Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SGLY_RS01580 Genome accession   NC_015172
Coordinates   327036..328382 (+) Length   448 a.a.
NCBI ID   WP_013623535.1    Uniprot ID   F0SWX4
Organism   Syntrophobotulus glycolicus DSM 8271     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 322036..333382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGLY_RS01560 (Sgly_0293) - 322363..322836 (+) 474 WP_013623531.1 CtsR family transcriptional regulator -
  SGLY_RS01565 (Sgly_0294) - 322863..323390 (+) 528 WP_013623532.1 UvrB/UvrC motif-containing protein -
  SGLY_RS01570 (Sgly_0295) - 323380..324432 (+) 1053 WP_013623533.1 protein arginine kinase -
  SGLY_RS01575 (Sgly_0296) - 324482..327007 (+) 2526 WP_013623534.1 ATP-dependent Clp protease ATP-binding subunit -
  SGLY_RS01580 (Sgly_0297) radA 327036..328382 (+) 1347 WP_013623535.1 DNA repair protein RadA Machinery gene
  SGLY_RS01585 (Sgly_0298) - 328545..328937 (-) 393 WP_013623536.1 hypothetical protein -
  SGLY_RS01590 (Sgly_0299) - 329148..330320 (+) 1173 WP_041444925.1 PIN/TRAM domain-containing protein -
  SGLY_RS01595 (Sgly_0300) ispD 330307..331023 (+) 717 WP_013623538.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  SGLY_RS01600 (Sgly_0301) ispF 331014..331490 (+) 477 WP_013623539.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  SGLY_RS01605 (Sgly_0302) gltX 331570..333030 (+) 1461 WP_013623540.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 48826.42 Da        Isoelectric Point: 5.9659

>NTDB_id=39948 SGLY_RS01580 WP_013623535.1 327036..328382(+) (radA) [Syntrophobotulus glycolicus DSM 8271]
MARIKTKYFCKECGQESPRWLGKCPACGEWNTFAEEKISVSSSSLTDRGAVKAAPLSEITSMEIERLDTGNGELNRVLGG
GAVPGSFVLVSGEPGIGKSTLLLQLADYLSKENNVLYVSGEESARQIKIRADRLGISSSKLHILTETILHRIREIVLQDN
YQVLVIDSIQTMMLEDLESAPGSVSQVREGAAFLMKLAKENHIIIFLVGHITKDGNIAGPRVLEHMVDTVLCFEGDQHYI
YRLLRTIKNRFGPANEIGVFEMRGSGLCEVANTSEVFLGSRTSDVAGSAVAVTMEGSRPLLVEIQALVTPTGFNPPRRTV
NGMDYHRLLMLLAVLDKRIGNVFYARDVFINIAGGLRIDDPAVDLAIIGAILSAMNDTPLPKLALIGEVGLTGELRGVSQ
IEQRIKEAEKLGFEGCMIPSVNNQAALKSDCLIYAAGTLEEAKKYLKF

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=39948 SGLY_RS01580 WP_013623535.1 327036..328382(+) (radA) [Syntrophobotulus glycolicus DSM 8271]
GTGGCAAGGATTAAGACGAAGTATTTCTGTAAAGAATGCGGGCAGGAAAGTCCCCGCTGGCTTGGTAAATGCCCTGCATG
TGGAGAGTGGAATACCTTTGCCGAGGAGAAAATTTCTGTTTCCTCCTCAAGTTTGACTGACAGGGGAGCTGTTAAAGCAG
CCCCCCTGTCAGAGATTACATCCATGGAAATAGAGAGACTGGATACGGGGAACGGGGAATTGAACAGAGTGCTGGGAGGA
GGGGCCGTGCCCGGCAGTTTTGTACTTGTCAGCGGAGAGCCTGGTATCGGCAAATCCACCCTGTTGCTTCAGCTTGCGGA
TTATTTGTCTAAAGAGAATAATGTTTTGTATGTTTCCGGTGAAGAATCGGCGCGCCAGATTAAAATCAGGGCGGACCGTT
TGGGGATTTCCTCATCAAAGCTACATATTCTTACGGAGACAATACTTCACAGAATAAGAGAAATCGTTCTTCAGGACAAT
TATCAGGTATTGGTGATTGATTCGATTCAAACGATGATGCTGGAGGACCTGGAATCTGCACCGGGAAGCGTCAGTCAGGT
TCGGGAAGGCGCCGCTTTTCTGATGAAGCTGGCCAAGGAGAACCATATCATCATTTTCCTGGTTGGTCATATTACCAAGG
ATGGAAATATCGCAGGACCGCGTGTTCTTGAACACATGGTGGATACTGTACTTTGTTTTGAAGGAGATCAGCATTATATC
TATCGTCTTTTGCGGACAATCAAAAACCGTTTTGGCCCGGCAAACGAAATCGGTGTTTTTGAAATGAGAGGAAGCGGGCT
TTGTGAAGTTGCCAATACTTCGGAGGTTTTTCTCGGCAGCAGGACGTCAGATGTTGCCGGTTCGGCGGTAGCCGTAACAA
TGGAAGGGTCGCGTCCTTTGCTGGTTGAAATCCAAGCCCTTGTGACACCGACAGGATTCAATCCTCCCAGAAGGACGGTC
AATGGCATGGATTATCACAGGCTGCTCATGCTTCTGGCTGTGCTGGACAAGAGAATCGGCAATGTCTTTTATGCCCGTGA
TGTCTTTATTAATATTGCAGGGGGACTGAGGATAGATGATCCCGCTGTGGATCTTGCAATTATTGGAGCCATTCTTTCCG
CGATGAATGATACTCCGCTCCCCAAATTGGCGCTCATTGGAGAAGTGGGCTTAACAGGAGAGCTGCGGGGAGTATCGCAG
ATAGAGCAGCGTATTAAAGAGGCCGAGAAGCTGGGGTTTGAGGGCTGCATGATTCCAAGTGTCAATAATCAGGCGGCATT
AAAAAGCGATTGCCTGATTTACGCTGCGGGAACATTGGAAGAGGCAAAAAAATACTTGAAGTTTTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(57-215)

Subunit ChlI of Mg-chelatase (ChlI)

(339-422)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F0SWX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

54.186

95.982

0.52
  radA Streptococcus mitis NCTC 12261

51.887

94.643

0.491
  radA Streptococcus pneumoniae Rx1

51.887

94.643

0.491
  radA Streptococcus pneumoniae D39

51.887

94.643

0.491
  radA Streptococcus pneumoniae R6

51.887

94.643

0.491
  radA Streptococcus pneumoniae TIGR4

51.887

94.643

0.491
  radA Streptococcus mitis SK321

51.887

94.643

0.491

Multiple sequence alignment