Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   F7R28_RS04490 Genome accession   NZ_CP046023
Coordinates   957456..958592 (-) Length   378 a.a.
NCBI ID   WP_122954398.1    Uniprot ID   A0A5Q3H7Z4
Organism   Polaromonas sp. Pch-P     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 952456..963592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7R28_RS04465 (F7R28_04510) rsmI 953520..954368 (-) 849 WP_194965981.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  F7R28_RS04470 (F7R28_04515) - 954419..954886 (+) 468 WP_122954402.1 YraN family protein -
  F7R28_RS04475 (F7R28_04520) - 955122..955721 (+) 600 WP_122954401.1 phosphoheptose isomerase -
  F7R28_RS04480 (F7R28_04525) - 955718..956377 (+) 660 WP_122954400.1 BON domain-containing protein -
  F7R28_RS04485 (F7R28_04530) - 956453..957367 (-) 915 WP_122954399.1 NAD(P)-dependent oxidoreductase -
  F7R28_RS04490 (F7R28_04535) pilU 957456..958592 (-) 1137 WP_122954398.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  F7R28_RS04495 (F7R28_04540) pilT 958646..959689 (-) 1044 WP_122954397.1 type IV pilus twitching motility protein PilT Machinery gene
  F7R28_RS04500 (F7R28_04545) - 959739..960440 (+) 702 WP_122954396.1 YggS family pyridoxal phosphate-dependent enzyme -
  F7R28_RS04505 (F7R28_04550) - 960560..961108 (-) 549 WP_122954395.1 DUF3617 domain-containing protein -
  F7R28_RS04510 (F7R28_04555) - 961197..961676 (-) 480 WP_092130297.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41888.14 Da        Isoelectric Point: 6.9132

>NTDB_id=400259 F7R28_RS04490 WP_122954398.1 957456..958592(-) (pilU) [Polaromonas sp. Pch-P]
MERDQASKFINDLLKLMVSRNGSDLFITGDFPPAMKVDGKVTKVSPQPLNAGHTLALARSIMNDKQAAEFERTKECNFAI
SPPGVGRFRVNAFIQQGKVGMVMRTIPATLPTIDGLGVPQVLKEVVMSKRGLTILVGSTGSGKSTTLAAMVDWRNEQSYG
HIITIEDPVEFVHAHKNCVITQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRVINFFPEERRTQLLMDLSLNLKAMISQRLVPKQDGKGRFAAVEVMLNSPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQALYDAFESHFITYEDALRNADSVNDLRLQIKLNSQRAKSTDLSAGTENFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=400259 F7R28_RS04490 WP_122954398.1 957456..958592(-) (pilU) [Polaromonas sp. Pch-P]
ATGGAACGCGACCAGGCCAGTAAATTCATCAACGACTTGCTCAAACTGATGGTCAGCCGTAACGGCAGTGACTTGTTCAT
CACGGGCGACTTCCCGCCGGCCATGAAGGTCGACGGCAAGGTGACCAAGGTGTCGCCGCAGCCGCTGAACGCCGGGCACA
CGCTGGCGCTGGCCCGTTCCATCATGAACGACAAACAGGCGGCCGAGTTTGAGCGCACCAAGGAATGCAATTTCGCCATC
TCGCCGCCGGGCGTGGGGCGTTTCCGGGTCAATGCCTTCATCCAGCAGGGCAAGGTCGGCATGGTGATGCGGACGATTCC
CGCGACCCTGCCCACCATCGATGGCCTGGGAGTGCCCCAGGTGCTCAAGGAAGTCGTCATGAGCAAGCGCGGCCTGACCA
TCCTGGTCGGCTCCACCGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTCGACTGGCGCAACGAGCAGTCTTACGGC
CACATCATCACGATTGAAGACCCGGTGGAGTTTGTGCACGCGCACAAGAACTGCGTGATCACCCAGCGTGAAGTGGGCCT
GGACACCGACAGCTGGGAAGCCGCCCTGAAAAACACGCTGCGCCAGGCGCCCGACGTGATTTTGATGGGTGAGATCCGCG
ACCGCGAAACCATGGAACACGCCATCGCATTCGCCGAAACCGGCCACCTTTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCGTGATCAACTTCTTCCCCGAAGAGCGCCGCACGCAGCTGCTGATGGACTTGTCGCTCAATCT
CAAGGCCATGATCTCGCAGCGCCTGGTCCCCAAGCAGGACGGCAAGGGCCGTTTTGCCGCGGTCGAGGTGATGTTGAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAAGTCTCCGAGATCAAGGAGATCATGAAAAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTACGACGCGTTCGAGAGCCACTTCATCACGTATGAAGACGCACTGCGCAATGCCGA
CTCGGTGAACGATTTGCGCCTGCAGATCAAGCTCAACTCCCAACGCGCCAAATCGACCGACCTGTCGGCAGGCACCGAGA
ACTTCGCCATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q3H7Z4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.751

92.328

0.579
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baumannii strain A118

45.141

84.392

0.381
  pilT Acinetobacter nosocomialis M2

45.141

84.392

0.381
  pilT Acinetobacter baumannii D1279779

45.141

84.392

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.849

83.862

0.368
  pilT Vibrio cholerae strain A1552

43.849

83.862

0.368
  pilT Legionella pneumophila strain Lp02

41.742

88.095

0.368
  pilT Legionella pneumophila strain ERS1305867

41.742

88.095

0.368
  pilT Acinetobacter baylyi ADP1

43.26

84.392

0.365

Multiple sequence alignment