Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   HR070_RS10325 Genome accession   NZ_CP054236
Coordinates   2187364..2188791 (-) Length   475 a.a.
NCBI ID   WP_000980604.1    Uniprot ID   -
Organism   Escherichia coli strain EcPF5     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2182364..2193791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR070_RS10290 (HR070_10280) hisL 2182948..2182998 (-) 51 WP_001364200.1 his operon leader peptide -
  HR070_RS10295 (HR070_10285) yefM 2183281..2183532 (+) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  HR070_RS10300 (HR070_10290) yoeB 2183529..2183783 (+) 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  HR070_RS10305 (HR070_10295) yeeZ 2183866..2184690 (+) 825 WP_000754733.1 SDR family oxidoreductase -
  HR070_RS10310 (HR070_10300) yeeY 2184736..2185665 (+) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  HR070_RS10315 (HR070_10305) yoeI 2185880..2185942 (+) 63 WP_010723108.1 membrane protein YoeI -
  HR070_RS10320 (HR070_10310) plaP 2185932..2187290 (+) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  HR070_RS10325 (HR070_10315) sbcB 2187364..2188791 (-) 1428 WP_000980604.1 exodeoxyribonuclease I Machinery gene
  HR070_RS10330 (HR070_10320) dacD 2189000..2190166 (+) 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  HR070_RS10335 (HR070_10325) sbmC 2190285..2190758 (+) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  HR070_RS10340 (HR070_10330) yeeA 2190956..2192014 (+) 1059 WP_001200890.1 FUSC family protein -
  HR070_RS10345 (HR070_10335) tmaR 2192186..2192515 (+) 330 WP_000450409.1 DUF496 family protein -
  HR070_RS25880 (HR070_10340) - 2192616..2192798 (-) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  HR070_RS25560 - 2192918..2192998 (+) 81 WP_023441679.1 hypothetical protein -
  HR070_RS10360 (HR070_10350) - 2193287..2193367 (-) 81 Protein_2044 hypothetical protein -
  HR070_RS10365 (HR070_10355) yeeW 2193413..2193607 (-) 195 WP_000988601.1 DUF5983 family protein -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54442.73 Da        Isoelectric Point: 5.4720

>NTDB_id=398582 HR070_RS10325 WP_000980604.1 2187364..2188791(-) (sbcB) [Escherichia coli strain EcPF5]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=398582 HR070_RS10325 WP_000980604.1 2187364..2188791(-) (sbcB) [Escherichia coli strain EcPF5]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAAC
GAAGCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGAGAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583