Detailed information NTG0400109

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GJG85_RS14860
• Genome accession: NZ_CP045994
• Coordinates: 3190558..3191718 (+)
• Length: 386 a.a.
• NCBI ID: WP_050014853.1
• Uniprot ID: -
• Organism: Burkholderia sp. MS389
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3185558..3196718

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GJG85_RS14830 (GJG85_14865)	-	3186195..3186800 (+)	606	WP_011546248.1	50S ribosomal protein L25/general stress protein Ctc	-
GJG85_RS14835 (GJG85_14870)	pth	3186921..3187520 (+)	600	WP_050014859.1	aminoacyl-tRNA hydrolase	-
GJG85_RS14840 (GJG85_14875)	hisC	3187638..3188705 (+)	1068	WP_050014857.1	histidinol-phosphate transaminase	-
GJG85_RS14845 (GJG85_14880)	-	3188794..3189060 (-)	267	WP_006477774.1	YfhL family 4Fe-4S dicluster ferredoxin	-
GJG85_RS14850 (GJG85_14885)	coaD	3189120..3189617 (-)	498	WP_044847476.1	pantetheine-phosphate adenylyltransferase	-
GJG85_RS14855 (GJG85_14890)	rsmD	3189729..3190343 (-)	615	WP_050014855.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
GJG85_RS14860 (GJG85_14895)	pilA	3190558..3191718 (+)	1161	WP_050014853.1	signal recognition particle-docking protein FtsY	Machinery gene
GJG85_RS14865 (GJG85_14900)	maiA	3191815..3192459 (-)	645	WP_050014852.1	maleylacetoacetate isomerase	-
GJG85_RS14870 (GJG85_14905)	ybiB	3192641..3193615 (+)	975	WP_050014850.1	DNA-binding protein YbiB	-
GJG85_RS14875 (GJG85_14910)	leuA	3193993..3195639 (+)	1647	WP_089488833.1	2-isopropylmalate synthase	-
GJG85_RS14880 (GJG85_14915)	-	3195772..3196155 (-)	384	WP_011886076.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 40986.74 Da
• Isoelectric Point: 4.6402

>NTDB_id=400109 GJG85_RS14860 WP_050014853.1 3190558..3191718(+) (pilA) [Burkholderia sp. MS389]
MFSFFKRFKKTQEPDPAESQSADAQQADEASDADAPAPEAPPAADVPQASAQAAAPAVVVTVTPTNDGRDEVVETVEIVP
PPLQDASAKKSWLARLKTGLAKTGSSITGVFVNTKIDEDLYEELETALLMSDAGVDATEYLLDALREKVRAGRLTDPQQV
KDALHDLLVELLTPLEKSLMLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERN
NVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVIAKAHDGAPHEVLLVIDANTGQNAL
TQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAVEFADALLG

Nucleotide

• Length: 1161 bp

>NTDB_id=400109 GJG85_RS14860 WP_050014853.1 3190558..3191718(+) (pilA) [Burkholderia sp. MS389]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAACGCAGGAGCCCGATCCGGCGGAATCGCAATCGGCCGACGCGCAGCAAGC
GGACGAAGCGTCCGACGCCGACGCGCCCGCGCCGGAGGCGCCGCCCGCGGCCGACGTGCCGCAGGCGAGCGCGCAAGCGG
CCGCGCCGGCCGTCGTGGTGACGGTCACGCCGACCAACGACGGCCGCGACGAAGTGGTCGAGACGGTCGAGATCGTCCCG
CCGCCGCTGCAGGACGCGTCCGCGAAGAAGTCGTGGCTCGCGCGCCTGAAAACGGGGCTCGCGAAGACGGGCTCGAGCAT
CACCGGCGTCTTCGTCAACACGAAGATCGACGAGGACCTGTACGAGGAGCTCGAAACCGCGCTGCTGATGTCCGACGCGG
GCGTCGACGCGACCGAGTACCTGCTCGACGCGCTGCGCGAGAAGGTGCGCGCGGGCCGGCTGACCGATCCGCAGCAGGTG
AAGGATGCGCTGCACGACCTGCTCGTCGAGCTGCTGACGCCGCTCGAGAAATCGCTGATGCTCGGCCGCGCGCAGCCGCT
CGTAATGATGATCGCCGGCGTGAACGGCGCGGGCAAGACGACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTTG
ACCAGTCGGTGCTGCTGGCCGCCGGCGACACGTTCCGCGCGGCCGCGCGCGAGCAGCTGGCGGTCTGGGGCGAGCGCAAC
AACGTGACGGTCGTGCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAA
GATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAACTGCACCTGATGGAAGAGCTGAAGAAGGTGAAGC
GCGTGATCGCGAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTGGTGATCGACGCGAACACCGGCCAGAACGCGCTC
ACGCAGGTGAAGGCATTCGACGACGCGCTGGGCCTGACCGGCCTGATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGG
GATTCTCGCCGCGATCGCGCGGCAACGGCCGGTGCCCGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACCTGCAGC
CGTTCAGCGCGGTGGAATTCGCGGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-386)

• SRP54-type protein, helical bundle domain (SRP54_N) (104-166)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	54.087	100	0.583