Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPE52_RS17230 Genome accession   NZ_CP054345
Coordinates   3574229..3574669 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain SCU-176     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3569229..3579669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE52_RS17210 (HPE52_17210) aroP 3570229..3571599 (+) 1371 WP_001540704.1 aromatic amino acid transporter AroP -
  HPE52_RS17215 (HPE52_17215) ampE 3571642..3572496 (-) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  HPE52_RS17220 (HPE52_17220) ampD 3572493..3573044 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPE52_RS17225 (HPE52_17225) nadC 3573132..3574025 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPE52_RS17230 (HPE52_17230) pilA 3574229..3574669 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  HPE52_RS17235 (HPE52_17235) pilB 3574679..3576064 (+) 1386 WP_174165881.1 type II secretion system protein GspE Machinery gene
  HPE52_RS17240 (HPE52_17240) hofC 3576054..3577256 (+) 1203 WP_001540700.1 protein transport protein HofC -
  HPE52_RS17245 (HPE52_17245) guaC 3577291..3578334 (-) 1044 WP_001217338.1 GMP reductase -
  HPE52_RS24670 - 3578490..3578534 (-) 45 WP_120795372.1 protein YacM -
  HPE52_RS17250 (HPE52_17250) coaE 3578559..3579179 (+) 621 WP_001269524.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=398888 HPE52_RS17230 WP_000360914.1 3574229..3574669(+) (pilA) [Escherichia coli strain SCU-176]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=398888 HPE52_RS17230 WP_000360914.1 3574229..3574669(+) (pilA) [Escherichia coli strain SCU-176]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363