Detailed information NTG0401302

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HUK75_RS02035
• Genome accession: NZ_CP054792
• Coordinates: 435996..437144 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain SE5431
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 430996..442144

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUK75_RS02010 (HUK75_02010)	-	432147..432443 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HUK75_RS02015 (HUK75_02015)	-	432440..433033 (-)	594	WP_003084544.1	YggT family protein	-
HUK75_RS02020 (HUK75_02020)	proC	433044..433865 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HUK75_RS02025 (HUK75_02025)	-	433877..434569 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HUK75_RS02030 (HUK75_02030)	pilT	434784..435818 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HUK75_RS02035 (HUK75_02035)	pilU	435996..437144 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
HUK75_RS02040 (HUK75_02040)	czcD	437152..438051 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HUK75_RS02045 (HUK75_02045)	-	438206..438610 (+)	405	WP_003084560.1	NINE protein	-
HUK75_RS02050 (HUK75_02050)	-	438853..440226 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
HUK75_RS02055 (HUK75_02055)	-	440223..441407 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=401302 HUK75_RS02035 WP_003110016.1 435996..437144(+) (pilU) [Pseudomonas aeruginosa strain SE5431]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=401302 HUK75_RS02035 WP_003110016.1 435996..437144(+) (pilU) [Pseudomonas aeruginosa strain SE5431]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382