Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   GJ664_RS00755 Genome accession   NZ_CP046037
Coordinates   148801..149253 (-) Length   150 a.a.
NCBI ID   WP_076648209.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain CBA3614     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 143801..154253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJ664_RS00715 (GJ664_00715) - 143948..144313 (+) 366 WP_011374991.1 DUF805 domain-containing protein -
  GJ664_RS00725 (GJ664_00725) - 144856..145314 (-) 459 WP_035145008.1 hypothetical protein -
  GJ664_RS00730 (GJ664_00730) - 145369..146565 (-) 1197 WP_025016389.1 acetate/propionate family kinase -
  GJ664_RS00735 (GJ664_00735) - 146587..147597 (-) 1011 WP_025016388.1 class I SAM-dependent methyltransferase -
  GJ664_RS00740 (GJ664_00740) - 147721..148059 (-) 339 WP_056947123.1 hypothetical protein -
  GJ664_RS00745 (GJ664_00745) comGF 148022..148534 (-) 513 WP_025016386.1 competence type IV pilus minor pilin ComGF Machinery gene
  GJ664_RS00750 (GJ664_00750) comGE 148509..148814 (-) 306 WP_025016385.1 hypothetical protein Machinery gene
  GJ664_RS00755 (GJ664_00755) comGD 148801..149253 (-) 453 WP_076648209.1 competence type IV pilus minor pilin ComGD Machinery gene
  GJ664_RS00760 (GJ664_00760) comGC 149225..149524 (-) 300 WP_076645268.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GJ664_RS00765 (GJ664_00765) comGB 149521..150528 (-) 1008 WP_076648212.1 type II secretion system F family protein Machinery gene
  GJ664_RS00770 (GJ664_00770) comGA 150521..151411 (-) 891 WP_076648214.1 competence type IV pilus ATPase ComGA Machinery gene
  GJ664_RS00775 (GJ664_00775) - 151526..152257 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  GJ664_RS00780 (GJ664_00780) - 152348..152848 (-) 501 WP_016265379.1 VanZ family protein -
  GJ664_RS00785 (GJ664_00785) - 152945..153319 (+) 375 WP_011375004.1 hypothetical protein -
  GJ664_RS00795 (GJ664_00795) - 153506..154120 (-) 615 WP_016265380.1 hypothetical protein -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 17482.40 Da        Isoelectric Point: 10.2280

>NTDB_id=400404 GJ664_RS00755 WP_076648209.1 148801..149253(-) (comGD) [Latilactobacillus sakei strain CBA3614]
MKAARLSKNEGFTLIEMCVVLAIVSLLSWLPIYQIKQYRAQQAEQLFLHQFETSWDAARQYVAIEPRAVRVMWDAPTHTI
TFKGAGEAFRNHQLVLPETLTVSNPAEWHLINITHNKGIKPRTLKLKSTLNHREYQYKVQMMWGVLHVQK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=400404 GJ664_RS00755 WP_076648209.1 148801..149253(-) (comGD) [Latilactobacillus sakei strain CBA3614]
TTGAAGGCGGCACGGTTAAGCAAAAATGAAGGTTTTACGTTGATTGAAATGTGCGTGGTCTTAGCGATTGTGAGCTTATT
AAGCTGGTTGCCGATTTATCAGATTAAGCAATATCGTGCGCAACAGGCTGAGCAGTTGTTTCTACATCAATTTGAAACGA
GTTGGGATGCAGCTCGTCAATATGTGGCAATTGAACCGCGTGCCGTCCGTGTGATGTGGGATGCACCAACACATACGATC
ACATTTAAAGGTGCTGGTGAGGCGTTTAGAAATCACCAACTTGTATTACCAGAAACTTTAACAGTGAGTAATCCAGCCGA
ATGGCATTTAATTAATATTACTCATAATAAGGGTATCAAACCGCGCACCTTAAAGTTAAAATCAACGCTTAATCATCGTG
AATATCAGTATAAAGTCCAGATGATGTGGGGGGTATTACATGTTCAGAAGTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Latilactobacillus sakei subsp. sakei 23K

98

100

0.98

Multiple sequence alignment