Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GJD93_RS03900 Genome accession   NZ_CP046045
Coordinates   593575..594363 (+) Length   262 a.a.
NCBI ID   WP_371971077.1    Uniprot ID   -
Organism   Acinetobacter towneri strain 19110F47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 588575..599363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJD93_RS03870 (GJD93_03865) - 588684..589109 (+) 426 WP_004975083.1 hypothetical protein -
  GJD93_RS03875 (GJD93_03870) - 589375..590658 (+) 1284 WP_154320371.1 glycosyltransferase family 4 protein -
  GJD93_RS03880 (GJD93_03875) - 590684..591235 (+) 552 WP_154320372.1 phosphatase PAP2 family protein -
  GJD93_RS03885 (GJD93_03880) iscX 591337..591537 (+) 201 WP_004975089.1 Fe-S cluster assembly protein IscX -
  GJD93_RS03890 (GJD93_03885) ndk 591699..592130 (+) 432 WP_004975092.1 nucleoside-diphosphate kinase -
  GJD93_RS03895 (GJD93_03890) rlmN 592267..593499 (+) 1233 WP_004975094.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GJD93_RS03900 (GJD93_03895) pilF 593575..594363 (+) 789 WP_371971077.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GJD93_RS03905 (GJD93_03900) - 594354..595163 (+) 810 WP_004975098.1 helix-turn-helix domain-containing protein -
  GJD93_RS03910 (GJD93_03905) ispG 595186..596307 (+) 1122 WP_004975100.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GJD93_RS03915 (GJD93_03910) hisS 596311..597606 (+) 1296 WP_004975101.1 histidine--tRNA ligase -
  GJD93_RS03920 (GJD93_03915) - 597637..598338 (+) 702 WP_004975103.1 YfgM family protein -

Sequence

Protein


Download         Length: 262 a.a.        Molecular weight: 29451.33 Da        Isoelectric Point: 8.7703

>NTDB_id=400633 GJD93_RS03900 WP_371971077.1 593575..594363(+) (pilF) [Acinetobacter towneri strain 19110F47]
MAQITTVCISAIFAAACQTTAPTLGQKDPEKAVQVRTQLAAEYLKNGDLDSAKRALDQALESDSRDSTANMMMGVLLQQE
GSKLNLEKADAYFKRAIAADPKNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDIENAE
KSFKQALQANRDSYVSMLELSEILYLRQQFAPARQLYEQFVRAVGQKNQGARALWIGIRLARANGDQLGTQVLVNQLRAL
FPNSQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 789 bp        

>NTDB_id=400633 GJD93_RS03900 WP_371971077.1 593575..594363(+) (pilF) [Acinetobacter towneri strain 19110F47]
ATCGCACAAATTACAACTGTTTGTATTTCGGCAATTTTTGCTGCAGCTTGTCAAACCACAGCTCCAACCTTAGGGCAAAA
AGACCCTGAAAAAGCGGTGCAAGTACGTACGCAGTTGGCAGCAGAATACTTAAAAAATGGCGATTTAGACTCAGCCAAAC
GCGCACTTGATCAAGCTTTGGAAAGCGATTCACGTGATTCCACCGCTAATATGATGATGGGCGTGTTGCTGCAACAAGAA
GGCAGCAAGCTGAATTTAGAAAAAGCCGATGCGTATTTTAAGCGTGCCATTGCCGCTGACCCAAAAAATGCACAAGCGCG
TAACAACTATGGTACGTATTTATATCAAGTAGAACGCTATAATGATGCGATTGAGCAGCTCAGTATTGCAGGTGCAACAC
TGGGTTATGATCAACGCTTTAGAGCATTGGAAAATGTAGGTCGTATTTACTTGAAATTAGGCGATATAGAGAATGCCGAA
AAGTCATTCAAACAAGCCTTGCAAGCCAATCGCGATTCTTATGTTTCAATGTTAGAGTTGTCGGAAATTTTATATTTAAG
ACAACAATTTGCACCCGCGCGACAATTGTATGAACAATTTGTACGTGCTGTGGGGCAAAAAAATCAGGGTGCGCGTGCCT
TATGGATTGGGATTCGTTTAGCACGTGCCAATGGCGATCAATTGGGAACTCAGGTTTTAGTGAACCAGCTGCGTGCATTA
TTTCCCAATAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(93-125)

Tetratricopeptide repeat (TPR_16)

(39-76)

Tetratricopeptide repeat (TPR_16)

(147-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.778

96.183

0.748
  pilF Acinetobacter baylyi ADP1

72.047

96.947

0.698

Multiple sequence alignment