Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GJG85_RS10715 Genome accession   NZ_CP045994
Coordinates   2314476..2315852 (-) Length   458 a.a.
NCBI ID   WP_050012918.1    Uniprot ID   A0A228HY51
Organism   Burkholderia sp. MS389     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2309476..2320852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJG85_RS10695 (GJG85_10715) cls 2309791..2311230 (+) 1440 WP_089488570.1 cardiolipin synthase -
  GJG85_RS10700 (GJG85_10720) - 2311296..2311775 (-) 480 WP_089488571.1 glutathione peroxidase -
  GJG85_RS10705 (GJG85_10725) - 2311912..2313843 (+) 1932 WP_050012916.1 ATP-binding cassette domain-containing protein -
  GJG85_RS10710 (GJG85_10730) - 2314061..2314327 (-) 267 WP_050012917.1 DUF2866 domain-containing protein -
  GJG85_RS10715 (GJG85_10735) radA 2314476..2315852 (-) 1377 WP_050012918.1 DNA repair protein RadA Machinery gene
  GJG85_RS10720 (GJG85_10740) alr 2315864..2316934 (-) 1071 WP_050012919.1 alanine racemase -
  GJG85_RS10725 (GJG85_10745) lplT 2317153..2318460 (+) 1308 WP_050012920.1 lysophospholipid transporter LplT -
  GJG85_RS10730 (GJG85_10750) thiD 2318519..2319328 (+) 810 WP_050012921.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  GJG85_RS10735 (GJG85_10755) - 2319347..2320312 (-) 966 WP_204383300.1 DUF1853 family protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48788.19 Da        Isoelectric Point: 6.9557

>NTDB_id=400104 GJG85_RS10715 WP_050012918.1 2314476..2315852(-) (radA) [Burkholderia sp. MS389]
MAKQKTVYVCSECGGQTPKWQGQCPSCQAWNTLVESVAESPAAHRFQSLAKRAPVQRLADIEAADVPRFSTGIGEFDRVL
GGGLVPGGVVLIGGDPGIGKSTLLLQSLADIANERRALYISGEESAAQIALRAQRLALLDGGAPAAELKLLAEIQLEKIQ
AAIDAERPDVAVIDSIQTVYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQVVPGSCVLVTQEGTRPLLVEIQALVDTAHV
PNPRRLAVGLEQNRLAMLLAVLHRHAGIAAFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKALPKGLIVFGEVGLA
GEIRPSPRGQERLREAAKLGFTSALIPKANAPKQPIEGLNVMAVERLEQAIDRVRDLE

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=400104 GJG85_RS10715 WP_050012918.1 2314476..2315852(-) (radA) [Burkholderia sp. MS389]
GTGGCCAAACAGAAAACCGTATACGTCTGCAGCGAGTGCGGCGGACAGACCCCGAAGTGGCAGGGCCAGTGCCCGTCGTG
CCAGGCGTGGAACACGCTCGTCGAATCGGTCGCCGAGTCGCCGGCCGCCCATCGGTTCCAGTCGCTCGCGAAGCGGGCGC
CGGTCCAGCGTCTCGCCGACATCGAGGCTGCCGACGTCCCGCGCTTCTCGACCGGCATCGGCGAATTCGACCGCGTGCTG
GGCGGCGGGCTGGTGCCCGGCGGCGTCGTGCTGATCGGTGGCGATCCGGGCATCGGCAAGTCGACGCTGCTGCTGCAGTC
GCTCGCGGATATCGCGAACGAGCGGCGCGCGCTCTATATCAGTGGCGAGGAATCGGCCGCGCAGATCGCGTTGCGCGCGC
AACGGCTCGCGCTGCTCGACGGCGGCGCGCCGGCGGCCGAACTGAAGCTGCTCGCCGAGATCCAGCTCGAGAAGATCCAG
GCCGCGATCGACGCGGAGCGGCCGGACGTGGCCGTCATCGACTCGATCCAGACCGTCTATTCCGAGGCGCTGACGTCGGC
GCCGGGCTCGGTCGCGCAGGTGCGCGAGTGCGCGGCGCAGCTCACGCGCATCGCGAAACAGTCGGGCACCGCGATCATCA
TGGTCGGGCACGTGACGAAGGAGGGTAACCTCGCCGGCCCGCGCGTGCTCGAGCACATCGTCGACACGGTGCTGTACTTC
GAGGGCGACACGCATTCGTCGTTCCGGCTCGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAACTCGGCGTGTT
CGCGATGACCGAGCGCGGGCTGCGCGGCGTGGCCAACCCGTCCGCGCTGTTCCTGTCGCAGCACGAGCAGGTCGTGCCCG
GTTCGTGCGTGCTCGTCACGCAGGAGGGCACGCGCCCGCTGCTCGTCGAAATCCAGGCGCTCGTCGATACGGCGCACGTG
CCGAACCCGCGCCGGCTCGCGGTCGGCCTCGAGCAGAACCGGCTCGCGATGCTGCTTGCGGTGCTGCACCGCCACGCGGG
CATCGCGGCCTTCGATCAGGATGTGTTCCTGAACGCGGTGGGCGGCGTGAAGATCACCGAGCCGGCCGCCGACCTCGCGG
TGCTGCTCGCCATTCACTCGTCGATGCGCAACAAGGCGCTGCCGAAAGGGCTGATCGTGTTCGGCGAGGTGGGGCTGGCC
GGCGAGATCCGGCCGTCCCCGCGCGGGCAGGAACGGCTGCGCGAGGCGGCGAAGCTCGGCTTCACGTCCGCGCTGATCCC
GAAGGCGAATGCGCCGAAACAGCCGATCGAAGGGCTCAACGTGATGGCCGTCGAGCGGCTCGAGCAGGCGATCGACCGCG
TGCGCGATCTTGAATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A228HY51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.458

100

0.498
  radA Streptococcus mitis SK321

47.162

100

0.472
  radA Streptococcus pneumoniae Rx1

46.855

100

0.472
  radA Streptococcus pneumoniae D39

46.855

100

0.472
  radA Streptococcus pneumoniae R6

46.855

100

0.472
  radA Streptococcus pneumoniae TIGR4

46.855

100

0.472
  radA Streptococcus mitis NCTC 12261

46.827

99.782

0.467

Multiple sequence alignment