Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   GJS30_RS18835 Genome accession   NZ_CP045993
Coordinates   3857641..3858786 (+) Length   381 a.a.
NCBI ID   WP_160216626.1    Uniprot ID   -
Organism   Bacillus velezensis strain LABIM22     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3852641..3863786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJS30_RS18815 (GJS30_18750) - 3853115..3853831 (-) 717 WP_014419126.1 Crp/Fnr family transcriptional regulator -
  GJS30_RS18820 (GJS30_18755) narK 3853929..3855119 (-) 1191 WP_007407632.1 nitrate transporter NarK -
  GJS30_RS18825 (GJS30_18760) argS 3855254..3856924 (-) 1671 WP_007407633.1 arginine--tRNA ligase -
  GJS30_RS18830 (GJS30_18765) - 3856921..3857349 (-) 429 WP_014419128.1 DUF1934 domain-containing protein -
  GJS30_RS18835 (GJS30_18770) rapF 3857641..3858786 (+) 1146 WP_160216626.1 tetratricopeptide repeat protein Regulator
  GJS30_RS18840 (GJS30_18775) - 3858770..3858889 (+) 120 WP_021494790.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  GJS30_RS18845 (GJS30_18780) - 3859087..3859290 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  GJS30_RS18850 (GJS30_18785) - 3859304..3859507 (+) 204 WP_007407641.1 hypothetical protein -
  GJS30_RS18855 (GJS30_18790) - 3859542..3859727 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  GJS30_RS18860 (GJS30_18795) - 3859914..3860195 (+) 282 WP_007407643.1 hypothetical protein -
  GJS30_RS18865 (GJS30_18800) - 3860239..3860751 (+) 513 WP_031304384.1 stage II sporulation protein M -
  GJS30_RS18870 (GJS30_18810) - 3861342..3861560 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  GJS30_RS18875 (GJS30_18815) speB 3861713..3862585 (-) 873 WP_007614448.1 agmatinase -
  GJS30_RS18880 (GJS30_18820) speE 3862644..3863474 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45581.65 Da        Isoelectric Point: 4.5769

>NTDB_id=400094 GJS30_RS18835 WP_160216626.1 3857641..3858786(+) (rapF) [Bacillus velezensis strain LABIM22]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNTAIARQYHDKGISYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKLMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=400094 GJS30_RS18835 WP_160216626.1 3857641..3858786(+) (rapF) [Bacillus velezensis strain LABIM22]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGTCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATACGGCCATAGCCAGACAATATCATGACAAAGGGATATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATTGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.842

99.738

0.567

Multiple sequence alignment