Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GJG85_RS14765 Genome accession   NZ_CP045994
Coordinates   3173760..3175265 (+) Length   501 a.a.
NCBI ID   WP_050014879.1    Uniprot ID   A0AAP9ZYF2
Organism   Burkholderia sp. MS389     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3168760..3180265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJG85_RS14740 (GJG85_14775) kdsD 3169991..3170974 (+) 984 WP_089488829.1 arabinose 5-phosphate isomerase KdsD -
  GJG85_RS14745 (GJG85_14780) - 3170974..3171510 (+) 537 WP_050014885.1 HAD family hydrolase -
  GJG85_RS14750 (GJG85_14785) lptC 3171514..3172128 (+) 615 WP_012329238.1 LPS export ABC transporter periplasmic protein LptC -
  GJG85_RS14755 (GJG85_14790) lptA 3172149..3172814 (+) 666 WP_050014883.1 lipopolysaccharide transport periplasmic protein LptA -
  GJG85_RS14760 (GJG85_14795) lptB 3172811..3173587 (+) 777 WP_050014881.1 LPS export ABC transporter ATP-binding protein -
  GJG85_RS14765 (GJG85_14800) rpoN/rpoN1 3173760..3175265 (+) 1506 WP_050014879.1 RNA polymerase factor sigma-54 Machinery gene
  GJG85_RS14770 (GJG85_14805) raiA 3175425..3175784 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  GJG85_RS14775 (GJG85_14810) - 3175979..3176491 (+) 513 WP_027806888.1 PTS sugar transporter subunit IIA -
  GJG85_RS14780 (GJG85_14815) hprK 3176621..3177589 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  GJG85_RS14785 (GJG85_14820) rapZ 3177664..3178572 (+) 909 WP_050014875.1 RNase adapter RapZ -
  GJG85_RS14790 (GJG85_14825) - 3178600..3179235 (+) 636 WP_050014873.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54749.90 Da        Isoelectric Point: 4.8740

>NTDB_id=400108 GJG85_RS14765 WP_050014879.1 3173760..3175265(+) (rpoN/rpoN1) [Burkholderia sp. MS389]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNGSSTSSDRAERDEPAGADEYDGYGPGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLAAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=400108 GJG85_RS14765 WP_050014879.1 3173760..3175265(+) (rpoN/rpoN1) [Burkholderia sp. MS389]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAGAACGACGACGAATGGA
TCGCCAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCGGCCGCG
ACGAACGGCAGCAGCACGTCCAGCGACCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGTTACGGGCC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTCGCCGCGCAATTGCGCGTCACGCAAGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAACTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCCACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACCCGGTTGCGCAAGCACCTGAAGGCGAG
CGACGATGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAACAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCGTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment