Detailed information NTG0400661

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GJ672_RS01115
• Genome accession: NZ_CP046046
• Coordinates: 222922..223929 (-)
• Length: 335 a.a.
• NCBI ID: WP_154295483.1
• Uniprot ID: A0A6B8JMX8
• Organism: Spiribacter sp. 2438
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 217922..228929

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GJ672_RS01085 (GJ672_01085)	dut	217985..218440 (-)	456	WP_154295478.1	dUTP diphosphatase	-
GJ672_RS01090 (GJ672_01090)	coaBC	218427..219620 (-)	1194	WP_154295479.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
GJ672_RS01095 (GJ672_01095)	radC	219704..220378 (+)	675	WP_154295480.1	DNA repair protein RadC	-
GJ672_RS01100 (GJ672_01100)	rpoH	220387..221277 (-)	891	WP_154295481.1	RNA polymerase sigma factor RpoH	-
GJ672_RS01105 (GJ672_01105)	ftsX	221346..222278 (-)	933	WP_229381905.1	permease-like cell division protein FtsX	-
GJ672_RS01110 (GJ672_01110)	ftsE	222275..222925 (-)	651	WP_154295482.1	cell division ATP-binding protein FtsE	-
GJ672_RS01115 (GJ672_01115)	pilA	222922..223929 (-)	1008	WP_154295483.1	signal recognition particle-docking protein FtsY	Machinery gene
GJ672_RS01120 (GJ672_01120)	-	224002..225348 (+)	1347	WP_154295484.1	pitrilysin family protein	-
GJ672_RS01125 (GJ672_01125)	-	225345..226664 (+)	1320	WP_154295485.1	pitrilysin family protein	-
GJ672_RS01130 (GJ672_01130)	rsmD	226669..227229 (+)	561	WP_154295486.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
GJ672_RS01135 (GJ672_01135)	coaD	227300..227812 (+)	513	WP_195759522.1	pantetheine-phosphate adenylyltransferase	-
GJ672_RS01140 (GJ672_01140)	-	227822..228151 (-)	330	WP_154295487.1	hypothetical protein	-
GJ672_RS01145 (GJ672_01145)	-	228151..228570 (-)	420	WP_195759523.1	DoxX family membrane protein	-

Sequence

Protein

• Length: 335 a.a.
• Molecular weight: 35677.51 Da
• Isoelectric Point: 4.9220

>NTDB_id=400661 GJ672_RS01115 WP_154295483.1 222922..223929(-) (pilA) [Spiribacter sp. 2438]
MLPFRRKHRAGDSEEKSTSPETAGSSGGLLERLRAGLRRTGSGLGQLFLGRKQIDEDLLDELETLLLMADVGVDATRSII
DDLTARLKRNELADTEALRLALREDMLRILEPVAHPLELPEQDGPVVVLTVGVNGAGKTTTIGKLAARLKDQGHGVMLAA
GDTFRAAAVEQLQAWGERTGVPVISQPTGADSASVVFDAHQSAVARGVDVMIADTAGRLHTQSNLMEELRKIHRVLGKQD
ERAPHEVLLVLDAGTGQNALAQAEQFQQAVSVTGLVLTKLDGTAKGGVIFAMAKRFGLPVYFIGVGEGVDDLRPFDAEAF
VDALLDQAGQSSDGP

Nucleotide

• Length: 1008 bp

>NTDB_id=400661 GJ672_RS01115 WP_154295483.1 222922..223929(-) (pilA) [Spiribacter sp. 2438]
ATGCTGCCATTTCGCCGCAAGCACCGCGCCGGTGATTCCGAAGAAAAATCCACGTCTCCGGAGACGGCAGGCTCCAGCGG
TGGCCTGCTGGAGCGGTTACGCGCCGGACTGCGGCGAACCGGCAGCGGCCTCGGGCAGCTGTTTCTCGGGCGCAAGCAGA
TCGACGAAGATCTGCTGGACGAGCTGGAAACGTTGCTCCTCATGGCCGATGTGGGCGTCGATGCCACCCGTTCGATCATT
GACGACCTGACCGCCCGACTCAAGCGCAACGAACTGGCGGATACCGAAGCACTGCGCCTGGCGCTTCGCGAAGACATGCT
CCGCATCCTCGAGCCGGTGGCCCACCCCCTGGAGCTGCCGGAACAGGATGGCCCGGTCGTCGTGCTGACCGTCGGCGTCA
ACGGCGCCGGCAAAACCACCACCATCGGCAAGCTCGCCGCCCGGCTGAAAGACCAGGGTCATGGCGTCATGCTGGCGGCC
GGCGATACCTTCCGGGCCGCCGCCGTCGAGCAGCTTCAGGCCTGGGGCGAACGCACCGGCGTGCCGGTGATCAGTCAGCC
CACCGGCGCCGACAGTGCTTCGGTGGTCTTTGATGCTCACCAATCTGCGGTCGCCCGAGGCGTGGATGTGATGATCGCGG
ACACCGCCGGCCGGCTGCATACCCAGAGCAACCTCATGGAAGAGCTCCGCAAGATCCATCGCGTTCTGGGCAAGCAGGAT
GAGCGCGCACCCCACGAGGTCCTGCTGGTGCTGGATGCGGGCACCGGCCAGAACGCCCTGGCCCAGGCCGAGCAGTTCCA
GCAGGCCGTCAGTGTCACCGGCCTGGTGCTGACCAAACTGGATGGCACCGCCAAGGGGGGCGTGATTTTCGCGATGGCCA
AGCGCTTCGGGCTGCCGGTCTACTTTATCGGTGTCGGTGAGGGAGTCGACGATCTGCGACCCTTTGACGCCGAGGCCTTT
GTGGACGCCTTACTCGACCAGGCCGGTCAGTCCAGCGACGGGCCATGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (41-105)

• SRP54-type protein, GTPase domain (SRP54) (126-325)

3D structure

• AlphaFold DB: A0A6B8JMX8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	54.305	90.149	0.49