Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GI584_RS00785 Genome accession   NZ_CP045915
Coordinates   157162..158532 (+) Length   456 a.a.
NCBI ID   WP_100362649.1    Uniprot ID   A0A5Q2TFI7
Organism   Gracilibacillus salitolerans strain SCU50     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 152162..163532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GI584_RS00765 (GI584_00765) - 152231..152695 (+) 465 WP_100362653.1 CtsR family transcriptional regulator -
  GI584_RS00770 (GI584_00770) - 152708..153250 (+) 543 WP_100362652.1 UvrB/UvrC motif-containing protein -
  GI584_RS00775 (GI584_00775) - 153247..154323 (+) 1077 WP_153789925.1 protein arginine kinase -
  GI584_RS00780 (GI584_00780) clpC 154325..156751 (+) 2427 WP_153789926.1 ATP-dependent protease ATP-binding subunit ClpC -
  GI584_RS00785 (GI584_00785) radA 157162..158532 (+) 1371 WP_100362649.1 DNA repair protein RadA Machinery gene
  GI584_RS00790 (GI584_00790) - 158694..159797 (+) 1104 WP_100362648.1 PIN/TRAM domain-containing protein -
  GI584_RS00795 (GI584_00795) ispD 160532..161227 (+) 696 WP_100362665.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GI584_RS00800 (GI584_00800) ispF 161224..161700 (+) 477 WP_153789927.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GI584_RS00805 (GI584_00805) gltX 161787..163253 (+) 1467 WP_100362646.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49899.34 Da        Isoelectric Point: 6.8383

>NTDB_id=399002 GI584_RS00785 WP_100362649.1 157162..158532(+) (radA) [Gracilibacillus salitolerans strain SCU50]
MAKRKSKFVCQSCGYESPKWMGKCPGCNEWNTLVEEVTAAKTTRGNWATGATTAQKPEKITSIQLQEEPRIKTKMQELNR
VLGGGIVAGSLVLIGGDPGIGKSTLLLQVSSQLSQRDLDVLYISGEESARQTKLRADRLDISSDRLYVLSETNLVDISNQ
IDQLKPGFVVIDSIQTIYKEEVTSAPGSVSQVRECTSELMRIAKQNHIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYFEG
ERHHTFRILRGVKNRFGSTNEMGIFEMKEEGLTEVLNPSEIFLEERSQGVAGSTIVASMEGTRPVLVEIQALISPTSFGN
PRRMATGIDHNRVPLLMAVLEKRVGLMLQNQDAYVKVAGGVKLDEPAIDLAVAISIASSFRDRAPHTDDVLVGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRAIIPAKNMDGWTVPASIEIIGINSVQEAIKVALGD

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=399002 GI584_RS00785 WP_100362649.1 157162..158532(+) (radA) [Gracilibacillus salitolerans strain SCU50]
TTGGCTAAGCGAAAATCTAAATTTGTTTGTCAATCTTGTGGTTATGAATCACCCAAATGGATGGGGAAATGCCCAGGGTG
TAACGAGTGGAATACTTTAGTAGAAGAGGTCACAGCTGCAAAAACAACAAGAGGTAATTGGGCAACAGGTGCTACCACAG
CGCAAAAACCGGAAAAAATAACAAGTATCCAGTTACAGGAAGAACCAAGAATTAAAACCAAAATGCAAGAGTTAAATCGT
GTGCTTGGTGGTGGTATTGTAGCAGGTTCACTAGTGTTAATCGGTGGAGATCCAGGTATTGGGAAATCCACGTTATTACT
GCAAGTTTCTAGTCAATTATCACAACGCGACTTAGATGTGCTATACATTTCTGGAGAAGAATCAGCAAGGCAAACAAAGC
TGAGAGCAGATAGATTGGATATTTCATCAGACAGACTATATGTACTTTCGGAAACGAATCTAGTAGATATCTCAAACCAA
ATTGATCAATTGAAACCAGGTTTTGTTGTTATTGATTCGATTCAAACCATTTATAAAGAAGAAGTGACTAGTGCTCCAGG
TAGTGTTTCACAAGTTCGCGAATGTACAAGTGAACTGATGCGCATTGCGAAGCAAAATCATATACCTATTTTTATAGTTG
GACATGTGACGAAAGAAGGCTCTATTGCCGGTCCTAGACTGCTTGAGCATATGGTAGATGCCGTATTGTATTTTGAAGGA
GAAAGACACCATACCTTCCGAATTTTACGGGGAGTAAAAAACCGGTTTGGCAGTACGAACGAAATGGGTATTTTTGAAAT
GAAAGAAGAGGGGTTAACAGAAGTATTAAACCCATCCGAAATTTTTTTAGAAGAACGATCACAAGGTGTGGCTGGTTCAA
CTATCGTCGCATCGATGGAAGGAACAAGACCAGTACTAGTGGAAATCCAGGCATTAATATCTCCTACCAGCTTTGGTAAT
CCACGGCGAATGGCGACCGGAATTGATCATAATCGAGTTCCATTATTAATGGCTGTATTAGAAAAAAGGGTAGGCTTAAT
GTTGCAAAACCAAGATGCATATGTAAAGGTAGCGGGTGGCGTGAAATTAGATGAGCCTGCCATTGACTTAGCGGTTGCGA
TTAGTATTGCTTCAAGCTTTAGAGATCGAGCTCCACATACAGATGATGTGTTAGTCGGTGAAGTTGGACTTACGGGAGAA
GTACGAAGAGTATCGCGAATTGAACAGCGTGTACAAGAAGCCGCTAAATTAGGGTTTAAGAGAGCCATCATCCCCGCTAA
AAATATGGATGGTTGGACGGTCCCTGCGTCAATTGAAATTATTGGCATTAATTCCGTACAAGAGGCAATTAAAGTAGCAT
TAGGAGATTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(69-220)

Subunit ChlI of Mg-chelatase (ChlI)

(352-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q2TFI7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.586

100

0.768
  radA Streptococcus pneumoniae Rx1

62.335

99.561

0.621
  radA Streptococcus pneumoniae D39

62.335

99.561

0.621
  radA Streptococcus pneumoniae R6

62.335

99.561

0.621
  radA Streptococcus pneumoniae TIGR4

62.335

99.561

0.621
  radA Streptococcus mitis NCTC 12261

62.335

99.561

0.621
  radA Streptococcus mitis SK321

62.335

99.561

0.621

Multiple sequence alignment