Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GII23_RS21490 Genome accession   NZ_CP045858
Coordinates   4570353..4572443 (-) Length   696 a.a.
NCBI ID   WP_061339735.1    Uniprot ID   A0A9X7VB66
Organism   Stutzerimonas balearica strain EC28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4565353..4577443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII23_RS21475 (GII23_21410) - 4567074..4568672 (-) 1599 WP_165564211.1 AAA family ATPase -
  GII23_RS21480 (GII23_21415) aroB 4568675..4569778 (-) 1104 WP_165564080.1 3-dehydroquinate synthase -
  GII23_RS21485 (GII23_21420) aroK 4569803..4570348 (-) 546 WP_061339736.1 shikimate kinase AroK -
  GII23_RS21490 (GII23_21425) pilQ 4570353..4572443 (-) 2091 WP_061339735.1 type IV pilus secretin PilQ Machinery gene
  GII23_RS21495 (GII23_21430) - 4572499..4573026 (-) 528 WP_165564081.1 pilus assembly protein PilP -
  GII23_RS21500 (GII23_21435) pilO 4573023..4573646 (-) 624 WP_043218303.1 type 4a pilus biogenesis protein PilO -
  GII23_RS21505 (GII23_21440) - 4573643..4574215 (-) 573 WP_043218300.1 PilN domain-containing protein -
  GII23_RS21510 (GII23_21445) pilM 4574215..4575279 (-) 1065 WP_061337879.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 74827.80 Da        Isoelectric Point: 4.7533

>NTDB_id=398729 GII23_RS21490 WP_061339735.1 4570353..4572443(-) (pilQ) [Stutzerimonas balearica strain EC28]
MNSTLSRVGVAMLALLASPALLAANLNKLDVASLPGDRVELKLAFDEPVSKPRGYTLEQPARIALDLPGVSNKLGVKNRE
LGLGNARSVTVVEAQGRTRLIVNLASLVPYATRVEGNELFIVLGENEGAVAPIASAAASPVASRPAPVAAKMVGKAISNI
DFRRGEDGAGNVVITLSDASVGSTIEEQGGKINVKFDKAQLPEALRVRLDVQDFATPVRFVDATSSSGGASVAIEPSGRY
DYLAYQTDNQLTISVKPLSEDEAEKRKSERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVP
WDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRRELIQVNYAKAADIARLFQSVTENGSGA
TEERGSIAVDDRTNSIIAYQTQERLDELRRIVMQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLGTGRKWSAWGKNG
NLTVEDAEGGGQELKWPANTPFVDMGAVGSTSGIGLGFVTDNAILDLQLSAMEKTGNGEVVSQPKVVTSDKETAKILKGS
EIPYQEASSSGATSTSFKEAALSLEVTPQITPDDRIIMEVKVTKDAPDFGNAVDGVPPISKNEVNAKVLVNDGETIVIGG
VFSNTQSKSVDKVPFLGDLPYLGRLFRRDTVTNEKSELLIFLTPRIMSNQAIAVSH

