Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GJE18_RS01170 Genome accession   NZ_CP045927
Coordinates   236464..237834 (+) Length   456 a.a.
NCBI ID   WP_165886067.1    Uniprot ID   -
Organism   Staphylococcus agnetis strain 1379     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 231464..242834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJE18_RS01150 (GJE18_01145) - 231799..232260 (+) 462 WP_037566295.1 CtsR family transcriptional regulator -
  GJE18_RS01155 (GJE18_01150) - 232272..232790 (+) 519 WP_060552317.1 UvrB/UvrC motif-containing protein -
  GJE18_RS01160 (GJE18_01155) - 232783..233787 (+) 1005 WP_085622202.1 protein arginine kinase -
  GJE18_RS01165 (GJE18_01160) - 233800..236262 (+) 2463 WP_107368324.1 ATP-dependent Clp protease ATP-binding subunit -
  GJE18_RS01170 (GJE18_01165) radA 236464..237834 (+) 1371 WP_165886067.1 DNA repair protein RadA Machinery gene
  GJE18_RS01175 (GJE18_01170) - 237849..238910 (+) 1062 WP_107368323.1 PIN/TRAM domain-containing protein -
  GJE18_RS01180 (GJE18_01175) ispD 238913..239608 (+) 696 WP_060552321.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GJE18_RS01185 (GJE18_01180) ispF 239601..240077 (+) 477 WP_060552322.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GJE18_RS01190 (GJE18_01185) gltX 240166..241620 (+) 1455 WP_107368322.1 glutamate--tRNA ligase -
  GJE18_RS01195 (GJE18_01190) cysE 241950..242576 (+) 627 WP_168429109.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50112.69 Da        Isoelectric Point: 8.0159

>NTDB_id=399296 GJE18_RS01170 WP_165886067.1 236464..237834(+) (radA) [Staphylococcus agnetis strain 1379]
MAKKKITFECTACGYQSPKWMGKCPNCGAWNSMEESLEQKTAHPKHGVRAQKTHAKVQTLNEIKNEQTPRILTKSEEFNR
VLGGGIVQGSLVLIGGDPGIGKSTLLLQMCATLSKTKKVLYITGEESLNQTKLRADRLDEDASALNVFAETDLEVIHDAV
KKVEPELIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
AHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVPNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIIIATASSFKDQAVDGLDCFIGEVGLTGEV
RRVSRIEQRVQEAAKLGFKRVIIPKTNIGGWTFPENIEVIGVSSTNEALKHALKQR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=399296 GJE18_RS01170 WP_165886067.1 236464..237834(+) (radA) [Staphylococcus agnetis strain 1379]
GTGGCCAAAAAAAAGATTACATTTGAGTGTACTGCATGCGGATACCAGTCTCCAAAATGGATGGGGAAATGCCCAAATTG
CGGTGCATGGAATTCAATGGAAGAATCATTGGAACAAAAAACAGCTCACCCTAAACATGGCGTGCGTGCGCAAAAAACAC
ATGCAAAAGTGCAAACATTAAATGAAATTAAAAATGAACAGACACCACGTATTCTTACTAAAAGTGAAGAATTTAACCGT
GTGCTTGGAGGCGGAATTGTTCAAGGTTCTCTCGTGCTCATCGGGGGCGATCCAGGTATCGGTAAATCAACACTCCTCCT
TCAAATGTGTGCTACGTTATCTAAAACTAAGAAAGTACTTTATATTACCGGTGAAGAATCGCTAAATCAAACGAAACTAC
GCGCCGACCGTTTAGATGAAGATGCTAGTGCACTCAATGTGTTTGCAGAAACGGATCTTGAAGTCATTCATGATGCAGTA
AAAAAAGTGGAGCCTGAGTTAATCGTTGTAGATTCCATTCAAACCATTTATCACCCAGAAATCAGTTCTGCACCGGGGTC
AGTATCTCAAGTGAGGGAGAGTACACAAAGCCTCATGCATATTGCCAAACAAATGAATATCGCTACATTTATCGTTGGAC
ACGTTACGAAAGAAGGCCAAATTGCGGGTCCTCGATTGTTAGAACATATGGTGGATACTGTGCTCTATTTTGAAGGCGAT
GCACATCATGCATATCGCATTTTGCGTGCGGTGAAGAACCGGTTTGGTTCTACAAATGAAATGGGAATATTTGAAATGAA
ACAATCAGGTTTAAAAAGCGTGCCGAATCCGTCCGAAATGTTTTTAGAAGAACGCTCTACAAATGTACCAGGTTCTACGA
TTGTGGCAACAATGGAAGGAACCCGTCCGTTACTGATTGAAGTGCAAGCTTTAGTCACACCAACAACTTTTAACAATCCT
AGACGAATGGCTACAGGTATAGATCATAATCGCCTAAGTTTGTTAATGGCTGTTTTAGAGAAAAAAGAAGGGTATTTACT
CCAGCAACAAGATGCATATATTAAAGTAGCAGGTGGTGTCAAATTAAGTGAACCCGCTGTTGATTTAGGTATCATTATTG
CGACAGCTTCGAGTTTTAAAGATCAAGCTGTTGATGGATTAGATTGTTTTATAGGTGAAGTAGGTTTAACCGGGGAAGTG
CGCAGAGTCTCCCGTATTGAACAAAGGGTTCAAGAAGCGGCGAAGTTGGGATTCAAACGCGTCATTATTCCGAAAACCAA
TATCGGTGGGTGGACATTCCCAGAAAATATAGAAGTAATCGGTGTGTCTTCAACAAATGAAGCATTAAAGCATGCCTTAA
AACAAAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(344-427)

AAA domain (AAA_25)

(62-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.304

100

0.669
  radA Streptococcus pneumoniae Rx1

59.471

99.561

0.592
  radA Streptococcus pneumoniae D39

59.471

99.561

0.592
  radA Streptococcus pneumoniae R6

59.471

99.561

0.592
  radA Streptococcus pneumoniae TIGR4

59.471

99.561

0.592
  radA Streptococcus mitis NCTC 12261

59.471

99.561

0.592
  radA Streptococcus mitis SK321

59.471

99.561

0.592

Multiple sequence alignment