Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilH   Type   Machinery gene
Locus tag   HTY60_RS09220 Genome accession   NZ_CP054415
Coordinates   1729732..1730094 (+) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain 205     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1724732..1735094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HTY60_RS09190 (HTY60_09190) flgD 1725647..1726078 (+) 432 WP_013352262.1 flagellar hook assembly protein FlgD -
  HTY60_RS09195 (HTY60_09195) flgG 1726103..1726879 (+) 777 WP_013352263.1 flagellar basal body rod protein FlgG -
  HTY60_RS09200 (HTY60_09200) - 1726919..1727134 (+) 216 WP_013352264.1 flagellar FlbD family protein -
  HTY60_RS09205 (HTY60_09205) fliL 1727131..1727550 (+) 420 WP_013352265.1 flagellar basal body-associated protein FliL -
  HTY60_RS09210 (HTY60_09210) fliM 1727584..1728582 (+) 999 WP_013352266.1 flagellar motor switch protein FliM -
  HTY60_RS09215 (HTY60_09215) fliY 1728572..1729705 (+) 1134 WP_013352267.1 flagellar motor switch phosphatase FliY -
  HTY60_RS09220 (HTY60_09220) pilH 1729732..1730094 (+) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  HTY60_RS09225 (HTY60_09225) fliZ 1730109..1730762 (+) 654 WP_013352268.1 flagella biosynthesis regulatory protein FliZ -
  HTY60_RS09230 (HTY60_09230) fliP 1730755..1731420 (+) 666 WP_013352269.1 flagellar type III secretion system pore protein FliP -
  HTY60_RS09235 (HTY60_09235) fliQ 1731435..1731704 (+) 270 WP_013352270.1 flagellar biosynthesis protein FliQ -
  HTY60_RS09240 (HTY60_09240) fliR 1731711..1732490 (+) 780 WP_013352271.1 flagellar biosynthetic protein FliR -
  HTY60_RS09245 (HTY60_09245) flhB 1732490..1733569 (+) 1080 WP_013352272.1 flagellar biosynthesis protein FlhB -

Sequence


Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=399484 HTY60_RS09220 WP_003154238.1 1729732..1730094(+) (pilH) [Bacillus amyloliquefaciens strain 205]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=399484 HTY60_RS09220 WP_003154238.1 1729732..1730094(+) (pilH) [Bacillus amyloliquefaciens strain 205]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAGGATATTTTAGTGAAGAACGGTTT
TGACGTTGTAGCTGAAGCTTCGGACGGAGCACAGGCAGTCGAAAAATTCAAAGAGCATTCACCCGACCTTGTGACAATGG
ATATTACAATGCCTGAAATGGACGGGATTACCGCATTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATCATG
TGTTCGGCTATGGGGCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAGCCATTCCA
AGCAGACCGCGTGCTTGAAGCCATTAATAAAACATTAAGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383