Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   HT132_RS12025 Genome accession   NZ_CP054479
Coordinates   2455871..2457745 (-) Length   624 a.a.
NCBI ID   WP_103526898.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain R8-25     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2450871..2462745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT132_RS11995 (HT132_11995) - 2451289..2451966 (-) 678 WP_174532554.1 MBL fold metallo-hydrolase -
  HT132_RS12000 (HT132_12000) - 2452660..2452869 (+) 210 WP_032863736.1 hypothetical protein -
  HT132_RS12005 (HT132_12005) - 2453152..2453670 (+) 519 WP_014417812.1 hypothetical protein -
  HT132_RS12010 (HT132_12010) - 2453676..2453960 (+) 285 WP_014417811.1 hypothetical protein -
  HT132_RS12015 (HT132_12015) - 2454159..2454878 (-) 720 WP_014417810.1 hypothetical protein -
  HT132_RS12020 (HT132_12020) - 2455085..2455626 (-) 542 Protein_2342 tyrosine-type recombinase/integrase -
  HT132_RS12025 (HT132_12025) hexB 2455871..2457745 (-) 1875 WP_103526898.1 DNA mismatch repair endonuclease MutL Machinery gene
  HT132_RS12030 (HT132_12030) hexA 2457761..2460346 (-) 2586 WP_174532752.1 DNA mismatch repair protein MutS Machinery gene
  HT132_RS12035 (HT132_12035) cotE 2460465..2461010 (-) 546 WP_003154111.1 outer spore coat protein CotE -
  HT132_RS12040 (HT132_12040) ymcA 2461263..2461694 (-) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator

Sequence

Protein


Download         Length: 624 a.a.        Molecular weight: 70170.39 Da        Isoelectric Point: 5.9381

>NTDB_id=399889 HT132_RS12025 WP_103526898.1 2455871..2457745(-) (hexB) [Bacillus amyloliquefaciens strain R8-25]
MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDANSTVIEIDIEEAGLSSIRVLDNGEGMETDDCKRAFRRHATSK
IKDENDLFRVRTLGFRGEALPSIASVSHLEIKTSIGEGAGTHLILQGGNIISEQKSSSRKGTEIIVTNLFFNTPARLKYM
KTVHTELGNITDVVNRIALAHPEVSIRLRHQGKNLLQTNGNGDVRHVLAAIYGTAVAKKMIPLHVSSLDFEVKGYIALPE
ITRASRNYMSSVINGRYIKNFPLVKAVHEGYHTLLPIGRHPITFIEITMDPILVDVNVHPSKLEVRLSKETELHELIRDG
IKEVFQKQRLIPSAQLPKKSAPAPAKNEQQFMTFEESAPERKLPEKTPEPSYSPMKLSSVVKEPEPVIIEEEILPNEADH
EVPPELGMPETAAPVSDTPEPEAVSEEIREERVPVMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVGQVE
PEVQDMIVPLTFHYAANEALIIDQHQHELESVGVFLEAFGTNSYIVRCHPAWFPKGEEAELIEEIIQQVLDAKQIDIKKL
REEAAIMMSCKGSIKANRHLRNDEIKALLDDLRRTTDPFTCPHGRPIIIHHSTYEMEKMFKRVM

