Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HR070_RS06275 Genome accession   NZ_CP054236
Coordinates   1300665..1301105 (-) Length   146 a.a.
NCBI ID   WP_000360915.1    Uniprot ID   A0A0H2V436
Organism   Escherichia coli strain EcPF5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1295665..1306105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR070_RS06250 (HR070_06245) coaE 1296155..1296775 (-) 621 WP_001269525.1 dephospho-CoA kinase -
  HR070_RS06255 - 1296800..1296844 (+) 45 WP_120795372.1 protein YacM -
  HR070_RS06260 (HR070_06250) guaC 1297000..1298043 (+) 1044 WP_001217338.1 GMP reductase -
  HR070_RS06265 (HR070_06255) hofC 1298078..1299280 (-) 1203 WP_000157242.1 protein transport protein HofC -
  HR070_RS06270 (HR070_06260) pilB 1299270..1300655 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  HR070_RS06275 (HR070_06265) pilA 1300665..1301105 (-) 441 WP_000360915.1 prepilin peptidase-dependent pilin Machinery gene
  HR070_RS06280 (HR070_06270) nadC 1301309..1302202 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HR070_RS06285 (HR070_06275) ampD 1302290..1302841 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HR070_RS06290 (HR070_06280) ampE 1302838..1303692 (+) 855 WP_000172013.1 beta-lactamase regulator AmpE -
  HR070_RS06295 (HR070_06285) aroP 1303735..1305105 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.77 Da        Isoelectric Point: 4.5237

>NTDB_id=398569 HR070_RS06275 WP_000360915.1 1300665..1301105(-) (pilA) [Escherichia coli strain EcPF5]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCTIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=398569 HR070_RS06275 WP_000360915.1 1300665..1301105(-) (pilA) [Escherichia coli strain EcPF5]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCACTATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V436

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363