Detailed information NTG0399802

Bioinformatically predicted

Overview

• Name: disA
• Type: Machinery gene
• Locus tag: HT132_RS00460
• Genome accession: NZ_CP054479
• Coordinates: 63188..64270 (-)
• Length: 360 a.a.
• NCBI ID: WP_003156401.1
• Uniprot ID: A0AAI8HS26
• Organism: Bacillus amyloliquefaciens strain R8-25
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 58188..69270

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HT132_RS00435 (HT132_00435)	cysE	58287..58940 (-)	654	WP_014416745.1	serine O-acetyltransferase	-
HT132_RS00440 (HT132_00440)	gltX	59251..60702 (-)	1452	WP_017419470.1	glutamate--tRNA ligase	-
HT132_RS00445 (HT132_00445)	ispF	60794..61270 (-)	477	WP_003156407.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
HT132_RS00450 (HT132_00450)	ispD	61263..61961 (-)	699	WP_053285121.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
HT132_RS00455 (HT132_00455)	-	61974..63074 (-)	1101	WP_003156403.1	PIN/TRAM domain-containing protein	-
HT132_RS00460 (HT132_00460)	disA	63188..64270 (-)	1083	WP_003156401.1	DNA integrity scanning diadenylate cyclase DisA	Machinery gene
HT132_RS00465 (HT132_00465)	radA/sms	64274..65653 (-)	1380	WP_014416742.1	DNA repair protein RadA	Machinery gene
HT132_RS00470 (HT132_00470)	clpC	65747..68179 (-)	2433	WP_007410388.1	ATP-dependent protease ATP-binding subunit ClpC	Regulator
HT132_RS00475 (HT132_00475)	-	68176..69267 (-)	1092	WP_003156398.1	protein arginine kinase	-

Sequence

Protein

• Length: 360 a.a.
• Molecular weight: 40671.21 Da
• Isoelectric Point: 5.5321

>NTDB_id=399802 HT132_RS00460 WP_003156401.1 63188..64270(-) (disA) [Bacillus amyloliquefaciens strain R8-25]
MEKEKKGAKQELDLSAILQFVAPGTPLRAGIENVLRANTGGLIVVGYNDKVKQVVDGGFHINTSFAPAHLYELAKMDGAI
ILSDSGQKILYANTQLMPEATISSSETGMRHRTAERVAKQTGCLVIAISERRNVITLYQDSMKYTLKDIGFILTKANQAI
QTLEKYKTILDKTITALNALEFEELVTFSDVLSVMHRYEMVLRIKNEINMYIKELGTEGHLIRLQVIELITDMEEEAALF
IKDYVKEKIKDPFVLLKELQDMSSYDLLDDSILFKLLGYPASTNLDDYVLPRGYRLLNKIPRLPMPIVENVVEAFGFLPS
ITEATVEELDDVEGIGEVRAQKIKKGLKRLQEKHYIDRQL

Nucleotide

• Length: 1083 bp

>NTDB_id=399802 HT132_RS00460 WP_003156401.1 63188..64270(-) (disA) [Bacillus amyloliquefaciens strain R8-25]
ATGGAAAAAGAGAAAAAAGGGGCGAAACAAGAGCTTGACCTGTCTGCAATTCTGCAGTTCGTTGCACCGGGCACCCCGCT
CAGAGCCGGGATAGAGAATGTACTTCGGGCAAATACAGGCGGCCTGATTGTTGTCGGATACAATGATAAGGTAAAGCAAG
TCGTCGATGGCGGTTTTCATATTAATACATCATTTGCGCCCGCTCACTTATACGAGCTGGCCAAAATGGACGGTGCCATT
ATTTTAAGTGATTCCGGCCAGAAAATTCTTTATGCGAATACGCAGCTTATGCCGGAAGCGACCATTTCTTCTTCAGAAAC
GGGAATGAGGCACCGTACGGCGGAACGGGTGGCGAAGCAGACCGGCTGTCTCGTCATTGCCATTTCTGAGCGCAGAAATG
TCATTACGTTATATCAGGACAGCATGAAGTACACACTAAAGGACATAGGATTTATTTTAACAAAGGCAAATCAGGCCATT
CAAACGCTTGAGAAGTATAAAACCATTTTAGACAAAACGATTACGGCTTTAAACGCATTAGAGTTTGAGGAGCTTGTGAC
ATTCAGTGACGTGCTGTCCGTCATGCATCGGTATGAAATGGTGCTCCGAATAAAAAATGAAATCAATATGTATATTAAAG
AACTCGGAACGGAAGGCCACTTAATCCGTCTGCAGGTGATTGAACTGATTACGGACATGGAAGAAGAGGCGGCACTTTTC
ATCAAAGACTATGTCAAAGAAAAAATCAAAGATCCGTTCGTGCTGTTAAAAGAACTTCAGGATATGTCCAGCTATGATCT
GCTTGATGATTCCATTCTGTTTAAGCTGCTTGGATATCCGGCCTCTACGAATCTTGATGATTATGTGCTCCCGAGAGGAT
ACAGGCTGCTGAACAAAATCCCGCGTCTGCCGATGCCGATTGTTGAAAATGTGGTTGAGGCGTTCGGATTTTTGCCAAGC
ATAACGGAAGCCACAGTTGAAGAGCTTGATGATGTCGAAGGTATCGGCGAAGTGCGCGCCCAAAAAATCAAAAAAGGCTT
AAAACGCCTTCAGGAAAAACACTATATAGATAGACAGCTTTAA

Domains

Predicted by InterProScan.

• DisA bacterial checkpoint controller nucleotide-binding (DAC) (29-141)

• DisA bacterial checkpoint controller linker region (DisA-linker) (146-284)

3D structure

• AlphaFold DB: A0AAI8HS26

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
disA	Bacillus subtilis subsp. subtilis str. 168	94.444	100	0.944