Nucleotide


Download         Length: 1875 bp        

>NTDB_id=399889 HT132_RS12025 WP_103526898.1 2455871..2457745(-) (hexB) [Bacillus amyloliquefaciens strain R8-25]
TTGGCAAAAGTCATCCAGCTCTCAGATGAACTTTCAAATAAAATAGCGGCCGGCGAGGTGGTCGAACGGCCTGCCTCAGT
CGTTAAAGAATTGGTGGAAAACGCCATCGACGCAAACAGCACAGTCATTGAAATCGACATAGAGGAAGCGGGGCTTTCGT
CGATTCGGGTGCTCGATAACGGCGAAGGGATGGAAACCGATGATTGCAAGCGGGCTTTCCGCCGCCACGCGACCAGTAAA
ATCAAAGATGAAAACGACCTGTTCCGGGTGAGAACACTCGGTTTCCGCGGAGAAGCTTTGCCAAGTATCGCGTCTGTTTC
TCATCTGGAAATCAAAACGAGCATCGGCGAGGGAGCGGGAACCCATCTCATTCTTCAGGGCGGGAATATCATTTCTGAAC
AGAAATCCTCCAGCAGAAAAGGAACGGAGATTATCGTTACCAACCTGTTTTTCAATACACCCGCCAGATTGAAATATATG
AAAACCGTGCATACGGAGCTCGGCAATATTACGGATGTTGTTAACCGGATCGCTCTTGCGCATCCGGAAGTATCGATCCG
TCTCCGTCATCAGGGGAAAAATCTGCTGCAGACAAACGGTAACGGGGATGTGCGCCATGTGCTGGCCGCAATTTACGGGA
CGGCGGTTGCGAAAAAAATGATACCGCTCCATGTCAGCTCGCTTGATTTTGAAGTCAAGGGCTACATTGCGCTTCCGGAA
ATCACTCGTGCATCCAGAAACTACATGTCATCTGTCATTAACGGAAGATACATTAAGAATTTCCCGCTTGTAAAAGCGGT
GCATGAGGGCTATCACACACTTCTGCCGATCGGCCGCCATCCGATTACGTTTATCGAAATTACGATGGACCCGATTTTGG
TCGATGTCAATGTCCATCCCTCGAAGCTTGAAGTCCGCCTCAGCAAAGAGACGGAGCTGCATGAGCTGATCCGTGACGGG
ATAAAGGAAGTGTTTCAAAAGCAGCGGCTTATTCCGAGCGCCCAGCTCCCGAAAAAATCAGCGCCGGCACCGGCCAAAAA
CGAACAGCAATTTATGACATTCGAGGAAAGCGCGCCTGAGCGGAAACTTCCTGAAAAAACGCCGGAGCCGTCTTACTCAC
CAATGAAGCTGAGTTCGGTCGTAAAAGAGCCTGAGCCGGTAATAATAGAAGAAGAGATTCTCCCGAATGAAGCGGATCAC
GAAGTGCCGCCGGAGCTCGGCATGCCGGAAACGGCAGCGCCTGTTTCCGATACTCCGGAACCGGAAGCTGTCTCTGAAGA
AATACGTGAAGAAAGAGTTCCGGTGATGTATCCGATCGGCCAAATGCACGGGACATATATTTTAGCCCAAAACGAAAACG
GCTTGTATATCATTGACCAGCATGCGGCACAAGAACGGATTAAATACGAATATTTCCGGGAGAAAGTCGGGCAGGTCGAG
CCTGAAGTACAGGACATGATCGTGCCGCTCACCTTTCATTACGCGGCAAATGAAGCGCTGATCATTGACCAGCATCAGCA
TGAGCTTGAAAGCGTCGGAGTGTTTTTAGAAGCATTCGGCACGAACAGCTACATCGTCCGCTGCCATCCGGCCTGGTTTC
CAAAAGGGGAAGAAGCTGAACTGATTGAAGAAATCATTCAGCAGGTGCTTGACGCCAAACAGATCGATATTAAAAAACTG
CGGGAGGAAGCGGCCATTATGATGAGCTGCAAAGGCTCAATCAAAGCGAACCGCCATTTGCGAAACGATGAAATCAAAGC
GCTCTTGGACGACCTGCGCCGAACGACAGACCCGTTCACATGCCCGCACGGCCGGCCGATCATTATCCACCACAGCACGT
ATGAGATGGAGAAGATGTTTAAGCGTGTGATGTAG

Domains

Predicted by InterProScan.

MutL C terminal dimerisation domain (MutL_C)

(439-580)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-111)

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

46.357

100

0.479