Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS093_RS10050 Genome accession   NZ_CP054282
Coordinates   2113607..2114047 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain IDR1900005638-01-02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2108607..2119047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS093_RS10030 (HS093_10020) aroP 2109605..2110978 (+) 1374 WP_001331235.1 aromatic amino acid transporter AroP -
  HS093_RS10035 (HS093_10025) ampE 2111021..2111875 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HS093_RS10040 (HS093_10030) ampD 2111872..2112423 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HS093_RS10045 (HS093_10035) nadC 2112511..2113404 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HS093_RS10050 (HS093_10040) pilA 2113607..2114047 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  HS093_RS10055 (HS093_10045) pilB 2114057..2115442 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  HS093_RS10060 (HS093_10050) hofC 2115432..2116634 (+) 1203 WP_000157234.1 protein transport protein HofC -
  HS093_RS10065 (HS093_10055) guaC 2116669..2117712 (-) 1044 WP_001217338.1 GMP reductase -
  HS093_RS10070 - 2117868..2117912 (-) 45 WP_120795372.1 protein YacM -
  HS093_RS10075 (HS093_10060) coaE 2117937..2118557 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=398659 HS093_RS10050 WP_000360895.1 2113607..2114047(+) (pilA) [Escherichia coli strain IDR1900005638-01-02]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=398659 HS093_RS10050 WP_000360895.1 2113607..2114047(+) (pilA) [Escherichia coli strain IDR1900005638-01-02]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363