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=398729 GII23_RS21490 WP_061339735.1 4570353..4572443(-) (pilQ) [Stutzerimonas balearica strain EC28]
ATGAATAGCACCCTCTCTCGTGTGGGCGTCGCCATGCTGGCGCTGCTTGCCTCGCCTGCGCTGTTGGCAGCGAATTTGAA
CAAGCTTGATGTGGCATCGCTGCCGGGAGATCGGGTCGAGCTAAAGCTGGCCTTCGATGAGCCGGTCAGCAAGCCGCGCG
GCTATACGCTAGAGCAGCCGGCCCGCATTGCGCTGGACTTGCCAGGCGTTTCGAACAAGCTTGGTGTGAAGAACCGGGAG
CTGGGCCTTGGCAACGCGCGTAGCGTAACGGTGGTCGAAGCGCAGGGGCGTACCCGTCTGATCGTAAATCTTGCGTCGCT
CGTGCCCTATGCCACTCGCGTCGAAGGTAACGAGCTGTTCATTGTTCTCGGCGAGAACGAGGGGGCGGTTGCCCCAATTG
CGTCCGCGGCTGCGTCCCCGGTAGCGAGCCGGCCTGCGCCGGTCGCCGCCAAGATGGTCGGCAAAGCCATCAGTAATATC
GATTTCCGACGAGGCGAAGACGGAGCAGGCAATGTCGTGATCACCCTGTCCGACGCATCGGTCGGCTCGACCATCGAGGA
GCAGGGCGGAAAGATCAATGTGAAGTTTGACAAGGCACAGCTGCCGGAAGCGCTACGTGTCCGCCTGGATGTGCAGGACT
TTGCCACGCCGGTGCGCTTCGTGGATGCCACCAGCTCATCCGGCGGCGCCAGTGTGGCGATCGAACCGAGCGGCCGATAT
GACTATCTCGCATACCAGACGGACAACCAGCTGACCATTAGCGTGAAGCCGCTGAGTGAAGACGAGGCCGAGAAGCGCAA
GTCTGAGCGCTTTGCATACAGCGGGGAAAAGCTGTCGCTGAACTTCCAAGATATCGATGTGCGCTCGGTGCTGCAGCTGA
TTGCCGACTTCACCGATCTGAACCTGGTGGCCAGTGACACCGTGCAGGGCAACATTACCCTGCGCCTGCAGAATGTGCCG
TGGGATCAGGCGCTTGACCTAGTGCTCAAGACGAAGGGATTGGATAAGCGCCAGGTAGGTAATGTGCTGCTTGTGGCGCC
TGCGGACGAGATCGCAGCCCGTGAGCGGCAGGAGCTCGAGTCGCAGCGGCAAATCGCCGAGCTGGCGCCGCTGCGGCGCG
AGCTGATTCAGGTCAACTATGCCAAGGCTGCCGACATTGCGCGTCTGTTCCAGTCGGTCACCGAGAATGGCAGCGGGGCA
ACCGAGGAGCGCGGCTCCATCGCAGTCGATGACCGCACCAACAGCATCATCGCCTACCAGACCCAGGAGCGGCTGGACGA
ACTGCGCCGCATAGTCATGCAGCTGGATATCCCGGTTCGCCAGGTGATGATCGAGGCACGGATCGTCGAGGCGAACGTCG
ATTACGACAAGAGTCTTGGGGTGCGCTGGGGCGGAAACCTCGGTACTGGCCGCAAATGGAGCGCCTGGGGTAAGAATGGA
AATCTGACGGTGGAAGACGCGGAGGGTGGTGGCCAGGAACTCAAGTGGCCCGCCAACACGCCATTCGTAGACATGGGGGC
GGTCGGCTCGACGTCCGGCATTGGTCTTGGCTTCGTTACGGACAATGCGATTCTAGACCTTCAGCTTTCTGCCATGGAAA
AGACCGGAAATGGTGAGGTCGTTTCGCAGCCGAAGGTCGTTACCTCGGACAAGGAAACAGCCAAGATCCTGAAGGGCTCC
GAAATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCGACCAGCACCTCGTTTAAAGAGGCCGCATTGTCCTTGGAAGTTAC
GCCTCAGATCACGCCTGACGATCGGATCATCATGGAGGTGAAAGTCACCAAGGACGCGCCTGATTTTGGCAATGCCGTAG
ACGGGGTGCCCCCGATCAGCAAGAACGAGGTCAATGCCAAAGTGCTGGTCAACGACGGCGAGACGATTGTGATCGGTGGC
GTCTTCTCGAATACGCAGAGCAAGTCGGTCGACAAGGTACCGTTCCTGGGGGACTTGCCTTACCTCGGTCGCCTGTTCCG
TCGTGACACTGTAACCAACGAAAAATCGGAACTGCTGATCTTCCTGACTCCGCGTATCATGAGTAACCAAGCGATAGCTG
TGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-441)

Bacterial type II and III secretion system protein (Secretin)

(531-687)

Secretin and TonB N terminus short domain (STN)

(301-347)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.095

100

0.764
  comQ Acinetobacter baylyi ADP1

41.453

100

0.418
  pilQ Acinetobacter baumannii strain A118

39.373

100

0.415
  pilQ Acinetobacter baumannii D1279779

39.373

100

0.415
  pilQ Legionella pneumophila strain ERS1305867

38.596

98.276

0.379
  pilQ Legionella pneumophila strain Lp02

38.304

98.276

0.376

Multiple sequence alignment