Detailed information NTG0400768

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HUK64_RS22820
• Genome accession: NZ_CP054786
• Coordinates: 4916641..4917789 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain DL201330
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4911641..4922789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUK64_RS22795 (HUK64_22775)	-	4912792..4913088 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HUK64_RS22800 (HUK64_22780)	-	4913085..4913678 (-)	594	WP_003084544.1	YggT family protein	-
HUK64_RS22805 (HUK64_22785)	proC	4913689..4914510 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HUK64_RS22810 (HUK64_22790)	-	4914522..4915214 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
HUK64_RS22815 (HUK64_22795)	pilT	4915429..4916463 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HUK64_RS22820 (HUK64_22800)	pilU	4916641..4917789 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HUK64_RS22825 (HUK64_22805)	czcD	4917797..4918696 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HUK64_RS22830 (HUK64_22810)	-	4918850..4919254 (+)	405	WP_003084560.1	NINE protein	-
HUK64_RS22835 (HUK64_22815)	-	4919497..4920870 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HUK64_RS22840 (HUK64_22820)	-	4920867..4922051 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=400768 HUK64_RS22820 WP_003100959.1 4916641..4917789(+) (pilU) [Pseudomonas aeruginosa strain DL201330]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=400768 HUK64_RS22820 WP_003100959.1 4916641..4917789(+) (pilU) [Pseudomonas aeruginosa strain DL201330]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382