Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GKC22_RS00595 Genome accession   NZ_CP046127
Coordinates   110819..112198 (+) Length   459 a.a.
NCBI ID   WP_034620767.1    Uniprot ID   A0AAE3WNQ6
Organism   Bacillus pumilus strain P48C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 105819..117198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC22_RS00575 (GKC22_00575) - 106161..106625 (+) 465 WP_003217184.1 CtsR family transcriptional regulator -
  GKC22_RS00580 (GKC22_00580) - 106640..107197 (+) 558 WP_034665655.1 UvrB/UvrC motif-containing protein -
  GKC22_RS00585 (GKC22_00585) - 107202..108293 (+) 1092 WP_342689373.1 protein arginine kinase -
  GKC22_RS00590 (GKC22_00590) clpC 108290..110725 (+) 2436 WP_034665649.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  GKC22_RS00595 (GKC22_00595) radA 110819..112198 (+) 1380 WP_034620767.1 DNA repair protein RadA Machinery gene
  GKC22_RS00600 (GKC22_00600) disA 112201..113280 (+) 1080 WP_012008686.1 DNA integrity scanning diadenylate cyclase DisA -
  GKC22_RS00605 (GKC22_00605) - 113434..114534 (+) 1101 WP_003217214.1 PIN/TRAM domain-containing protein -
  GKC22_RS00610 (GKC22_00610) ispD 114548..115237 (+) 690 WP_034665643.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GKC22_RS00615 (GKC22_00615) ispF 115241..115717 (+) 477 WP_095286191.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50024.54 Da        Isoelectric Point: 7.9305

>NTDB_id=401294 GKC22_RS00595 WP_034620767.1 110819..112198(+) (radA) [Bacillus pumilus strain P48C1]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSDEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIKSTSLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=401294 GKC22_RS00595 WP_034620767.1 110819..112198(+) (radA) [Bacillus pumilus strain P48C1]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAAACCGTCGAAGCGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCAGATGAACCCCGAATCAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTCATTGGCGGAGATCCTGGGATTGGGAAGTCCACATT
ATTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTATTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTCGGCATAAAAAGCACCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAAGAGATGAAACCCGCTTTTGTCGTGGTGGATTCGATTCAAACCGTTTATCAAAGCGATATTACGTCAGC
TCCTGGGTCTGTATCTCAAGTGAGAGAATGTACAGCACAGCTGATGAAAATTGCCAAGACAAATGGTATTCCAATATTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCTGGTCCACGCCTTTTAGAGCATATGGTGGACACGGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATCTTACGTGCGGTGAAAAACCGATTTGGCTCGACGAATGAACTAGGGATTTT
TGAAATGAGAGAGGAAGGACTCACGGAAGTATTAAACCCATCCGAAATTTTCTTAGAAGAACGTTCGGCAGGTGTATCTG
GATCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTCTTGTCGAGATACAAGCATTAATTTCACCGACAAGCTTT
GGAAATCCACGGAGAATGGCTACAGGCCTTGATCATAATCGTGTGTCATTGCTCATGGCGGTTTTAGAAAAACGTGTCGG
ACTGCTGCTGCAAAACCAAGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCGGCGATTGATTTGGCCA
TTGCGGTCAGTATTGCTTCAAGCTTTAAAGACGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCGAAACTAGGATTTAAGCGAATGTTTATTCC
TCAGGCGAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTCGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.118

99.782

0.869
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608

Multiple sequence alignment