - |
| GKC22_RS08370 (GKC22_08315) | - | 1699904..1700482 (+) | 579 | WP_034660763.1 | TetR/AcrR family transcriptional regulator | - |
| GKC22_RS08375 (GKC22_08320) | - | 1700504..1703647 (+) | 3144 | WP_106037257.1 | bifunctional cytochrome P450/NADPH--P450 reductase | - |
| GKC22_RS08380 (GKC22_08325) | - | 1703840..1704748 (+) | 909 | WP_095285213.1 | trypsin-like serine protease | - |
| GKC22_RS08385 (GKC22_08330) | nucA/comI | 1705016..1705396 (+) | 381 | WP_045210079.1 | NucA/NucB deoxyribonuclease domain-containing protein | Machinery gene |
| GKC22_RS08390 (GKC22_08335) | - | 1705544..1706392 (+) | 849 | WP_095285214.1 | STAS domain-containing protein | - |
| GKC22_RS08395 (GKC22_08340) | - | 1706426..1706968 (-) | 543 | WP_342689660.1 | IseA DL-endopeptidase inhibitor family protein | - |
| GKC22_RS08400 (GKC22_08345) | - | 1707263..1707700 (+) | 438 | WP_034660769.1 | hypothetical protein | - |
| GKC22_RS08405 (GKC22_08350) | lexA | 1707748..1708368 (-) | 621 | WP_003212311.1 | transcriptional repressor LexA | - |
| GKC22_RS08410 (GKC22_08355) | yneA | 1708528..1708839 (+) | 312 | WP_034660770.1 | cell division suppressor protein YneA | - |
| GKC22_RS08415 (GKC22_08360) | - | 1708857..1709504 (+) | 648 | WP_003211322.1 | recombinase family protein | - |
Sequence
Protein
Download Length: 126 a.a. Molecular weight: 13766.45 Da Isoelectric Point: 8.4984
>NTDB_id=401321 GKC22_RS08385 WP_045210079.1 1705016..1705396(+) (nucA/comI) [Bacillus pumilus strain P48C1]
MGTLLGGFGEKHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGYPDGKRILFIVK
MGTLLGGFGEKHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGYPDGKRILFIVK
Nucleotide
Download Length: 381 bp
>NTDB_id=401321 GKC22_RS08385 WP_045210079.1 1705016..1705396(+) (nucA/comI) [Bacillus pumilus strain P48C1]
ATGGGCACGTTGTTAGGTGGATTTGGGGAGAAACATGCGGCAAAAGGTGCGGATCGATATGATCATGTCATTCAATTTCC
AAAGGAACGGTACCCTGAAACAGGCAGCCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACTAAACCTGGCTTTGACCGGGATGAGTGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGTGCGAGTGTTCAATATGTCAGCCCATCTGATAATAGGGGAGCAGGCTCATG
GGTTGGACACCAGATCAGCGGTTATCCTGATGGGAAAAGAATTTTATTTATTGTGAAATAA
ATGGGCACGTTGTTAGGTGGATTTGGGGAGAAACATGCGGCAAAAGGTGCGGATCGATATGATCATGTCATTCAATTTCC
AAAGGAACGGTACCCTGAAACAGGCAGCCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACTAAACCTGGCTTTGACCGGGATGAGTGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGTGCGAGTGTTCAATATGTCAGCCCATCTGATAATAGGGGAGCAGGCTCATG
GGTTGGACACCAGATCAGCGGTTATCCTGATGGGAAAAGAATTTTATTTATTGTGAAATAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
57.983 |
94.444 |
0.548 |