Detailed information NTG0400070

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: GJS30_RS13485
• Genome accession: NZ_CP045993
• Coordinates: 2782728..2785079 (-)
• Length: 783 a.a.
• NCBI ID: WP_179156088.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain LABIM22
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2777728..2790079

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GJS30_RS13450 (GJS30_13400)	-	2778094..2778432 (-)	339	WP_007408270.1	YqxA family protein	-
GJS30_RS13455 (GJS30_13405)	spoIIP	2778449..2779642 (-)	1194	WP_007612673.1	stage II sporulation protein P	-
GJS30_RS13460 (GJS30_13410)	gpr	2779710..2780816 (-)	1107	WP_014418417.1	GPR endopeptidase	-
GJS30_RS13465 (GJS30_13415)	rpsT	2781019..2781285 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GJS30_RS13470 (GJS30_13420)	holA	2781302..2782343 (-)	1042	Protein_2629	DNA polymerase III subunit delta	-
GJS30_RS13475 (GJS30_13425)	-	2782383..2782534 (-)	152	Protein_2630	hypothetical protein	-
GJS30_RS13480 (GJS30_13430)	-	2782575..2782709 (+)	135	WP_003152870.1	YqzM family protein	-
GJS30_RS13485 (GJS30_13435)	comEC	2782728..2785079 (-)	2352	WP_179156088.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GJS30_RS13490 (GJS30_13440)	-	2785080..2785649 (-)	570	WP_003152868.1	ComE operon protein 2	-
GJS30_RS13495 (GJS30_13445)	comEA	2785716..2786330 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
GJS30_RS13500 (GJS30_13450)	comER	2786389..2787210 (+)	822	WP_179156089.1	late competence protein ComER	-
GJS30_RS13505 (GJS30_13455)	-	2787279..2788016 (-)	738	WP_167408922.1	class I SAM-dependent methyltransferase	-
GJS30_RS13510 (GJS30_13460)	rsfS	2788013..2788369 (-)	357	WP_007408260.1	ribosome silencing factor	-
GJS30_RS13515 (GJS30_13465)	yqeK	2788387..2788947 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GJS30_RS13520 (GJS30_13470)	-	2788937..2789506 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
GJS30_RS13525 (GJS30_13475)	yhbY	2789517..2789807 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86665.60 Da
• Isoelectric Point: 9.0098

>NTDB_id=400070 GJS30_RS13485 WP_179156088.1 2782728..2785079(-) (comEC) [Bacillus velezensis strain LABIM22]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKQHAPVIVCLVSFCLYFFLYTVCDAANVTRYQAGSYTE
QVVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEVDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSGQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSIFTLFAALEKGGFLKLRKTA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLSGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=400070 GJS30_RS13485 WP_179156088.1 2782728..2785079(-) (comEC) [Bacillus velezensis strain LABIM22]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCAGCATGCTCCGGTTATTGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGTCGTCATCACTAACATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAGCAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGTCGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTACTGTT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCCGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATTGGTTTCCTTCTTCTTTTACTTTCAGGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTACATGATTTCATTACGTATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCATTTTTACTCTATTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAAACGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGACACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATTAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCATTGGTGCTTTGGACGGTTTTAAGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCGGCACTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

• Competence protein (Competence) (208-469)

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.995	98.595	0.562