Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACAV_RS02330 Genome accession   NC_015138
Coordinates   521304..522893 (+) Length   529 a.a.
NCBI ID   WP_013592973.1    Uniprot ID   -
Organism   Paracidovorax avenae ATCC 19860     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 516304..527893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAV_RS02305 (Acav_0475) argS 516530..518344 (+) 1815 WP_013592968.1 arginine--tRNA ligase -
  ACAV_RS02310 (Acav_0476) - 518359..519009 (+) 651 WP_013592969.1 SPOR domain-containing protein -
  ACAV_RS02315 (Acav_0477) - 519083..519739 (+) 657 WP_013592970.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACAV_RS02320 (Acav_0478) lptA 519860..520510 (+) 651 WP_013592971.1 lipopolysaccharide transport periplasmic protein LptA -
  ACAV_RS02325 (Acav_0479) lptB 520513..521304 (+) 792 WP_013592972.1 LPS export ABC transporter ATP-binding protein -
  ACAV_RS02330 (Acav_0480) rpoN/rpoN1 521304..522893 (+) 1590 WP_013592973.1 RNA polymerase factor sigma-54 Machinery gene
  ACAV_RS02335 (Acav_0481) - 522921..523466 (-) 546 WP_081463152.1 TspO/MBR family protein -
  ACAV_RS02340 (Acav_0482) - 523535..525382 (-) 1848 WP_013592975.1 methyl-accepting chemotaxis protein -
  ACAV_RS02345 (Acav_0483) - 525502..527364 (-) 1863 WP_013592976.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58057.25 Da        Isoelectric Point: 4.6032

>NTDB_id=39863 ACAV_RS02330 WP_013592973.1 521304..522893(+) (rpoN/rpoN1) [Paracidovorax avenae ATCC 19860]
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFGLPQADAPVSDADRGAEESLY
TPASGTAEVAASSPGEGASEGTETPEAPDAPDWEGDGTVEMAPDDGEWGGEAPARTRNQMGDEDDTDATELAREHESLTD
YLHRQALSLRLSEVDRAALRFLIESLNDDGYLDESLESLAVSLAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
ECMALQLRALQEDGDAEPEVLQAALRICQQPLEMLARRDVRRLAQGCGASEECTRAAMALIARLEPRPGRAFAQVERNII
VPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGALRGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAI
VDRQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=39863 ACAV_RS02330 WP_013592973.1 521304..522893(+) (rpoN/rpoN1) [Paracidovorax avenae ATCC 19860]
ATGAAGCCAGGACTGTCGCTGCGCATCTCGCAGCATCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTGGAGCTGTCGCAGGAAGTCGAGCAGATGCTCGACGAGAACCCCTTCCTGGAGCGCAACCCGGACGAGG
CTCCGCGCGAGGAGTTCGGCCTGCCGCAGGCGGATGCGCCGGTGAGCGATGCCGACCGCGGTGCGGAAGAGTCCTTGTAC
ACGCCGGCCAGCGGTACGGCGGAGGTTGCGGCCTCGTCGCCGGGCGAGGGCGCATCCGAAGGCACCGAGACACCGGAAGC
CCCCGATGCGCCGGACTGGGAGGGCGACGGCACCGTCGAGATGGCGCCCGACGACGGCGAGTGGGGCGGCGAGGCGCCCG
CCCGCACCCGCAACCAGATGGGGGACGAAGACGATACGGACGCGACCGAACTCGCGCGCGAGCACGAGTCGCTCACCGAT
TACCTGCACCGGCAGGCGCTGTCTCTGCGCCTGTCGGAGGTGGACCGCGCGGCGCTGCGCTTCCTGATCGAATCGCTGAA
CGACGACGGTTACCTGGACGAATCGCTGGAGTCGCTGGCCGTGTCGCTGGCAGGCGAGGAAGACCCCGAACAGGTCGAGG
AACTGGTGCACCGCTTCACGGTGGCGCTGCGGCTGTTGCAGAGCCTGGAGCCCGTGGGCGTGGGCGCGCGCGGCCTGAGC
GAATGCATGGCGCTGCAGTTGCGGGCCCTGCAGGAAGACGGCGACGCGGAGCCCGAAGTGCTGCAGGCGGCACTGCGCAT
CTGCCAGCAGCCGCTGGAGATGCTGGCACGGCGCGACGTGCGCCGGCTCGCCCAGGGCTGCGGTGCGAGCGAGGAATGCA
CGCGTGCGGCCATGGCGCTCATCGCGCGGCTGGAGCCCCGGCCAGGCCGGGCCTTTGCGCAGGTCGAGCGCAACATCATC
GTTCCCGACGTGATCGTGAAGAAGAGCAGTGGCCGAGGGGCCGCGCATGCGTTCACCGTGCAGCTCAATCCGGACGTCAT
GCCGCGGCTGCGCGTGCATGACATCTATGCGGGGGCACTGCGCGGCAACCGCGGTGGCGAGGGGCACCAGGCGCTGCAGC
AGCGGCTGCAGGAGGCGCGATGGTTCATCAAGAACATCCAGCAGCGGTTCGACACCATCCTGCGCGTGTCGCGCGCCATC
GTGGACCGGCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGGCCGCTCGTGCTGCGCGACATCGCCGACGAGCT
GGGACTGCACGAATCGACGATCAGCCGGGTGACCACGGCCAAGTACATGGCGACTCCCCAGGGCACCTACGAGCTGAAGT
ATTTCTTCGGCTCGGGGCTGGGCACGGAGACGGGCGGCAATGCGTCGAGCACGGCCGTGCGCGCGCTGATCAAGCAGTTC
GTCGCGTCGGAGAGCCCGGCCAAGCCGCTGTCGGACAGCCAGATCGCGGAGATGCTCAAGGAGCAGGGCATCGAGTGCGC
GCGCCGCACGGTCGCCAAGTACCGCGAGGCGCTGAAGATCGCGCCGGCCAACCTGCGCAAGGCGCTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.412

100

0.516

Multiple sequence alignment