Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   APZ19_RS01700 Genome accession   NZ_CP045859
Coordinates   348309..349325 (+) Length   338 a.a.
NCBI ID   WP_054824717.1    Uniprot ID   A0AAP9K8V9
Organism   Vibrio owensii strain SH14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343309..354325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APZ19_RS01685 (APZ19_01685) - 343681..344409 (-) 729 WP_054824719.1 redoxin family protein -
  APZ19_RS01690 (APZ19_01690) oxyR 344585..345493 (+) 909 WP_033005806.1 DNA-binding transcriptional regulator OxyR -
  APZ19_RS01695 (APZ19_01695) - 345560..348130 (-) 2571 WP_054824718.1 penicillin-binding protein 1A -
  APZ19_RS01700 (APZ19_01700) pilM 348309..349325 (+) 1017 WP_054824717.1 type IV pilus assembly protein PilM Machinery gene
  APZ19_RS01705 (APZ19_01705) pilN 349309..349887 (+) 579 WP_054824716.1 PilN domain-containing protein Machinery gene
  APZ19_RS01710 (APZ19_01710) pilO 349880..350473 (+) 594 WP_009705519.1 type 4a pilus biogenesis protein PilO Machinery gene
  APZ19_RS01715 (APZ19_01715) pilP 350463..350978 (+) 516 WP_005438430.1 pilus assembly protein PilP Machinery gene
  APZ19_RS01720 (APZ19_01720) pilQ 351013..352761 (+) 1749 WP_045412013.1 type IV pilus secretin PilQ Machinery gene
  APZ19_RS01725 (APZ19_01725) aroK 352951..353469 (+) 519 WP_005438434.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37987.30 Da        Isoelectric Point: 6.1873

>NTDB_id=398736 APZ19_RS01700 WP_054824717.1 348309..349325(+) (pilM) [Vibrio owensii strain SH14]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKERIVAENNTINHQEIVKTLKEMRKELPIRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVMQAFAHQSPFPVEELSLDFVKVDTNEQMGGTESYQVFATRREVVESRVDTLTKSGLKTV
LVDVHSQSLGHIWKLTAERFPEKNKHCLLDIGAMSSSFTMFTEQGELFHKEFPCGTRTTNDLSQHDPLSDDIQTATEEFN
RQMVERVKRQMQLYTSFNGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLSLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDDAASH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=398736 APZ19_RS01700 WP_054824717.1 348309..349325(+) (pilM) [Vibrio owensii strain SH14]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCTTTATTGGGTTACAAAGAAATAATACTTAAGGAACGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAGAAAAGAGCTCCCAATTCGAGCTAGAAAGGTCGCAATTTCGGTTCCAGACAATGCT
GTGATCAGCAAAAAGCTACAAATAGAGCAGAACCTAGAAGAAAGCGAAAAGGAATTTGCGGTGATGCAAGCCTTTGCGCA
TCAATCTCCCTTTCCGGTTGAAGAGCTCAGCCTCGATTTTGTAAAGGTTGATACGAATGAGCAGATGGGCGGCACAGAGA
GCTACCAAGTGTTTGCAACACGCAGAGAGGTGGTGGAAAGCCGAGTGGATACATTAACGAAGTCTGGCCTAAAAACCGTG
CTGGTGGATGTGCACTCACAAAGTCTTGGTCATATATGGAAGCTAACCGCAGAACGTTTTCCTGAAAAGAATAAGCATTG
CTTGTTAGACATTGGTGCGATGAGTAGCTCTTTCACTATGTTTACCGAGCAAGGTGAGCTATTCCATAAGGAATTTCCTT
GCGGTACTCGCACAACAAACGATTTGTCTCAACACGATCCACTCAGTGACGATATCCAAACTGCAACTGAAGAATTCAAT
CGACAAATGGTTGAACGTGTTAAGCGTCAGATGCAGCTCTATACCTCGTTCAATGGTAGCCAAGATATCAAAGGTATTTG
GCTTTCCGGTGAGGGGGCGGCTACGCCAATGTTAGCTGAGGAACTATCGAGTCAATTGGCACTAGAGTGTGAACTTCTCA
ATCCTCTTAGTTTGTTTGAAATGAAAGTCTCTAAACGTAAACGCCGAGCGGCAGATTGGCAGCATTTCACCGCAGCTGCA
GGTTTAGCGGTACGCGGTATTCAGTGGTTGGGAGGTGACGATGCTGCATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

88.462

100

0.885
  pilM Vibrio cholerae strain A1552

47.839

100

0.491

Multiple sequence alignment